EXOSC3 (NM_016042) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EXOSC3 |
| Synonyms | bA3J10.7; CGI-102; hRrp-40; p10; PCH1B; RRP40; Rrp40p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016042 |
| ORF Size | 825 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016042.4 |
| RefSeq Size | 1857 bp |
| RefSeq ORF | 828 bp |
| Locus ID | 51010 |
| UniProt ID | Q9NQT5 |
| Cytogenetics | 9p13.2 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | RNA degradation |
| MW | 29.6 kDa |
| Summary | This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jun 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016042" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200035L3 | Lenti ORF clone of Human exosome component 3 (EXOSC3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200035L4 | Lenti ORF clone of Human exosome component 3 (EXOSC3), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG200035 | EXOSC3 (tGFP-tagged) - Human exosome component 3 (EXOSC3), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC321585 | EXOSC3 (untagged)-Human exosome component 3 (EXOSC3), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
The RNA exosome nuclease complex regulates human embryonic stem cell differentiation
Belair, Sim, Kim et al
J. Cell Biol. (2019) 218 (8), 2564-2582 DOI: 10.1083/jcb.201811148
Belair, Sim, Kim et al
J. Cell Biol. (2019) 218 (8), 2564-2582 DOI: 10.1083/jcb.201811148
AI Summary: OriGene’s pCMV6-EXOSC3 (RC200035) plasmid was used as the source of EXOSC3 cDNA to generate an shRNA-resistant transgene for functional studies of the RNA exosome in human embryonic stem cell differentiation.
Snippet: To express the shEXOSC3 RNA-resistant transgene, EXOSC3 cDNA was amplified from the plasmid pCMV6-EXOSC3 (RC200035;ORIGENE) with oligonucleotides EXOSC3-MluIF and EXOSC3-MluIR (Table S2), digested with MluI, and cloned into the piggyBac vector used to overexpress FOXH1. Silent mutations were introduced using the Q5 site-directed mutagenesis kit (New England Biolabs) using oligonucleotides EXOSC3-mut-F and EXOSC3-mut-R (Table S2)
Showing 1-1 of 1 papers.
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