HYPE (FICD) (NM_007076) Human Tagged ORF Clone

SKU RC201725

FICD (Myc-DDK-tagged)-Human FIC domain containing (FICD)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: HYPE
• Synonyms: HIP13; HYPE; UNQ3041
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201725 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGCTCATACCAATGGCTTCAGTGATGGCGGTGACTGAACCGAAATGGGTCTCGGTCTGGAGCCGCT; TCCTCTGGGTGACGCTGCTGAGCATGGTGCTGGGGTCCCTGCTGGCCCTGCTGCTGCCGCTGGGGGCTGT; GGAGGAGCAGTGCTTGGCTGTGCTCAAAGGCCTCTACCTGCTCAGGAGCAAACCGGACAGGGCGCAGCAT; GCCGCCACCAAGTGCACCAGCCCGTCCACGGAGCTCAGCATCACCTCCAGGGGCGCGACGCTGCTGGTGG; CCAAGACCAAGGCCTCTCCAGCGGGTAAGTTGGAAGCCAGAGCTGCCCTGAACCAGGCCCTGGAGATGAA; GCGCCAGGGCAAGCGGGAAAAAGCCCAAAAGCTCTTCATGCACGCCCTCAAGATGGACCCGGACTTCGTG; GACGCGCTCACCGAGTTTGGCATCTTCTCGGAAGAAGACAAGGACATCATCCAGGCGGACTACTTGTACA; CCAGAGCATTGACCATCTCACCCTACCATGAGAAAGCACTGGTCAACCGCGATCGGACACTGCCTCTTGT; GGAAGAGATCGACCAGAGGTATTTCAGCATCATCGACAGCAAAGTGAAGAAGGTCATGTCCATCCCCAAG; GGGAACTCAGCTCTGCGCAGGGTCATGGAGGAGACCTACTACCATCACATCTACCACACAGTGGCCATCG; AGGGCAACACCCTCACCCTCTCGGAAATCAGGCACATCCTGGAGACCCGCTACGCCGTGCCCGGGAAGAG; CCTGGAGGAGCAGAACGAGGTCATAGGCATGCATGCAGCCATGAAGTACATCAACACGACTCTGGTTTCG; CGCATCGGCTCCGTCACCATCAGCGACGTGCTGGAGATCCACAGGCGGGTGCTGGGCTACGTGGACCCCG; TGGAAGCCGGCAGGTTTCGGACAACACAGGTCCTGGTCGGACACCACATCCCTCCCCATCCGCAGGATGT; GGAAAAGCAGATGCAGGAGTTTGTACAGTGGCTCAACTCCGAGGAAGCCATGAACCTGCACCCAGTGGAG; TTTGCAGCCTTAGCCCATTATAAACTCGTTTACATCCACCCTTTCATTGATGGCAACGGGAGGACCTCCC; GTCTGCTCATGAACCTCATCCTCATGCAGGCGGGCTACCCGCCCATCACCATCCGCAAGGAGCAGCGGTC; CGACTACTACCACGTGTTGGAAGCTGCCAACGAGGGCGACGTGAGGCCTTTCATTCGCTTCATCGCCAAG; TGTACTGAGACCACCCTGGACACCCTGCTTTTTGCCACAACTGAGTACTCGGTGGCACTGCCAGAAGCCC; AACCCAACCACTCTGGGTTCAAGGAGACGCTTCCTGTGAAGCCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201725 protein sequence; Red=Cloning site Green=Tags(s); ; MMLIPMASVMAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQH; AATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFV; DALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPK; GNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVS; RIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVE; FAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAK; CTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007076
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007076.3
• RefSeq Size: 1651 bp
• RefSeq ORF: 1377 bp
• Locus ID: 11153
• UniProt ID: Q9BVA6
• Cytogenetics: 12q23.3
• Protein Families: Transmembrane
• MW: 51.8 kDa
• Summary: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).[UniProtKB/Swiss-Prot Function]