Sox9 (NM_011448) Mouse Tagged ORF Clone

CAT#: MR227031

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  • TrueORF®

Sox9 (Myc-DDK-tagged) - Mouse SRY-box containing gene 9 (Sox9)



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Reconstitution Protocol

USD 708.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Sox9
Synonyms 2010306G03Rik; AV220920; mKIAA4243
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR227031 representing NM_011448
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCTCCTGGACCCCTTCATGAAGATGACCGACGAGCAGGAGAAGGGCCTGTCTGGCGCCCCCAGCC
CCACCATGTCGGAGGACTCGGCTGGTTCGCCCTGTCCCTCGGGCTCCGGCTCGGACACGGAGAACACCCG
GCCCCAGGAGAACACCTTCCCCAAGGGCGAGCCGGATCTGAAGAAGGAGAGCGAGGAAGATAAGTTCCCC
GTGTGCATCCGCGAGGCGGTCAGCCAGGTGCTGAAGGGCTACGACTGGACGCTGGTGCCCATGCCCGTGC
GCGTCAACGGCTCCAGCAAGAACAAGCCACACGTCAAGCGACCCATGAACGCCTTCATGGTGTGGGCGCA
GGCTGCGCGCAGGAAGCTGGCAGACCAGTACCCGCATCTGCACAACGCGGAGCTCAGCAAGACTCTGGGC
AAGCTCTGGAGGCTGCTGAACGAGAGCGAGAAGAGACCCTTCGTGGAGGAGGCGGAGCGGCTGCGCGTGC
AGCACAAGAAAGACCACCCCGATTACAAGTACCAGCCCCGGCGGAGGAAGTCGGTGAAGAACGGACAAGC
GGAGGCCGAAGAGGCCACGGAACAGACTCACATCTCTCCTAATGCTATCTTCAAGGCGCTGCAAGCCGAC
TCCCCACATTCCTCCTCCGGCATGAGTGAGGTGCACTCCCCGGGCGAGCACTCTGGGCAATCTCAGGGTC
CGCCGACCCCACCCACCACTCCCAAAACCGACGTGCAAGCTGGCAAAGTTGATCTGAAGCGAGAGGGGCG
CCCTCTGGCAGAGGGGGGCAGACAGCCCCCCATCGACTTCCGCGACGTGGACATCGGTGAACTGAGCAGC
GACGTCATCTCCAACATTGAGACCTTCGACGTCAATGAGTTTGACCAATACTTGCCACCCAACGGCCACC
CAGGGGTTCCGGCCACCCACGGCCAGGTCACCTACACTGGCAGTTACGGCATCAGCAGCACCGCACCCAC
CCCTGCGACCGCGGGCCACGTGTGGATGTCGAAGCAGCAGGCGCCGCCCCCTCCTCCGCAGCAGCCTCCG
CAGGCCCCGCAAGCCCCACAGGCGCCTCCGCAGCAGCAAGCACCCCCGCAGCAGCCGCAGGCACCCCAGC
AGCAGCAGGCACACACGCTCACCACGCTGAGCAGCGAGCCAGGCCAGTCCCAGCGAACGCACATCAAGAC
GGAGCAGCTGAGCCCCAGCCACTACAGCGAGCAGCAGCAGCACTCCCCGCAACAGATCTCCTACAGCCCC
TTCAACCTTCCTCACTACAGCCCCTCCTACCCGCCCATCACCCGCTCGCAATACGACTACGCTGACCATC
AGAACTCCGGCTCCTACTACAGTCACGCAGCCGGCCAGGGCTCAGGGCTCTACTCCACCTTCACTTACAT
GAACCCCGCGCAGCGCCCCATGTACACCCCCATCGCTGACACCTCCGGGGTCCCTTCCATCCCGCAGACC
CACAGCCCGCAGCACTGGGAACAACCAGTCTACACACAGCTCACCAGACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227031 representing NM_011448
Red=Cloning site Green=Tags(s)

MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFP
VCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLG
KLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQAD
SPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQAGKVDLKREGRPLAEGGRQPPIDFRDVDIGELSS
DVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAPTPATAGHVWMSKQQAPPPPPQQPP
QAPQAPQAPPQQQAPPQQPQAPQQQQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQISYSP
FNLPHYSPSYPPITRSQYDYADHQNSGSYYSHAAGQGSGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQT
HSPQHWEQPVYTQLTRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011448
ORF Size 1521 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011448.4, NP_035578.3
RefSeq Size 4146 bp
RefSeq ORF 1524 bp
Locus ID 20682
UniProt ID Q04887
Cytogenetics 11 77.27 cM
MW 56.5 kDa
Gene Summary Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development (PubMed:20940257, PubMed:28263186). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (PubMed:20940257).[UniProtKB/Swiss-Prot Function]
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