Fkbp4 (NM_010219) Mouse Tagged ORF Clone

SKU MR227193

Fkbp4 (Myc-DDK-tagged) - Mouse FK506 binding protein 4 (Fkbp4)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Fkbp4
• Synonyms: 59kDa; AL022792; AW208983; FKBP-4; FKBP-52; FKBP52; FKPB52; p59
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227193 representing NM_010219; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCGCCGAGGAGATGAAGGCGGCGGAGAACGGGGCGCAGTCGGCGCCCCTGCCTCTCGAAGGAGTGG; ACATCAGCCCCAAACAGGACGAGGGCGTGCTCAAGGTCATCAAGAGAGAGGGTACAGGCACAGAGACACC; CATGATCGGGGACCGAGTCTTTGTCCACTACACTGGCTGGCTGCTAGATGGCACAAAGTTTGACTCCAGT; CTGGACCGCAAGGACAAATTCTCCTTTGACCTGGGAAAAGGGGAGGTCATCAAGGCTTGGGATATTGCTG; TGGCAACCATGAAAGTGGGGGAAGTGTGCCACATCACCTGCAAGCCAGAATATGCCTATGGCGCAGCAGG; CAGCCCTCCGAAGATCCCCCCCAACGCCACACTTGTATTTGAGGTGGAGCTGTTTGAGTTCAAAGGAGAA; GATCTTACAGAAGAAGAAGATGGCGGGATCATCCGCAGAATACGGACTCGGGGTGAAGGCTATGCCAGGC; CCAATGATGGTGCTATGGTGGAAGTGGCCCTGGAAGGCTACCACAAGGACCGCCTCTTTGACCAGCGGGA; GCTCTGCTTTGAAGTCGGGGAAGGGGAAAGTCTAGATCTGCCCTGTGGGCTGGAGGAGGCCATTCAGCGC; ATGGAGAAAGGAGAGCATTCCATCGTGTACCTCAAACCTAGCTATGCTTTTGGCAGTGTGGGGAAGGAGA; GGTTCCAGATCCCACCGCACGCTGAGCTGAGGTATGAAGTGCGGCTGAAGAGCTTTGAGAAGGCCAAGGA; GTCTTGGGAGATGAGCTCCGCGGAGAAGCTGGAGCAGAGCAACATAGTGAAAGAGAGGGGCACCGCGTAC; TTCAAGGAAGGCAAGTACAAGCAGGCGTTACTGCAGTACAAGAAGATCGTGTCTTGGCTAGAATACGAGT; CTAGCTTCTCCGGTGAGGAAATGCAAAAGGTCCATGCACTCCGACTGGCCTCACACCTCAATCTGGCCAT; GTGTCACCTGAAACTGCAGGCCTTCTCAGCTGCCATCGAAAGCTGCAACAAGGCCTTGGAGCTGGACAGC; AACAACGAGAAGGGCCTGTTTCGCCGGGGAGAGGCCCACCTGGCCGTGAATGACTTTGACCTGGCAAGAG; CTGACTTCCAAAAGGTCCTGCAGCTCTATCCCAGCAACAAAGCCGCCAAGACCCAGCTGGCTGTGTGCCA; GCAGCGGACCCGTAGGCAGCTCGCCCGGGAAAAGAAGCTCTATGCCAACATGTTTGAGAGGCTGGCTGAG; GAGGAGCACAAGGTGAAGGCAGAAGTGGCAGCAGGAGACCATCCCACTGATGCTGAGATGAAGGGTGAGC; GGAACAATGTGGCCGAGAACCAGTCTCGGGTGGAGACAGAAGCG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227193 representing NM_010219; Red=Cloning site Green=Tags(s); ; MTAEEMKAAENGAQSAPLPLEGVDISPKQDEGVLKVIKREGTGTETPMIGDRVFVHYTGWLLDGTKFDSS; LDRKDKFSFDLGKGEVIKAWDIAVATMKVGEVCHITCKPEYAYGAAGSPPKIPPNATLVFEVELFEFKGE; DLTEEEDGGIIRRIRTRGEGYARPNDGAMVEVALEGYHKDRLFDQRELCFEVGEGESLDLPCGLEEAIQR; MEKGEHSIVYLKPSYAFGSVGKERFQIPPHAELRYEVRLKSFEKAKESWEMSSAEKLEQSNIVKERGTAY; FKEGKYKQALLQYKKIVSWLEYESSFSGEEMQKVHALRLASHLNLAMCHLKLQAFSAAIESCNKALELDS; NNEKGLFRRGEAHLAVNDFDLARADFQKVLQLYPSNKAAKTQLAVCQQRTRRQLAREKKLYANMFERLAE; EEHKVKAEVAAGDHPTDAEMKGERNNVAENQSRVETEA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010219
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_010219.4
• RefSeq Size: 2167 bp
• RefSeq ORF: 1377 bp
• Locus ID: 14228
• UniProt ID: P30416
• Cytogenetics: 6 F3
• MW: 52 kDa
• Summary: Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.[UniProtKB/Swiss-Prot Function]