Cacna1c (NM_001256002) Mouse Tagged ORF Clone

SKU MR231934

Cacna1c (myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 10

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cacna1c
• Synonyms: Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231934 representing NM_001256002; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC; GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG; GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC; GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG; GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG; CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC; TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT; ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC; CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA; ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA; AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT; CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC; ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA; TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA; GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC; TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATG; ATGCCGTAGGAAGGGACTGGCCCTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACT; TAACTTGGTTCTCGGTGTTCTTAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT; TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG; CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCC; AGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGC; ATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACT; GTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATT; CTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTC; CTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAG; CCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGC; CTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTG; GTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCA; AGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGC; CTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGG; AAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTG; TGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCC; CTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTG; AATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGG; AGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGA; GAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCC; CCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCA; ACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCT; GTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCA; AACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCT; TCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATAT; CCTAGGCAATGCAGACTATGTCTTCACTAGTATCTTTACATTAGAAATTATCCTTAAGATGACTGCTTAC; GGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCG; TGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGT; CCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCC; ATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCC; AGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAA; CTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAG; TTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAG; AGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGAT; CTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTC; ATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAAT; GTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGT; GTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGC; CTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCA; CCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGCACTATTTCTGTGATGC; ATGGAATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAATCACCGAGGTACACCCA; GCTGAACATACCCAATGCTCTCCCTCTATGGTAAGAACAACCCTCCAGACCACATTTCCAGCTTCTGTGG; CTCCCCCTCTAGCCACCCACGTCTTATCCACCCTGATTTCCAGAGCCCTGGGAACCCAGCCCAGCTCCCA; TTGCATGTGGCCACCATGTCCGTTGTGTGCCTTAACTGCCTGTGCTCACGTGAGCTGTGCAAGGAGTGCA; GAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGA; GCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGC; TCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTG; AATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCA; GGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGA; GTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATC; AGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACT; ACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTA; TGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCA; CTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGA; ACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAAC; AGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGC; ATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCA; CCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTC; ACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGG; GCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCT; CCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAG; CAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAA; GCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCA; CGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTA; CTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCA; GCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATAT; TTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTC; CACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAG; ATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGG; AATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGT; TCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTC; CCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCC; TACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTG; GTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACC; GTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCT; TGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGC; TGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAG; CAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCC; CAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTC; CTACGTCAGCAACCTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231934 representing NM_001256002; Red=Cloning site Green=Tags(s); ; MVNENTRMYVPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGN; ATISTVSSTQRKRQQYGKPKKQGGTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVA; LAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSA; ILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVII; IYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFA; FAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD; FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNRGAPAGLHDQKKGKFAWFSHSTETHVS; MPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVF; LNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETIL; VETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGG; KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILL; NVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGES; PPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSP; NNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAY; GAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVA; IRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSK; FDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFV; IVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTIC; LAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVHP; AEHTQCSPSMVRTTLQTTFPASVAPPLATHVLSTLISRALGTQPSSHCMWPPCPLCALTACAHVSCARSA; EENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAL; NDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFI; SFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPP; LGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTS; MKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRR; AISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVTYYQSDSRGNFPQTFATQRPLHINKTGNNQ; ADTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYSSTVSTVEGHGPPLSPAVRVQEA; AWKLSSKRCHSRESQGATVNQEIFPDETRSVRMSEEAEYCSEPSLLSTDMFSYQEDEHRQLTCPEEDKRE; IQPSPKRSFLRSASLGRRASFHLECLKRQKDQGGDISQKTALPLHLVHHQALAVAGLSPLLQRSHSPTTF; PRPCPTPPVTPGSRGRPLRPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETTACSGSSSMARRARPVSLT; VPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMENAADNILSGGAQ; QSPNGTLLPFVNCRDPGQDRAVAPEDESCAYALGRGRSEEALADSRSYVSNL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256002
• ORF Size: 6666 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001256002.2, NP_001242931.1
• RefSeq Size: 13841 bp
• RefSeq ORF: 6669 bp
• Locus ID: 12288
• Cytogenetics: 6 55.86 cM
• MW: 249.3 kDa
• Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]