Tjp1 (NM_009386) Mouse Tagged ORF Clone

SKU MR227561

Tjp1 (Myc-DDK-tagged) - Mouse tight junction protein 1 (Tjp1), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Tjp1
• Synonyms: ZO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227561 representing NM_009386; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG; TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT; TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG; GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC; AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG; TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA; GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG; AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT; CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA; ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG; GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA; AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT; GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC; ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC; GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG; GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA; AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT; GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC; AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG; GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT; TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC; CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG; ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT; TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT; CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG; CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT; TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC; CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG; CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG; AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG; GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA; TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC; TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA; ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC; AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA; CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT; TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG; ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG; AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA; GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCCTCATCTCCAGTCCCTTACCTTTCGC; CTGAAACAACCCCAGCATCATCAGCCTCTGCAGTTAATCATAATGTCAGTGTAACTAATGTCAGCCTGGA; GGAGCCTGCCCCAGCCCCTCCCACCTCGCACGCATCACAGCCTGGTTGTTTAGGAGCACCAAGTGCTGAG; GCAGCTCACGTAGTGCTCAGAGGTGAAGGACCACCATTGCCGCCGCATGCAGACCCAGCAAAGGTGTACA; GGAAGGAGCCGTATTCTGAAGAAATGATGAGACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCC; AGGGCAGAGGCCAGATAAAGAGCCAAATCTAGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCC; AGCAGAGACCTTGAGCAGCCGTCATACAGGTATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACT; ATGACCATCGCCTACGGTTTGAAGATCGAATCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAA; ACAGCCCTACCAACCTCGGCCTTTTGAGAATCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAA; GAGGCTTCAGAACGAGGTTATTTCCAGCGTTTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACAC; GCTATGAGCAGCTGCCTCGAACCTCTACTCTACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCA; CAACAGGTACAGGCCAGAGGCACAGCCCTATTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTAC; TTTGACCAGTACCCGCGAAGTTATGAGCAAGTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACG; AGCCTCTCCATGGTGCTGCAGTTGTCCCTCCTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCC; CTCGGCTACCAAACCACAGCCTCCACCCCCAACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCA; CAGTCTGTGCTCACCAGAGTCAAAATGTTTGAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATG; TGAATGACACTGCCAGCTTTAAGCCTCCGGAAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCC; TAAACCTGTCCCTCAGAGTCAGTTTAGTGAGCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAA; CCTCAAGTGAAGCCACCCGAAGATATTGTTCGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATT; ACCGGAAACAGCTCTCCTACTTTGACCGAAGAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGG; CCATCACTCAGAGCCTGCTAAGCCAGTCCATTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAG; GGAAAACCCGAAACTGATGCTGTGGATAGATCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGC; CTCCTCCTCCTCCGTTGCCCTCACAGTACAGCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACAT; ACATTCCAAGGGCGCCCAGGGTGAAGGCAACTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAA; CCAGACCCACCCCCATCTCAGAGTAAACCAGCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGA; CTTTCTATCCGCAGAAAAGTTTCCCAGACAAAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCAT; CACTCCGGTGTACAATCGATTCACACCAAAGCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTT; GAAAGTCCGAAGTTCAACCATAATCTTCTGCCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAA; CTCCCACTTCCCCAAAAACTCTTATGAAAGCTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGT; TGAAACTTTCTCTGTTCACACAGATAAGCCTAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCT; GTCCCTGTGAGTCCTTCAGCTGTGGAAGAAGATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTC; GTGGCATTTTTAACAGCAATGGTGGTGTGTTGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACA; AGGAGCCATTCCTGAAGGAATTGAGCAAGAAATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCA; CCTTTAGATAAAGAGAAAGGTGAAACTCTGCTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGT; TCCTGAAGCCCGTGGAGCTACGCTTGCCACACTGTGACCCTAAAACCTGGCAAAACAAGTGTCTTCCTGG; AGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTCCTGATTGACCACTTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227561 representing NM_009386; Red=Cloning site Green=Tags(s); ; MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQ; ENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPR; AGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKE; ISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHS; ANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPG; AISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRP; SMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDL; PKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAI; RIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKF; PAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQ; DKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFT; TTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHT; SDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRI; DSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAE; AAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQA; SRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPE; EASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQY; FDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKP; QSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQK; PQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSK; GKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSK; PDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKF; ESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKA; VPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILP; PLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009386
• ORF Size: 5235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009386.2, NP_033412.2
• RefSeq Size: 7054 bp
• RefSeq ORF: 5238 bp
• Locus ID: 21872
• UniProt ID: P39447
• Cytogenetics: 7 35.02 cM
• MW: 195.2 kDa
• Summary: Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]