Calpain 6 (CAPN6) (NM_014289) Human Tagged ORF Clone

SKU RC200539

CAPN6 (Myc-DDK-tagged)-Human calpain 6 (CAPN6)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Calpain 6
• Synonyms: CalpM; CANPX; CAPNX; DJ914P14.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200539 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGCATCAAAGACA; GCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTCTACAACCGACTGCTTCCTGG; AAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGACCCCCATCTGATTGTGGGCAACATTAGCAAC; CACCAGCTGACCCAAGGGAGACTGGGGCACAAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGG; AGTCTCATTGGACAAAGACAATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATA; CGCTGGGATATTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG; CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAATGCTCTGCTGG; AAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTGACCATCACTGATATTATTGT; GGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAGAAAGGAAGATACACTGAGCTTGTTGAGGAG; AAGTACAAGCTATTCGGAGAACTGTACAAAACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGT; CTCCCAATCAGGAGGAGCAAGAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGAC; TGATATTCGCAAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT; CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAAGAGTGGCAGC; AACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGATGGAGAGTTTTGGATGAGCTT; GGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGCCGCAATGTGAACAACCCTATTTTTGGCCGA; AAGGAGCTGGAATCGGTGTTGGGATGCTGGACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCT; GCTATAACAACCGTGATACCTTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAA; GGTCATTATGTCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC; ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTACATCCAGGAGC; GTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAGTACCTGAAGAAGGGCAACTA; TGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGCGAGTTTCTCCTGAGAATCTTCTCTGAAGTG; CCTGTCCAGCTCAGGGAACTGACTCTGGACATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACC; CGAAAGTAGTTACTCAGATCACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGT; AAACCCATATTTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT; GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTACAGGTCTGGA; ACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCTGACCCCAGCGACTGCCGTGA; TCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACTGCCAAAGTCAAGCAAGGCCACATCAGCTTC; AAGGTTATTTCCAGCGATGATCTCACTGAGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200539 protein sequence; Red=Cloning site Green=Tags(s); ; MGPPLKLFKNQKYQELKQECIKDSRLFCDPTFLPENDSLFYNRLLPGKVVWKRPQDICDDPHLIVGNISN; HQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLL; PTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEE; KYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMV; RLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGR; KELESVLGCWTVDDDPLMNRSGGCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGRPDNYI; IGFELFKVEMNRKFRLHHLYIQERAGTSTYIDTRTVFLSKYLKKGNYVLVPTMFQHGRTSEFLLRIFSEV; PVQLRELTLDMPKMSCWNLARGYPKVVTQITVHSAEDLEKKYANETVNPYLVIKCGKEEVRSPVQKNTVH; AIFDTQAIFYRRTTDIPIIVQVWNSRKFCDQFLGQVTLDADPSDCRDLKSLYLRKKGGPTAKVKQGHISF; KVISSDDLTEL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014289
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014289.4
• RefSeq Size: 3604 bp
• RefSeq ORF: 1926 bp
• Locus ID: 827
• UniProt ID: Q9Y6Q1
• Cytogenetics: Xq23
• Domains: C2, Calpain_III
• Protein Families: Druggable Genome, Protease
• MW: 74.6 kDa
• Summary: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]