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RPL38 (NM_001035258) Human Tagged ORF Clone

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SKU
RC200423
RPL38 (Myc-DDK-tagged)-Human ribosomal protein L38 (RPL38), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPL38
Synonyms L38
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200423 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCGGAAAATTGAGGAAATCAAGGACTTCCTGCTCACAGCCCGACGAAAGGATGCCAAATCTGTCA
AGATCAAGAAAAATAAGGACAACGTGAAGTTTAAAGTTCGATGCAGCAGATACCTTTACACCCTGGTCAT
CACTGACAAAGAGAAGGCAGAGAAACTGAAGCAGTCCCTGCCCCCCGGTTTGGCAGTGAAGGAACTGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200423 protein sequence
Red=Cloning site Green=Tags(s)

MPRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLPPGLAVKELK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001035258
ORF Size 210 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001035258.2
RefSeq Size 376 bp
RefSeq ORF 213 bp
Locus ID 6169
UniProt ID P63173, A0A024R8P8
Cytogenetics 17q25.1
Protein Families Druggable Genome
Protein Pathways Ribosome
MW 8.2 kDa
Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L38E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants have been identified, both encoding the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome, including one located in the promoter region of the type 1 angiotensin II receptor gene. provided by RefSeq, Jul 2008
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"NM_001035258" in other vectors (7)
SKU Description Size Price
RC200423L1 Lenti ORF clone of Human ribosomal protein L38 (RPL38), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC200423L2 Lenti ORF clone of Human ribosomal protein L38 (RPL38), transcript variant 2, mGFP tagged 10 ug
$450.00
RC200423L3 Lenti ORF clone of Human ribosomal protein L38 (RPL38), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC200423L4 Lenti ORF clone of Human ribosomal protein L38 (RPL38), transcript variant 2, mGFP tagged 10 ug
$450.00
RG200423 RPL38 (tGFP-tagged) - Human ribosomal protein L38 (RPL38), transcript variant 2 10 ug
$489.00
SC302831 RPL38 (untagged)-Human ribosomal protein L38 (RPL38), transcript variant 2 10 ug
$165.00
SC320632 RPL38 (untagged)-Human ribosomal protein L38 (RPL38), transcript variant 2 10 ug
$150.00

Plasmid Map for RC200423

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200423
Circular map for RC200423

file-alt Citations

Ectopic mineralization in the middle ear and chronic otitis media with effusion caused by RPL38 deficiency in the Tail-short (Ts) mouse
Noben-Trauth, Latoche
J. Biol. Chem. (2011) 286 (4), 3079-93 DOI: 10.1074/jbc.M110.184598
Cited by 5
AI Summary: OriGene contributed to this study by providing the Anti-Myc antibody and Myc-tagged human Rpl38 (NP_001030335) protein lysates, which were used for immunohistochemistry and protein expression analyses to investigate the role of RPL38 in middle ear pathology.
Snippet: Anti-Myc antibody was obtained from OriGene. Histology and Immunohistochemistry. For ... Rad). Protein lysates of HEK293T cells transfected with and without Myc-tagged human Rpl38 (NP_001030335) were obtained from OriGene.
IHC WB
Showing 1-1 of 1 papers.
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