Antizyme inhibitor 1 (AZIN1) (NM_015878) Human Tagged ORF Clone

SKU RC200022

AZIN1 (Myc-DDK-tagged)-Human antizyme inhibitor 1 (AZIN1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Antizyme inhibitor 1
• Synonyms: AZI; AZI1; AZIA1; OAZI; OAZIN; ODC1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200022 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAAGGATTTATTGATGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG; TTATTGATAACTATGTTTATGAACATACCCTGACAGGGAAAAATGCATTTTTTGTGGGAGATCTTGGAAA; GATTGTGAAGAAACACAGTCAATGGCAGAATGTAGTGGCTCAGATAAAGCCATTCTACACAGTGAAGTGC; AACTCTGCTCCAGCTGTACTTGAGATTTTGGCAGCTCTTGGAACCGGATTTGCTTGTTCCAGTAAAAATG; AAATGGCTTTAGTGCAAGAGTTGGGTGTACCTCCAGAAAACATTATTTACATAAGTCCTTGCAAGCAAGT; GTCTCAGATAAAGTATGCAGCAAAAGTTGGAGTGAATATCCTGACATGTGACAATGAAATTGAATTGAAG; AAAATTGCACGTAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG; AAGAGGGTAACATGAAGTTTGGCACTACCCTGAAGAACTGTAGGCATCTCTTGGAATGTGCTAAGGAACT; TGATGTCCAAATAATTGGGGTTAAATTTCATGTTTCGAGTGCTTGCAAAGAATCTCAAGTATATGTACAT; GCTCTATCTGATGCTCGATGTGTGTTTGACATGGCTGGAGAAATTGGCTTTACGATGAACATGTTAGACA; TTGGTGGAGGATTCACGGGAACTGAATTTCAATTGGAAGAGGTTAATCATGTTATCAGCCCTCTGTTGGA; TATCTACTTTCCTGAAGGATCTGGTGTTAAGATAATTTCAGAACCCGGAAGCTACTATGTGTCTTCTGCA; TTTACACTCGCAGTTAATATCATAGCAAAGAAAGTTGTTGAAAATGATAAATTTCCCTCTGGAGTAGAAA; AAACCGGAAGTGATGAACCAGCCTTCATGTATTATATGAATGATGGTGTTTATGGTTCTTTTGCAAGTAA; ACTGTCTGAGGACTTAAATACCATTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA; AGCAGCCTTTGGGGTCCATCCTGTGATGAGCTTGATCAAATTGTGGAAAGCTGTCTTCTTCCTGAGCTGA; ATGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTTCCATGAACCATCTGCTTTTAATGA; TTTTCAGAGGCCAGCCATTTATTACATGATGTCATTCAGTGATTGGTATGAGATGCAAGATGCTGGAATT; ACTTCAGACTCAATGATGAAGAACTTCTTCTTTGTGCCTTCTTGCATTCAGCTGAGCCAAGAAGACAGCT; TTTCCGCTGAAGCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200022 protein sequence; Red=Cloning site Green=Tags(s); ; MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQWQNVVAQIKPFYTVKC; NSAPAVLEILAALGTGFACSSKNEMALVQELGVPPENIIYISPCKQVSQIKYAAKVGVNILTCDNEIELK; KIARNHPNAKVLLHIATEDNIGGEEGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKESQVYVH; ALSDARCVFDMAGEIGFTMNMLDIGGGFTGTEFQLEEVNHVISPLLDIYFPEGSGVKIISEPGSYYVSSA; FTLAVNIIAKKVVENDKFPSGVEKTGSDEPAFMYYMNDGVYGSFASKLSEDLNTIPEVHKKYKEDEPLFT; SSLWGPSCDELDQIVESCLLPELNVGDWLIFDNMGADSFHEPSAFNDFQRPAIYYMMSFSDWYEMQDAGI; TSDSMMKNFFFVPSCIQLSQEDSFSAEA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015878
• ORF Size: 1344 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015878.5
• RefSeq Size: 4385 bp
• RefSeq ORF: 1347 bp
• Locus ID: 51582
• UniProt ID: O14977
• Cytogenetics: 8q22.3
• Domains: Orn_Arg_deC_N
• Protein Families: Druggable Genome
• MW: 49.5 kDa
• Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]