Tapasin Related Protein (TAPBPL) (NM_018009) Human Tagged ORF Clone

SKU RC201899

TAPBPL (Myc-DDK-tagged)-Human TAP binding protein-like (TAPBPL)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Tapasin Related Protein
• Synonyms: TAPBP-R; TAPBPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201899 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCACACAGGAGGGCTGGTGCCTGCTGCTCTGCCTGGCTCTATCTGGAGCAGCAGAAACCAAGCCCC; ACCCAGCAGAGGGGCAGTGGCGGGCAGTGGACGTGGTCCTAGACTGTTTCCTGGTGAAGGACGGTGCGCA; CCGTGGAGCTCTCGCCAGCAGTGAGGACAGGGCAAGGGCCTCCCTTGTGCTGAAGCAGGTGCCAGTGCTG; GACGATGGCTCCCTGGAGGACTTCACCGATTTCCAAGGGGGCACACTGGCCCAAGATGACCCACCTATTA; TCTTTGAGGCCTCAGTGGACCTGGTCCAGATTCCCCAGGCCGAGGCCTTGCTCCATGCTGACTGCAGTGG; GAAGGAGGTGACCTGTGAGATCTCCCGCTACTTTCTCCAGATGACAGAGACCACTGTTAAGACAGCAGCT; TGGTTCATGGCCAACGTGCAGGTCTCTGGAGGGGGACCTAGCATCTCCTTGGTGATGAAGACTCCCAGGG; TCGCCAAGAATGAGGTGCTCTGGCACCCAACGCTGAACTTGCCACTGAGCCCCCAGGGGACTGTGCGAAC; TGCAGTGGAGTTCCAGGTGATGACACAGACCCAATCCCTGAGCTTCCTGCTGGGGTCCTCAGCCTCCTTG; GACTGTGGCTTCTCCATGGCACCGGGCTTGGACCTCATCAGTGTGGAGTGGCGACTGCAGCACAAGGGCA; GGGGTCAGTTGGTGTACAGCTGGACCGCAGGGCAGGGGCAGGCTGTGCGGAAGGGCGCTACCCTGGAGCC; TGCACAACTGGGCATGGCCAGGGATGCCTCCCTCACCCTGCCCGGCCTCACTATACAGGACGAGGGGACC; TACATTTGCCAGATCACCACCTCTCTGTACCGAGCTCAGCAGATCATCCAGCTCAACATCCAAGCTTCCC; CTAAAGTACGACTGAGCTTGGCAAACGAAGCTCTGCTGCCCACCCTCATCTGCGACATTGCTGGCTATTA; CCCTCTGGATGTGGTGGTGACGTGGACCCGAGAGGAGCTGGGTGGATCCCCAGCCCAAGTCTCTGGTGCC; TCCTTCTCCAGCCTCAGGCAAAGCGTGGCAGGCACCTACAGCATCTCCTCCTCTCTCACCGCAGAACCTG; GCTCTGCAGGTGCCACTTACACCTGCCAGGTCACACACATCTCTCTGGAGGAGCCCCTTGGGGCCAGCAC; CCAGGTTGTCCCACCAGAGCGGAGAACAGCCTTGGGAGTCATCTTTGCCAGCAGTCTCTTCCTTCTTGCA; CTGATGTTCCTGGGGCTTCAGAGACGGCAAGCACCTACAGGACTTGGGCTGCTTCAGGCTGAACGCTGGG; AGACCACTTCCTGTGCTGACACACAGAGCTCCCATCTCCATGAAGACCGCACAGCGCGTGTAAGCCAGCC; CAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201899 protein sequence; Red=Cloning site Green=Tags(s); ; MGTQEGWCLLLCLALSGAAETKPHPAEGQWRAVDVVLDCFLVKDGAHRGALASSEDRARASLVLKQVPVL; DDGSLEDFTDFQGGTLAQDDPPIIFEASVDLVQIPQAEALLHADCSGKEVTCEISRYFLQMTETTVKTAA; WFMANVQVSGGGPSISLVMKTPRVAKNEVLWHPTLNLPLSPQGTVRTAVEFQVMTQTQSLSFLLGSSASL; DCGFSMAPGLDLISVEWRLQHKGRGQLVYSWTAGQGQAVRKGATLEPAQLGMARDASLTLPGLTIQDEGT; YICQITTSLYRAQQIIQLNIQASPKVRLSLANEALLPTLICDIAGYYPLDVVVTWTREELGGSPAQVSGA; SFSSLRQSVAGTYSISSSLTAEPGSAGATYTCQVTHISLEEPLGASTQVVPPERRTALGVIFASSLFLLA; LMFLGLQRRQAPTGLGLLQAERWETTSCADTQSSHLHEDRTARVSQPS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018009
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018009.3, NP_060479.2
• RefSeq Size: 1834 bp
• RefSeq ORF: 1407 bp
• Locus ID: 55080
• UniProt ID: Q9BX59
• Cytogenetics: 12p13.31
• Domains: ig, IG, IGc1
• Protein Families: Druggable Genome, Transmembrane
• MW: 50.2 kDa
• Summary: Tapasin, or TAPBP (MIM 601962), is a member of the variable-constant Ig superfamily that links major histocompatibility complex (MHC) class I molecules to the transporter associated with antigen processing (TAP; see MIM 170260) in the endoplasmic reticulum (ER). The TAPBP gene is located near the MHC complex on chromosome 6p21.3. TAPBPL is a member of the Ig superfamily that is localized on chromosome 12p13.3, a region somewhat paralogous to the MHC.[supplied by OMIM, Mar 2008]