CLN6 (NM_017882) Human Tagged ORF Clone

CAT#: RC201904

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CLN6 (Myc-DDK-tagged)-Human ceroid-lipofuscinosis, neuronal 6, late infantile, variant (CLN6)



  "NM_017882" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00


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Size
    • 10 ug


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Other products for "CLN6"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CLN6
Synonyms CLN4A; HsT18960; nclf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201904 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGACGCGGAGGCGGCAGCACCTGGGAGCGACGGGCGGCCCAGGCGCGCAGCTGGGCGCCTCCT
TCCTGCAGGCCAGGCATGGCTCTGTGAGCGCTGATGAGGCTGCCCGCACGGCTCCCTTCCACCTCGACCT
CTGGTTCTACTTCACACTGCAGAACTGGGTTCTGGACTTTGGGCGTCCCATTGCCATGCTGGTATTCCCT
CTCGAGTGGTTTCCACTCAACAAGCCCAGTGTTGGGGACTACTTCCACATGGCCTACAACGTCATCACGC
CCTTTCTCTTGCTCAAGCTCATCGAGCGGTCCCCCCGCACCCTGCCACGCTCCATCACGTACGTGAGCAT
CATCATCTTCATCATGGGTGCCAGCATCCACCTGGTGGGTGACTCTGTCAACCACCGCCTGCTCTTCAGT
GGCTACCAGCACCACCTGTCTGTCCGTGAGAACCCCATCATCAAGAATCTCAAGCCGGAGACGCTGATCG
ACTCCTTTGAGCTGCTCTACTATTATGATGAGTACCTGGGTCACTGCATGTGGTACATCCCCTTCTTCCT
CATCCTCTTCATGTACTTCAGCGGCTGCTTTACTGCCTCTAAAGCTGAGAGCTTGATTCCAGGGCCTGCC
CTGCTCCTGGTGGCACCCAGTGGCCTGTACTACTGGTACCTGGTCACCGAGGGCCAGATCTTCATCCTCT
TCATCTTCACCTTCTTCGCCATGCTGGCCCTCGTCCTGCACCAGAAGCGCAAGCGCCTCTTCCTGGACAG
CAACGGCCTCTTCCTCTTCTCCTCCTTCGCACTGACCCTCTTGCTTGTGGCGCTCTGGGTCGCCTGGCTG
TGGAATGACCCTGTTCTCAGGAAGAAGTACCCGGGTGTCATCTACGTCCCTGAGCCCTGGGCTTTCTACA
CCCTTCACGTCAGCAGTCGGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201904 protein sequence
Red=Cloning site Green=Tags(s)

MEATRRRQHLGATGGPGAQLGASFLQARHGSVSADEAARTAPFHLDLWFYFTLQNWVLDFGRPIAMLVFP
LEWFPLNKPSVGDYFHMAYNVITPFLLLKLIERSPRTLPRSITYVSIIIFIMGASIHLVGDSVNHRLLFS
GYQHHLSVRENPIIKNLKPETLIDSFELLYYYDEYLGHCMWYIPFFLILFMYFSGCFTASKAESLIPGPA
LLLVAPSGLYYWYLVTEGQIFILFIFTFFAMLALVLHQKRKRLFLDSNGLFLFSSFALTLLLVALWVAWL
WNDPVLRKKYPGVIYVPEPWAFYTLHVSSRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017882
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017882.3
RefSeq Size 2258 bp
RefSeq ORF 936 bp
Locus ID 54982
UniProt ID Q9NWW5
Cytogenetics 15q23
Protein Families Transmembrane
MW 35.9 kDa
Gene Summary This gene is one of eight which have been associated with neuronal ceroid lipofuscinoses (NCL). Also referred to as Batten disease, NCL comprises a class of autosomal recessive, neurodegenerative disorders affecting children. The genes responsible likely encode proteins involved in the degradation of post-translationally modified proteins in lysosomes. The primary defect in NCL disorders is thought to be associated with lysosomal storage function. [provided by RefSeq, Oct 2008]

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