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SPINT2 (NM_021102) Human Tagged ORF Clone

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SKU
RC202044
SPINT2 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPINT2
Synonyms DIAR3; HAI-2; HAI2; Kop; PB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202044 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGCTGTGCGGGCTGAGGCGGAGCCGGGCGTTTCTCGCCCTGCTGGGATCGCTGCTCCTCTCTG
GGGTCCTGGCGGCCGACCGAGAACGCAGCATCCACGACTTCTGCCTGGTGTCGAAGGTGGTGGGCAGATG
CCGGGCCTCCATGCCTAGGTGGTGGTACAATGTCACTGACGGATCCTGCCAGCTGTTTGTGTATGGGGGC
TGTGACGGAAACAGCAATAATTACCTGACCAAGGAGGAGTGCCTCAAGAAATGTGCCACTGTCACAGAGA
ATGCCACGGGTGACCTGGCCACCAGCAGGAATGCAGCGGATTCCTCTGTCCCAAGTGCTCCCAGAAGGCA
GGATTCTGAAGACCACTCCAGCGATATGTTCAACTATGAAGAATACTGCACCGCCAACGCAGTCACTGGG
CCTTGCCGTGCATCCTTCCCACGCTGGTACTTTGACGTGGAGAGGAACTCCTGCAATAACTTCATCTATG
GAGGCTGCCGGGGCAATAAGAACAGCTACCGCTCTGAGGAGGCCTGCATGCTCCGCTGCTTCCGCCAGCA
GGAGAATCCTCCCCTGCCCCTTGGCTCAAAGGTGGTGCTTCTGGCGGGGCTGTTCGTGATGGTGTTGATC
CTCTTCCTGGGAGCCTCCATGGTCTACCTGATCCGGGTGGCACGGAGGAACCAGGAGCGTGCCCTGCGCA
CCGTCTGGAGCTCCGGAGATGACAAGGAGCAGCTGGTGAAGAACACATATGTCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202044 protein sequence
Red=Cloning site Green=Tags(s)

MAQLCGLRRSRAFLALLGSLLLSGVLAADRERSIHDFCLVSKVVGRCRASMPRWWYNVTDGSCQLFVYGG
CDGNSNNYLTKEECLKKCATVTENATGDLATSRNAADSSVPSAPRRQDSEDHSSDMFNYEEYCTANAVTG
PCRASFPRWYFDVERNSCNNFIYGGCRGNKNSYRSEEACMLRCFRQQENPPLPLGSKVVLLAGLFVMVLI
LFLGASMVYLIRVARRNQERALRTVWSSGDDKEQLVKNTYVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021102
ORF Size 756 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021102.4
RefSeq Size 1817 bp
RefSeq ORF 759 bp
Locus ID 10653
UniProt ID O43291, B4DLU1
Cytogenetics 19q13.2
Domains KU
Protein Families Transmembrane
MW 28.2 kDa
Summary This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2009
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Documents
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"NM_021102" in other vectors (7)
SKU Description Size Price
RC202044L1 Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, Myc-DDK-tagged 10 ug
$600.00
RC202044L2 Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, mGFP tagged 10 ug
$600.00
RC202044L3 Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, Myc-DDK-tagged 10 ug
$600.00
RC202044L4 Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, mGFP tagged 10 ug
$600.00
RG202044 SPINT2 (tGFP-tagged) - Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a 10 ug
$489.00 $500.00
SC112961 SPINT2 (untagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a 10 ug
$300.00
SC322322 SPINT2 (untagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a 10 ug
$300.00

Plasmid Map for RC202044

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202044
Circular map for RC202044

file-alt Citations

SPINT2 mutations in the Kunitz domain 2 found in SCSD patients inactivate HAI-2 as prostasin inhibitor via abnormal protein folding and N-glycosylation
Huang, Wang, Bernard et al
Hum Mol Genet (2024)
Cited by 6
AI Summary: OriGene’s pCMV-HAI2 plasmid (CAT#: RC202044) was used as the wild-type HAI-2 expression construct for generating and studying SPINT2 Kunitz domain 2 mutants in this research on SCSD pathogenesis.
Snippet: A wide-type HAI-2 plasmid (pCMV-HAI2) was purchased from OriGene Technologies, Inc (CAT#: RC202044). The R148H, R148C and Y163C mutant HAI-2 plasmids were generated in the pCMV-HAI2 plasmid using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instruction. The sequence of the pri
The Role of HAI-2 in the Intestine Depends on Its Correct Subcellular Targeting
Huang
Thesis (2024)
Snippet: 2.6. Plasmid preparation The wide-type HAI-2 construct (pCMV-HAI2) was derived from the pCMV-entry-HAI-2 + Myc/DDK-tagged plasmid which contains a cDNA coding for the HAI-2 protein with Myc and DDK fusion tags, purchased from OriGene Technologies (CAT#: RC202044) by removing the
Targeted HAI-2 deletion causes excessive proteolysis with prolonged active prostasin and depletion of HAI-1 monomer in intestinal but not epidermal epithelial cells
Barndt, Lee, Huang et al
Hum Mol Genet (2021)
Cited by 5
AI Summary: OriGene’s SPINT2/HAI-2 (NM_021102) Human Myc-DDK-Tagged ORF clone (RC202044) was used in this study to enable exogenous expression of HAI-2 in cell models for investigating the effects of HAI-2 deletion on proteolytic activity.
Snippet: Exogenous protein expression with the Tet-On system Plasmids RC202044, a SPINT2/HAI-2 (NM_021102) Human Myc-DDK-Tagged ORF clone, was purchased from Origene (Rockville, MD). pLVX-Tet3G (TetON) and pLVX-TRE3G were purchased from Clontech
Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis
Wu, Feng, Lu et al
J. Clin. Invest. (2017)
AI Summary: OriGene products, specifically plasmids expressing human matriptase, Flag-tagged human matriptase, and Flag-tagged human HAI-2, were used to investigate the roles of these proteins in the regulation of EpCAM cleavage and intestinal epithelial homeostasis in this study.
Snippet: pcDNA3-HAEpCAM has been described (12). Plasmids expressing human matriptase, Flag-tagged human matriptase, and Flag-tagged human HAI-2 were purchased from OriGene
Showing 1-4 of 4 papers.
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