LYPLA2 (NM_007260) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LYPLA2 |
| Synonyms | APT-2; APT2; DJ886K2.4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007260 |
| ORF Size | 693 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007260.3 |
| RefSeq Size | 1648 bp |
| RefSeq ORF | 696 bp |
| Locus ID | 11313 |
| UniProt ID | O95372, A0A140VJC9 |
| Cytogenetics | 1p36.11 |
| Domains | abhydrolase_2 |
| Protein Pathways | Glycerophospholipid metabolism |
| MW | 24.7 kDa |
| Summary | Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007260" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202021L1 | Lenti ORF clone of Human lysophospholipase II (LYPLA2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202021L2 | Lenti ORF clone of Human lysophospholipase II (LYPLA2), mGFP tagged | 10 ug |
$750.00
|
|
| RC202021L3 | Lenti ORF clone of Human lysophospholipase II (LYPLA2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202021L4 | Lenti ORF clone of Human lysophospholipase II (LYPLA2), mGFP tagged | 10 ug |
$750.00
|
|
| RG202021 | LYPLA2 (tGFP-tagged) - Human lysophospholipase II (LYPLA2) | 10 ug |
$650.00
|
|
| SC115622 | LYPLA2 (untagged)-Human lysophospholipase II (LYPLA2) | 10 ug |
$450.00
|
|
| SC324073 | LYPLA2 (untagged)-Human lysophospholipase II (LYPLA2) | 10 ug |
$450.00
|
|
Citations
S-acylation of NLRP3 provides a nigericin sensitive gating mechanism that controls access to the Golgi
Williams, Peden
Elife (2024) 13 DOI: 10.7554/eLife.94302
Williams, Peden
Elife (2024) 13 DOI: 10.7554/eLife.94302
AI Summary: OriGene products APT1-Myc-FLAG (Cat#: RC202029) and APT2-Myc-FLAG (Cat#: RC202021) plasmids were used in this study to investigate the role of thioesterase enzymes in regulating the S-acylation cycle of NLRP3 at the Golgi.
Snippet: 69 Chicken polyclonal Anti-goat Alexa-594 Thermo Fisher Scientific Cat# A-11058 RRID:AB_2534105 Donkey polyclonal Plasmids PLASMID REFERENCE BACKBONE APT1-Myc-FLAG Origene Cat#: RC202029 pCMV6 APT2-Myc-FLAG Origene Cat#: RC202021 pCMV6 EGFP-+8 pre Yeung et al., 2008 pEGFP HA-ZDHHC1-23 Fukata et al., 2004 pEF-Bos mCherry-P
Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria
Speck, Bhatt, Zhang et al
Cell Rep (2023) 42 (11), 113389 DOI: 10.1016/j.celrep.2023.113389
Speck, Bhatt, Zhang et al
Cell Rep (2023) 42 (11), 113389 DOI: 10.1016/j.celrep.2023.113389
Cited by 19
Lysophospholipases Cooperate to Mediate Lipid Homeostasis and Lysophospholipid Signaling
Wepy, Galligan, Kingsley et al
J. Lipid Res. (2018)
Wepy, Galligan, Kingsley et al
J. Lipid Res. (2018)
AI Summary: OriGene Technologies contributed cDNA clones used for the expression and purification of recombinant LYPLA1 and LYPLA2 enzymes, enabling the authors to characterize these lysophospholipases’ structure and substrate specificity.
Snippet: 16:0-d3 LPC was synthesized as described in Supplemental Information. Recombinant enzyme was expressed and purified as described previously, using cDNA from OriGene Technologies (Rockville, MD) (41). HIS-Select nickel affinity beads were from Sigma
Showing 1-3 of 3 papers.
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