A RAF (ARAF) (NM_001654) Human Tagged ORF Clone

CAT#: RC200737

ARAF (Myc-DDK-tagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARAF (Cat# RC200737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF(Cat# TA803663). Positive lysates LY400623 (100ug) and LC400623 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400623) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200737 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARAF protein (Cat# TP300737). The protein was produced from HEK293T cells transfected with ARAF cDNA clone (Cat# RC200737) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARAF
• Synonyms: A-RAF; ARAF1; PKS2; RAFA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200737 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACCGTCAAAGTAT
  ACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAA
  GGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGGACGAAAGACG
  GTCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATG
  TCCCGCTGACCATGCACAATTTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAA
  GTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAG
  GCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCAGGGTCCCAGCCC
  CCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATC
  CGCTCCACGTCCACTCCCAACGTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGC
  TCACTGGCCAGAGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAG
  CCCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
  CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGG
  AGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCGTTTGGCACCGTGTTTCGAGG
  GCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCT
  TTCAAGAATGAGATGCAGGTGCTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGA
  CCCGGCCGGGATTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGC
  CGACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
  CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGGCTCACGGTGA
  AGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCCCAGCCCTTGGAGCAGCCCTC
  AGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCA
  GACGTCTATGCCTACGGGGTTGTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCT
  GCCGTGACCAGATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAA
  CTGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
  CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGTGCCTCGGAAC
  CCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGCGCAGCCCGCCTTGTGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200737 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT
  VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV
  PTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRI
  RSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRE
  RKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQA
  FKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYL
  HAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQS
  DVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLF
  PQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001654
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001654.5
• RefSeq Size: 2562 bp
• RefSeq ORF: 1821 bp
• Locus ID: 369
• UniProt ID: P10398
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
• MW: 67.6 kDa
• Gene Summary: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]