Perilipin 3 (PLIN3) (NM_005817) Human Tagged ORF Clone

SKU RC201193

PLIN3 (Myc-DDK-tagged)-Human perilipin 3 (PLIN3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Perilipin 3
• Synonyms: M6PRBP1; PP17; TIP47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201193 representing NM_005817; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTGCCGACGGGGCAGAGGCTGATGGCAGCACCCAGGTGACAGTGGAAGAACCGGTACAGCAGCCCA; GTGTGGTGGACCGTGTGGCCAGCATGCCTCTGATCAGCTCCACCTGCGACATGGTGTCCGCAGCCTATGC; CTCCACCAAGGAGAGCTACCCGCACATCAAGACTGTCTGCGACGCAGCAGAGAAGGGAGTGAGGACCCTC; ACGGCGGCTGCTGTCAGCGGGGCTCAGCCGATCCTCTCCAAGCTGGAGCCCCAGATTGCATCAGCCAGCG; AATACGCCCACAGGGGGCTGGACAAGTTGGAGGAGAACCTCCCCATCCTGCAGCAGCCCACGGAGAAGGT; CCTGGCGGACACCAAGGAGCTTGTGTCGTCTAAGGTGTCGGGGGCCCAAGAGATGGTGTCTAGCGCCAAG; GACACGGTGGCCACCCAATTGTCGGAGGCGGTGGACGCGACCCGCGGTGCTGTGCAGAGCGGCGTGGACA; AGACAAAGTCCGTAGTGACCGGCGGCGTCCAATCAGTCATGGGCTCCCGCTTGGGCCAGATGGTGCTGAG; TGGGGTCGACACGGTGCTGGGGAAGTCGGAGGAGTGGGCGGACAACCACCTGCCCCTTACGGATGCCGAA; CTGGCCCGCATCGCCACATCCCTGGATGGCTTCGACGTCGCGTCCGTGCAGCAGCAGCGGCAGGAACAGA; GCTACTTCGTACGTCTGGGCTCCCTGTCGGAGAGGCTGCGGCAGCACGCCTATGAGCACTCGCTGGGCAA; GCTTCGAGCCACCAAGCAGAGGGCACAGGAGGCTCTGCTGCAGCTGTCGCAGGCCCTAAGCCTGATGGAA; ACTGTCAAGCAAGGCGTTGATCAGAAGCTGGTGGAAGGCCAGGAGAAGCTGCACCAGATGTGGCTCAGCT; GGAACCAGAAGCAGCTCCAGGGCCCCGAGAAGGAGCCGCCCAAGCCAGAGCAGGTCGAGTCCCGGGCGCT; CACCATGTTCCGGGACATTGCCCAGCAACTGCAGGCCACCTGTACCTCCCTGGGGTCCAGCATTCAGGGC; CTCCCCACCAATGTGAAGGACCAGGTGCAGCAGGCCCGCCGCCAGGTGGAGGACCTCCAGGCCACGTTTT; CCAGCATCCACTCCTTCCAGGACCTGTCCAGCAGCATTCTGGCCCAGAGCCGTGAGCGTGTCGCCAGCGC; CCGCGAGGCCCTGGACCACATGGTGGAATATGTGGCCCAGAACACACCTGTCACGTGGCTCGTGGGACCC; TTTGCCCCTGGAATCACTGAGAAAGCCCCGGAGGAGAAGAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201193 representing NM_005817; Red=Cloning site Green=Tags(s); ; MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTL; TAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAK; DTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAE; LARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQALSLME; TVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQG; LPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGP; FAPGITEKAPEEKK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005817
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005817.2
• RefSeq Size: 2241 bp
• RefSeq ORF: 1305 bp
• Locus ID: 10226
• UniProt ID: O60664
• Cytogenetics: 19p13.3
• Domains: perilipin
• Protein Families: Druggable Genome
• MW: 46.9 kDa
• Summary: Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]