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ATF5 (NM_012068) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200081
ATF5 (Myc-DDK-tagged)-Human activating transcription factor 5 (ATF5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ATF5 or NM_012068"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATF5
Synonyms ATFX; HMFN0395
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200081 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACTCCTGGCGACCCTGGGGCTGGAGCTGGACAGGGCCCTGCTCCCAGCTAGTGGGCTGGGATGGC
TCGTAGACTATGGGAAACTCCCCCCGGCCCCTGCCCCCCTGGCTCCCTATGAGGTCCTTGGGGGAGCCCT
GGAGGGCGGGCTTCCAGTGGGGGGAGAGCCCCTGGCAGGTGATGGCTTCTCTGACTGGATGACTGAGCGA
GTTGATTTCACAGCTCTCCTCCCTCTGGAGCCTCCCCTACCCCCCGGCACCCTCCCCCAACCTTCCCCAA
CCCCACCTGACCTGGAAGCTATGGCCTCCCTCCTCAAGAAGGAGCTGGAACAGATGGAAGACTTCTTCCT
AGATGCCCCGCTCCTCCCACCACCCTCCCCGCCGCCACTACCACCACCACCACTACCACCAGCCCCCTCC
CTCCCCCTGTCCCTCCCCTCCTTTGACCTCCCCCAGCCCCCTGTCTTGGATACTCTGGACTTGCTGGCCA
TCTACTGCCGCAACGAGGCCGGGCAGGAGGAAGTGGGGATGCCGCCTCTGCCCCCGCCACAGCAGCCCCC
TCCTCCTTCTCCACCTCAACCTTCTCGCCTGGCCCCCTACCCACATCCTGCCACCACCCGAGGGGACCGC
AAGCAAAAGAAGAGAGACCAGAACAAGTCGGCGGCTCTGAGGTACCGCCAGCGGAAGCGGGCAGAGGGTG
AGGCCCTGGAGGGCGAGTGCCAGGGGCTGGAGGCACGGAATCGCGAGCTGAAGGAACGGGCAGAGTCCGT
GGAGCGCGAGATCCAGTACGTCAAGGACCTGCTCATCGAGGTTTACAAGGCCCGGAGCCAGAGGACCCGT
AGCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200081 protein sequence
Red=Cloning site Green=Tags(s)

MSLLATLGLELDRALLPASGLGWLVDYGKLPPAPAPLAPYEVLGGALEGGLPVGGEPLAGDGFSDWMTER
VDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPLPPPPLPPAPS
LPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDR
KQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTR
SC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012068
ORF Size 846 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012068.5
RefSeq Size 2293 bp
RefSeq ORF 849 bp
Locus ID 22809
UniProt ID Q9Y2D1
Cytogenetics 19q13.33
Domains BRLZ
Protein Families Transcription Factors
MW 30.7 kDa
Summary Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), ATF5-specific response element (ARE) (consensus: 5'-CCTTCTCTCCTTAT-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity). Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_012068" in other vectors (6)
SKU Description Size Price
RC200081L1 Lenti ORF clone of Human activating transcription factor 5 (ATF5), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200081L2 Lenti ORF clone of Human activating transcription factor 5 (ATF5), transcript variant 1, mGFP tagged 10 ug
$600.00
RC200081L3 Lenti ORF clone of Human activating transcription factor 5 (ATF5), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200081L4 Lenti ORF clone of Human activating transcription factor 5 (ATF5), transcript variant 1, mGFP tagged 10 ug
$600.00
RG200081 ATF5 (tGFP-tagged) - Human activating transcription factor 5 (ATF5), transcript variant 1 10 ug
$489.00 $500.00
SC319384 ATF5 (untagged)-Human activating transcription factor 5 (ATF5), transcript variant 1 10 ug
$300.00

Plasmid Map for RC200081

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200081
Circular map for RC200081

file-alt Citations

UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease
Hu, Liu, Qi
Biochem Biophys Res Commun (2021) 569, 17-22 DOI: 10.1016/j.bbrc.2021.06.079
Cited by 20
AI Summary: In this study, OriGene’s human ATF5 cDNA clone (Cat# RC200774) was used to overexpress activating transcription factor 5, enabling the authors to demonstrate that ATF5-mediated UPRmt activation protects SH-SY5Y cells from MPP+-induced mitochondrial dysfunction and cell death.
FigureFigure
UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease
Hu, Liu, Qi
Biochemical and Biophysical Research Communications (2021) 569, 17-22 DOI: 10.1016/j.bbrc.2021.06.079
Cited by 20
AI Summary: In this study, the Myc-tagged full-length human ATF5 plasmid from OriGene (catalog no. RC200081) was used to overexpress ATF5 in SH-SY5Y cells, enabling the authors to investigate the protective effects of UPRmt activation against MPP+-induced mitochondrial dysfunction and cell death.
Snippet: 2-phenylindole (DAPI). 2.8. Constructs and transfection. Δ-OTC plasmid was obtained from Addgene (catalog no. 71878). Myc-tagged full-length human ATF5 was purchased from Origene (catalog no. RC200081). Cells were;D-OTC plasmid was obtained from Addgene (catalog no. 71878).Myc-tagged full-length human ATF5 was purchased from Origene(catalog no. RC200081)
Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids
Velazquez, LeGraw, Moghadam et al
Cell Syst (2020) 12 (1), 41-55.e11 DOI: 10.1016/j.cels.2020.11.002
Cited by 108
AI Summary: OriGene’s PROX1 transcript variant 2 cDNA (Cat# RC200081) was used to overexpress PROX1 in pluripotent stem cell-derived liver organoids, enabling the researchers to reprogram gene regulatory networks and enhance organoid maturation and vascularization.
Snippet: PROX1 transcript variant 2 cDNA Origene Cat# RC200081
Showing 1-3 of 3 papers.
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