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CIB1 (NM_006384) Human Tagged ORF Clone

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SKU
RC201591
CIB1 (Myc-DDK-tagged)-Human calcium and integrin binding 1 (calmyrin) (CIB1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CIB1 or NM_006384"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CIB1
Synonyms CIB; CIBP; KIP1; PRKDCIP; SIP2-28
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201591 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGGCTCGGGCAGTCGCCTGTCCAAGGAGCTGCTGGCCGAGTACCAGGACTTGACGTTCCTGACGA
AGCAGGAGATCCTCCTAGCCCACAGGCGGTTTTGTGAGCTGCTTCCCCAGGAGCAGCGGAGCGTGGAGTC
GTCACTTCGGGCACAAGTGCCCTTCGAGCAGATTCTCAGCCTTCCAGAGCTCAAGGCCAACCCCTTCAAG
GAGCGAATCTGCAGGGTCTTCTCCACATCCCCAGCCAAAGACAGCCTTAGCTTTGAGGACTTCCTGGATC
TCCTCAGTGTGTTCAGTGACACAGCCACGCCAGACATCAAGTCCCATTATGCCTTCCGCATCTTTGACTT
TGATGATGACGGAACCTTGAACAGAGAAGACCTGAGCCGGCTGGTGAACTGCCTCACGGGAGAGGGCGAG
GACACACGGCTTAGTGCGTCTGAGATGAAGCAGCTCATCGACAACATCCTGGAGGAGTCTGACATTGACA
GGGATGGAACCATCAACCTCTCTGAGTTCCAGCACGTCATCTCCCGTTCTCCAGACTTTGCCAGCTCCTT
TAAGATTGTCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201591 protein sequence
Red=Cloning site Green=Tags(s)

MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFK
ERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGE
DTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006384
ORF Size 573 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006384.4
RefSeq Size 995 bp
RefSeq ORF 576 bp
Locus ID 10519
UniProt ID Q99828, A0A140VK09
Cytogenetics 15q26.1
Domains EFh
MW 21.7 kDa
Summary This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. The encoded protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. The encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2013
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"NM_006384" in other vectors (7)
SKU Description Size Price
RC201591L1 Lenti ORF clone of Human calcium and integrin binding 1 (calmyrin) (CIB1), Myc-DDK-tagged 10 ug
$600.00
RC201591L2 Lenti ORF clone of Human calcium and integrin binding 1 (calmyrin) (CIB1), mGFP tagged 10 ug
$600.00
RC201591L3 Lenti ORF clone of Human calcium and integrin binding 1 (calmyrin) (CIB1), Myc-DDK-tagged 10 ug
$600.00
RC201591L4 Lenti ORF clone of Human calcium and integrin binding 1 (calmyrin) (CIB1), mGFP tagged 10 ug
$600.00
RG201591 CIB1 (tGFP-tagged) - Human calcium and integrin binding 1 (calmyrin) (CIB1) 10 ug
$489.00 $500.00
SC116165 CIB1 (untagged)-Human calcium and integrin binding 1 (calmyrin) (CIB1) 10 ug
$300.00
SC322192 CIB1 (untagged)-Human calcium and integrin binding 1 (calmyrin) (CIB1) 10 ug
$300.00

Plasmid Map for RC201591

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201591
Circular map for RC201591

file-alt Citations

The human CIB1-EVER1-EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses
de Jong, Créquer, Matos et al
J Exp Med (2018) 215 (9), 2289-2310 DOI: 10.1084/jem.20170308
Cited by 133
AI Summary: In this study, OriGene products—including the CIB1 (RC208307), TMC6/EVER1 (RC217753), and TMC8/EVER2 (RC208305) expression plasmids—were used to investigate the interactions and functional roles of these proteins in keratinocyte immunity to β-papillomaviruses.
FigureFigureFigure
Showing 1-1 of 1 papers.
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