A RAF(ARAF) (NM_001654) Human Tagged ORF Clone

CAT#: RC200737

ARAF (Myc-DDK-tagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)

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Size
    • 10 ug

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Specifications

Product Data
Product Name A RAF(ARAF) (NM_001654) Human Tagged ORF Clone
Symbol ARAF
Synonyms A-RAF; ARAF1; PKS2; RAFA1
Vector pCMV6-Entry
Sequence Data
>RC200737 representing NM_001654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACCGTCAAAGTAT
ACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAA
GGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGGACGAAAGACG
GTCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATG
TCCCGCTGACCATGCACAATTTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAA
GTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAG
GCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCAGGGTCCCAGCCC
CCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATC
CGCTCCACGTCCACTCCCAACGTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGC
TCACTGGCCAGAGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAG
CCCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGG
AGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCGTTTGGCACCGTGTTTCGAGG
GCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCT
TTCAAGAATGAGATGCAGGTGCTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGA
CCCGGCCGGGATTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGC
CGACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGGCTCACGGTGA
AGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCCCAGCCCTTGGAGCAGCCCTC
AGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCA
GACGTCTATGCCTACGGGGTTGTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCT
GCCGTGACCAGATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAA
CTGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGTGCCTCGGAAC
CCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGCGCAGCCCGCCTTGTGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200737 representing NM_001654
Red=Cloning site Green=Tags(s)

MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT
VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV
PTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRI
RSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRE
RKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQA
FKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYL
HAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQS
DVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLF
PQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001654
ORF Size 1818 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_001654.1, NM_001654.2, NM_001654.3, NM_001654.4, NP_001645
RefSeq Size 2466
RefSeq ORF 1821
Locus ID 369
Cytogenetics Xp11.3
Domains pkinase, TyrKc, C1, S_TKc, RBD
Protein Families Protein Kinase, Druggable Genome
Protein Pathways ErbB signaling pathway, Vascular smooth muscle contraction, Natural killer cell mediated cytotoxicity, Long-term potentiation, Long-term depression, Regulation of actin cytoskeleton, Insulin signaling pathway, Progesterone-mediated oocyte maturation, Pathways in cancer, Colorectal cancer, Renal cell carcinoma, Pancreatic cancer, Endometrial cancer, Glioma, Prostate cancer, Melanoma, Bladder cancer, Chronic myeloid leukemia, Acute myeloid leukemia, Non-small cell lung cancer
MW 67.4 kDa
Gene Summary This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012].
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Citations (1)