A RAF (ARAF) (NM_001654) Human Untagged Clone

CAT#: SC323512

ARAF (untagged)-Kinase deficient mutant (K336M) of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: A RAF
• Synonyms: A-RAF; ARAF1; PKS2; RAFA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323512 sequence for NM_001654 edited (data generated by NextGen Sequencing)
  ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
  GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
  GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
  GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
  CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
  TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
  CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGAT
  TTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTA
  ACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT
  GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGTCCATATG
  GTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAGTTTCAGC
  ACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCCCAGCCCA
  GCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
  CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC
  TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCG
  TTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGATGGTGCTCAAGGTG
  TCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCTCAGGAAG
  ACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATC
  ATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC
  TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
  CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGG
  CTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCC
  CAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATG
  CAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC
  GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGATTATCTTT
  ATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTGCCCCAAG
  GCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
  CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGT
  GCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC
  GCAGCCCGCCTTGTGCCTTAG
  
  Clone variation with respect to NM_001654.3
  1007 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001654 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAAGATGGAGACG
  GCGGCGGCTGTAGCGGCGTGACAGGAGCCCCATGGCACCTGCCCAGCCCCACCTCAGCCCATCTTGACAA
  AATCTAAGGCTCCATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGC
  ACCGTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCCGGGATGGCATGAGTGTCTAC
  GACTCTCTAGACAAGGCCCCTGAAGGTGCGGGGGTCTAAATCAGGACTGCTGTGGTGGTCTACCGACTCA
  TCAAGGGACGAAAGACGGTCACTGGCCTGGGACCACAGCAATTGGCTCCCCCTGAATGGCAAGGGAGCTC
  ATGGTCAAGGTCCTTGAGAATGTCCGGCTAACCTTGCACAATTTTTGTCCGGGAGAACCTTCTTAGCCCT
  GGCTTCCGTGGACTTTGGCCTAAAGTTCCGGTTCCTGGCTTTCCGTTGCCAACTGGGGTCCAAATTCCCC
  CACACTTTGTCCCTCAAGTCCCACAGTCTGTGTGTACATGAGATCACACGCCACACATTCTACCCACAGT
  GCACAATATTGCCGAGAGTCACACACATGAGCGTCCTCTGAACCCCCTGATGAGATGTGTACACCCAGGT
  GTCACGTCCGACAGACCTGTGAACGGGACATTCCTTCTGGCAGCAATGGCCCTAGGGATACGTTCAGTCA
  TTCCAAGTATTGTAGCACGGCAATGATCACATCACGTCTTGCCAGATTACTAGTCCGATAGGAGCGTGTG
  ACAGGATCGCAACGG
  
  Kinase domain sequence

  >SC323512 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AKCRGTGCMGCTGCTGAGAGGWCGGGACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGA
  TGTGGCCGTGATGGTGCTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATG
  CAGGTGCTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTG
  CCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACC
  
• Restriction Sites: Please inquire
• ACCN: NM_001654
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001654.1, NP_001645.1
• RefSeq Size: 2466 bp
• RefSeq ORF: 1821 bp
• Locus ID: 369
• UniProt ID: P10398
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
• Gene Summary: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) encodes the predominant isoform (1).