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PGRMC1 (NM_006667) Human Tagged ORF Clone

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SKU
RC201918
PGRMC1 (Myc-DDK-tagged)-Human progesterone receptor membrane component 1 (PGRMC1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Other products for "PGRMC1 or NM_006667"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PGRMC1
Synonyms Dap1; HPR6.6; IZA; MPR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201918 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGAGGATGTGGTGGCGACTGGCGCCGACCCAAGCGATCTGGAGAGCGGCGGGCTGCTGCATG
AGATTTTCACGTCGCCGCTCAACCTGCTGCTGCTTGGCCTCTGCATCTTCCTGCTCTACAAGATCGTGCG
CGGGGACCAGCCGGCGGCCAGCGGCGACAGCGACGACGACGAGCCGCCCCCTCTGCCCCGCCTCAAGCGG
CGCGACTTCACCCCCGCCGAGCTGCGGCGCTTCGACGGCGTCCAGGACCCGCGCATACTCATGGCCATCA
ACGGCAAGGTGTTCGATGTGACCAAAGGCCGCAAATTCTACGGGCCCGAGGGGCCGTATGGGGTCTTTGC
TGGAAGAGATGCATCCAGGGGCCTTGCCACATTTTGCCTGGATAAGGAAGCACTGAAGGATGAGTACGAT
GACCTTTCTGACCTCACTGCTGCCCAGCAGGAGACTCTGAGTGACTGGGAGTCTCAGTTCACTTTCAAGT
ATCATCACGTGGGCAAACTGCTGAAGGAGGGGGAGGAGCCCACTGTGTACTCAGATGAGGAAGAACCAAA
AGATGAGAGTGCCCGGAAAAATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201918 protein sequence
Red=Cloning site Green=Tags(s)

MAAEDVVATGADPSDLESGGLLHEIFTSPLNLLLLGLCIFLLYKIVRGDQPAASGDSDDDEPPPLPRLKR
RDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYD
DLSDLTAAQQETLSDWESQFTFKYHHVGKLLKEGEEPTVYSDEEEPKDESARKND

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006667
ORF Size 585 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006667.5
RefSeq Size 1931 bp
RefSeq ORF 588 bp
Locus ID 10857
UniProt ID O00264
Cytogenetics Xq24
Domains heme_1
Protein Families Druggable Genome, Nuclear Hormone Receptor, Transmembrane
MW 21.7 kDa
Summary This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_006667" in other vectors (6)
SKU Description Size Price
RC201918L1 Lenti ORF clone of Human progesterone receptor membrane component 1 (PGRMC1), Myc-DDK-tagged 10 ug
$600.00
RC201918L2 Lenti ORF clone of Human progesterone receptor membrane component 1 (PGRMC1), mGFP tagged 10 ug
$600.00
RC201918L3 Lenti ORF clone of Human progesterone receptor membrane component 1 (PGRMC1), Myc-DDK-tagged 10 ug
$600.00
RC201918L4 Lenti ORF clone of Human progesterone receptor membrane component 1 (PGRMC1), mGFP tagged 10 ug
$600.00
RG201918 PGRMC1 (tGFP-tagged) - Human progesterone receptor membrane component 1 (PGRMC1) 10 ug
$489.00 $500.00
SC115946 PGRMC1 (untagged)-Human progesterone receptor membrane component 1 (PGRMC1) 10 ug
$300.00

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