ORMDL3 (NM_139280) Human Tagged ORF Clone
SKU
RC202279
ORMDL3 (Myc-DDK-tagged)-Human ORM1-like 3 (S. cerevisiae) (ORMDL3)
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TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Target Symbol | ORMDL3 |
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Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC202279 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGTGGGCACAGCGCACAGCGAGGTGAACCCCAACACGCGGGTGATGAACAGCCGTGGCATCTGGC TCTCCTACGTGCTGGCCATCGGTCTCCTCCACATCGTGCTGCTGAGCATCCCGTTTGTGAGTGTCCCTGT CGTCTGGACCCTCACCAACCTCATTCACAACATGGGCATGTATATCTTCCTGCACACGGTGAAGGGGACA CCCTTTGAGACCCCGGACCAGGGCAAGGCGAGGCTGCTAACCCACTGGGAGCAGATGGATTATGGGGTCC AGTTCACGGCCTCTCGGAAGTTCTTGACCATCACACCCATCGTGCTGTACTTCCTCACCAGCTTCTACAC TAAGTACGACCAGATCCATTTTGTGCTCAACACCGTGTCCCTGATGAGCGTGCTTATCCCCAAGCTGCCC CAGCTCCACGGAGTCCGGATTTTTGGAATCAATAAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202279 protein sequence
Red=Cloning site Green=Tags(s) MNVGTAHSEVNPNTRVMNSRGIWLSYVLAIGLLHIVLLSIPFVSVPVVWTLTNLIHNMGMYIFLHTVKGT PFETPDQGKARLLTHWEQMDYGVQFTASRKFLTITPIVLYFLTSFYTKYDQIHFVLNTVSLMSVLIPKLP QLHGVRIFGINKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_139280 |
ORF Size | 459 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_139280.4 |
RefSeq Size | 2169 bp |
RefSeq ORF | 462 bp |
Locus ID | 94103 |
UniProt ID | Q8N138 |
Cytogenetics | 17q21.1 |
Protein Families | Transmembrane |
MW | 17.5 kDa |
Summary | Negative regulator of sphingolipid synthesis. May indirectly regulate endoplasmic reticulum-mediated Ca(+2) signaling.[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC202279L1 | Lenti ORF clone of Human ORM1-like 3 (S. cerevisiae) (ORMDL3), Myc-DDK-tagged | 10 ug |
$450.00
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RC202279L2 | Lenti ORF clone of Human ORM1-like 3 (S. cerevisiae) (ORMDL3), mGFP tagged | 10 ug |
$450.00
|
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RC202279L3 | Lenti ORF clone of Human ORM1-like 3 (S. cerevisiae) (ORMDL3), Myc-DDK-tagged | 10 ug |
$450.00
|
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RC202279L4 | Lenti ORF clone of Human ORM1-like 3 (S. cerevisiae) (ORMDL3), mGFP tagged | 10 ug |
$450.00
|
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RG202279 | ORMDL3 (tGFP-tagged) - Human ORM1-like 3 (S. cerevisiae) (ORMDL3) | 10 ug |
$489.00
|
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SC321992 | ORMDL3 (untagged)-Human ORM1-like 3 (S. cerevisiae) (ORMDL3) | 10 ug |
$150.00
|
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.