NUDT5 (NM_014142) Human Tagged ORF Clone

SKU RC200204

NUDT5 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: NUDT5
• Synonyms: hNUDT5; YSA1; YSA1H; YSAH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200204 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAGCCAAGAACCAACGGAATCTTCTCAGAATGGCAAACAGTATATCATTTCAGAGGAGTTAATTT; CAGAAGGAAAATGGGTCAAGCTTGAAAAAACAACGTACATGGATCCTACTGGTAAAACTAGAACTTGGGA; ATCAGTGAAACGTACAACCAGGAAAGAGCAGACTGCGGATGGTGTCGCGGTCATCCCCGTGCTGCAGAGA; ACACTTCACTATGAGTGTATCGTTCTGGTGAAACAGTTCCGACCACCAATGGGGGGCTACTGCATAGAGT; TCCCTGCAGGTCTCATAGATGATGGTGAAACCCCAGAAGCAGCTGCTCTCCGGGAGCTTGAAGAAGAAAC; TGGCTACAAAGGGGACATTGCCGAATGTTCTCCAGCGGTCTGTATGGACCCAGGCTTGTCAAACTGTACT; ATACACATCGTGACAGTCACCATTAACGGAGATGATGCCGAAAACGCAAGGCCGAAGCCAAAGCCAGGGG; ATGGAGAGTTTGTGGAAGTCATTTCTTTACCCAAGAATGACCTGCTGCAGAGACTTGATGCTCTGGTAGC; TGAAGAACATCTCACAGTGGACGCCAGGGTCTATTCCTACGCTCTAGCACTGAAACATGCAAATGCAAAG; CCATTTGAAGTGCCCTTCTTGAAATTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200204 protein sequence; Red=Cloning site Green=Tags(s); ; MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQR; TLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCT; IHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAK; PFEVPFLKF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014142
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014142.4
• RefSeq Size: 1224 bp
• RefSeq ORF: 660 bp
• Locus ID: 11164
• UniProt ID: Q9UKK9
• Cytogenetics: 10p14
• Domains: NUDIX
• Protein Pathways: Purine metabolism
• MW: 24.3 kDa
• Summary: This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]