YL1 (VPS72) (NM_005997) Human Tagged Lenti ORF Clone

SKU
RC201273L3
Lenti ORF clone of Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$757.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol YL1
Synonyms CFL1; Swc2; TCFL1; YL-1; YL1
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC201273).
Restriction Sites SgfI-MluI Cloning Scheme for this gene
ACCN NM_005997
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005997.1
RefSeq Size 1546 bp
RefSeq ORF 1095 bp
Locus ID 6944
UniProt ID Q15906
Cytogenetics 1q21.3
Protein Families Transcription Factors
MW 40.6 kDa
Summary The protein encoded by this gene is a shared subunit of two multi-component complexes, the histone acetyltransferase complex TRRAP/TIP60 as well as the chromatin remodeling SRCAP-containing complex. The TRRAP/TIP60 complex acetylates nucleosomal histones important for transcriptional regulation, double strand DNA break repair and apoptosis. The SRCAP-containing complex catalyzes the exchange of histone H2A with the histone variant Htz1 (H2AFZ) into nucleosomes. This protein may be responsible for binding H2AFZ, which has a role in chromosome segregation. This protein may also have a role in regulating long-term hematopoietic stem cell activity. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]
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SKU Description Size Price
RC201273 VPS72 (Myc-DDK-tagged)-Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72) 10 ug
$457.00
RC201273L1 Lenti ORF clone of Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72), Myc-DDK-tagged 10 ug
$757.00
RC201273L2 Lenti ORF clone of Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72), mGFP tagged 10 ug
$757.00
RC201273L4 Lenti ORF clone of Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72), mGFP tagged 10 ug
$757.00
RG201273 VPS72 (tGFP-tagged) - Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72) 10 ug
$657.00
SC320034 VPS72 (untagged)-Human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72) 10 ug
$457.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.