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ECHS1 (NM_004092) Human Tagged ORF Clone

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SKU
RC200369
ECHS1 (Myc-DDK-tagged)-Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ECHS1 or NM_004092"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ECHS1
Synonyms ECHS1D; SCEH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200369 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCCTGCGTGTCCTGCTGTCCTGCGTCCGCGGCCCGCTGAGGCCCCCGGTTCGCTGTCCCGCCT
GGCGTCCCTTCGCCTCGGGTGCTAACTTTGAGTACATCATCGCAGAAAAAAGAGGGAAGAATAACACCGT
GGGGTTGATCCAACTGAACCGCCCCAAGGCCCTCAATGCACTTTGCGATGGCCTGATTGACGAGCTCAAC
CAGGCCCTGAAGATCTTCGAGGAGGACCCGGCCGTGGGGGCCATTGTCCTCACCGGCGGGGATAAGGCCT
TTGCAGCTGGAGCTGATATCAAGGAAATGCAGAACCTGAGTTTCCAGGACTGTTACTCCAGCAAGTTCTT
GAAGCACTGGGACCACCTCACCCAGGTCAAGAAGCCAGTCATCGCTGCTGTCAATGGCTATGCCTTTGGC
GGGGGCTGTGAGCTTGCCATGATGTGTGATATCATCTATGCCGGTGAGAAGGCCCAGTTTGCACAGCCGG
AGATCTTAATAGGAACCATCCCAGGTGCGGGCGGCACCCAGAGACTCACCCGTGCTGTTGGGAAGTCGCT
GGCGATGGAGATGGTCCTCACCGGTGACCGGATCTCAGCCCAGGACGCCAAGCAAGCAGGTCTTGTCAGC
AAGATTTGTCCTGTTGAGACACTGGTGGAAGAAGCCATCCAGTGTGCAGAAAAAATTGCCAGCAATTCTA
AAATTGTAGTAGCGATGGCCAAAGAATCAGTGAATGCAGCTTTTGAAATGACATTAACAGAAGGAAGTAA
GTTGGAGAAGAAACTCTTTTATTCAACCTTTGCCACTGATGACCGGAAAGAAGGGATGACCGCGTTTGTG
GAAAAGAGAAAGGCCAACTTCAAAGACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200369 protein sequence
Red=Cloning site Green=Tags(s)

MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELN
QALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFG
GGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
KICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFV
EKRKANFKDQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004092
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004092.4
RefSeq Size 1350 bp
RefSeq ORF 873 bp
Locus ID 1892
UniProt ID P30084
Cytogenetics 10q26.3
Domains ECH
Protein Pathways beta-Alanine metabolism, Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, leucine and isoleucine degradation, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Tryptophan metabolism, Valine
MW 31.4 kDa
Summary The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. provided by RefSeq, Jul 2008
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"NM_004092" in other vectors (6)
SKU Description Size Price
RC200369L1 Lenti ORF clone of Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC200369L2 Lenti ORF clone of Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RC200369L3 Lenti ORF clone of Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC200369L4 Lenti ORF clone of Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG200369 ECHS1 (tGFP-tagged) - Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein 10 ug
$500.00
SC319501 ECHS1 (untagged)-Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein 10 ug
$300.00

Plasmid Map for RC200369

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200369
Circular map for RC200369

file-alt Citations

Development of iPSC-based clinical trial selection platform for patients with ultrarare diseases
Sequiera, Srivastava, Sareen et al
Sci Adv (2022) 8 (14), eabl4370 DOI: 10.1126/sciadv.abl4370
Cited by 31
AI Summary: OriGene’s ECHS1 expression vector (OriGene no. RC200369) was used to ectopically express wild-type ECHS1 protein in patient-derived iPSC fibroblasts, enabling functional studies critical to the development of the personalized drug screening platform described in the paper.
Snippet: … ECHS1 expression in cells WT ECHS1 protein was ectopically expressed in iPSC-derived fibroblasts of ECHS1 patient using an expression vector (OriGene no. RC200369). …The transfected fibroblast;- WT ECHS1 protein was ectopically expressed in iPSC-derived fibroblasts of ECHS1 patient using an expression vector (OriGene no. RC200369). …
FigureFigureFigure
Inactivation of the AMPK-GATA3-ECHS1 Pathway induces Fatty Acid Synthesis that Promotes Clear Cell Renal Cell Carcinoma Growth
Qu, Zhao, Zhang et al
Cancer Res. (2019)
AI Summary: OriGene contributed to this study by providing whole-length ECHS1 cDNA clones, which were used to investigate the role of ECHS1 in fatty acid metabolism and clear cell renal cell carcinoma growth.
Snippet: Whole-length ECHS1 cDNA clones were purchased from OriGene (Rockville, MD, 6 USA). After confirming the sequence by Sanger sequencing, ECHS1 was cloned into 7 the pcDNA3.1-Flag vector (Invitrogen). The ECHS1 promoter reporter plasmid, 8 containing the 1221-bp ECHS1 core promoter region fragment, was synthesized by
Showing 1-2 of 2 papers.
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