A RAF (ARAF) (NM_001654) Human Untagged Clone

CAT#: SC119127

ARAF (untagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: A RAF
• Synonyms: A-RAF; ARAF1; PKS2; RAFA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001654 edited
  ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
  GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
  GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
  GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
  CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
  TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
  CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGAT
  TTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTA
  ACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT
  GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGTCCATATG
  GTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAGTTTCAGC
  ACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCCCAGCCCA
  GCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
  CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC
  TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCG
  TTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTG
  TCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCTCAGGAAG
  ACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATC
  ATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC
  TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
  CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGG
  CTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCC
  CAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATG
  CAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC
  GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGATTATCTTT
  ATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTGCCCCAAG
  GCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
  CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGT
  GCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC
  GCAGCCCGCCTTGTGCCTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001654 unedited
  GGTTTACAATTGTAAACGACTCATATAGGCGGCCGCGAAATTCGCACGAAGGCCAAAATG
  GAGACGGCGGCGGCTGTAGCGGCGTGACAGGAGCCCCATGGCACCTGCCCAGCCCCACCT
  CAGCCCATCTTGACAAAATCTAAGGCTCCATGGAGCCACCACGGGGCCCCCCTGCCAATG
  GGGCCGAGCCATCCCGGGCAGTGGGCACCGTCAAAGTATACCTGCCCAACAAGCAACGCA
  CGGTGGTGACTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAAGGCCCTGAAGG
  TGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGGACGAAAGACGG
  TCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCGAGGAGCTCATTGTCGAGGTCC
  TTGAAGATGTCCCGCTGACCATGCACAATTTTGTACGGAAGACCTTCTTCAGCCTGGCGT
  TCTGTGACTTCTGCCTTAAGTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACA
  AGTTCCACCAGCATTGTTCCTCCAAGGTCCCCACAGTCTGTGTTGACATGAGTACCAACC
  GCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTC
  CCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCCAGGTCCCAGNCCCCGCACCCAGC
  ACTGTGACCCGNAGCACTTNCCCCTTCCTGCCCCAACCAATGCCCCCCCTACAGCGCATC
  CGCTCCACGTCCACTCCCCACGTCCATATGGTCAACACCACGGCCCCCATGGACTCCAAC
  TCATNCAGCTCACTGGCCAAAGTTTCANCACTGATGCTNGCCGTAGTANAAGAAGTANTG
  ATGGAAACCCCCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001654 unedited
  CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGCAGAATTCTGTGCTTC
  TTGGTGAAGAAAGAGAGGAGTCCTTGAATGGCGGGGGAGACACAAAAGGGACCCCCCAAA
  ATCCACATGGGGTGCTGGGAACCCCAAAATCCAGTGGAAGGCACATCAGGCACAAATTCC
  AGAGAGGTTCTGAGTGGAATCCCTGCCACAAATCCCAGCCATTGGAGATGGAGGAGCTCC
  CAAAATTTAAAAGTATCCCCAAAGCCAAGAGGAAACCAAATGATGGAGGAGACAGGGGGC
  TCAGTCTTTGGCGGGGGTCCCCCAATTCCAGAAGAACTGGAAAAGCACATGGGGACCCCC
  TCATCTCCCAGGGTGGGGAATGGGGGATCCTGAGGCAGCATCAGGGCAGAGACGAACATT
  GATTGGCTGGTGGGCAAGGCTCCTTGGCGTGGAGGGCTGAAATTGGCTCCCTGGTGGCTT
  GGGCGGGGCCTAAGGCACAAGGCGGGCTGCGCTGAGTAGGCAGGCAGGCAACTCATCGGC
  CTGNGTGCGGTGCAAGGAGGGTTCCGAGGCACTCCGCTCAATCTTGGGGAGTGACCGTTG
  CAGCAGCTCAATTGTGGCCAGGATCTGGGGGAAGAGGGGCCGCTCCTCCCGCTGGAACTT
  GAGGCANTCANACAGCANGCGCCGCATGGCCTTGGGGCANNTGCTGGAGATTTTGCCTGA
  GTCGGGGACAGATACCCCGGCCCACATAAAGATATCTGGTCACGCACCCATGTGGCTGTA
  GGCANTGAGCCANTCATACCTCCTANACCCACCCCCGTAGCTANACTCTGACTGGAACTT
  AAGGGGTCCGGTCCTGCTTACGATCACCTANCTGCCTCCCAGCAAAATCTGGAGGCTGCT
  CAAGGCTGGCCCCCCCTCTTGAGTCTCCTGTGCCAACAAAAACACGTCTTACCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001654
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001654.1, NP_001645.1
• RefSeq Size: 2466 bp
• RefSeq ORF: 1821 bp
• Locus ID: 369
• UniProt ID: P10398
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
• Gene Summary: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) encodes the predominant isoform (1).