Sox9 (NM_011448) Mouse Tagged ORF Clone

CAT#: MG227031

Sox9 (tGFP-tagged) - Mouse SRY-box containing gene 9 (Sox9), (10ug)

Product Images

Circular map for MG227031

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sox9
• Synonyms: 2010306G03Rik; AV220920; mKIAA4243
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227031 representing NM_011448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTCCTGGACCCCTTCATGAAGATGACCGACGAGCAGGAGAAGGGCCTGTCTGGCGCCCCCAGCC
  CCACCATGTCGGAGGACTCGGCTGGTTCGCCCTGTCCCTCGGGCTCCGGCTCGGACACGGAGAACACCCG
  GCCCCAGGAGAACACCTTCCCCAAGGGCGAGCCGGATCTGAAGAAGGAGAGCGAGGAAGATAAGTTCCCC
  GTGTGCATCCGCGAGGCGGTCAGCCAGGTGCTGAAGGGCTACGACTGGACGCTGGTGCCCATGCCCGTGC
  GCGTCAACGGCTCCAGCAAGAACAAGCCACACGTCAAGCGACCCATGAACGCCTTCATGGTGTGGGCGCA
  GGCTGCGCGCAGGAAGCTGGCAGACCAGTACCCGCATCTGCACAACGCGGAGCTCAGCAAGACTCTGGGC
  AAGCTCTGGAGGCTGCTGAACGAGAGCGAGAAGAGACCCTTCGTGGAGGAGGCGGAGCGGCTGCGCGTGC
  AGCACAAGAAAGACCACCCCGATTACAAGTACCAGCCCCGGCGGAGGAAGTCGGTGAAGAACGGACAAGC
  GGAGGCCGAAGAGGCCACGGAACAGACTCACATCTCTCCTAATGCTATCTTCAAGGCGCTGCAAGCCGAC
  TCCCCACATTCCTCCTCCGGCATGAGTGAGGTGCACTCCCCGGGCGAGCACTCTGGGCAATCTCAGGGTC
  CGCCGACCCCACCCACCACTCCCAAAACCGACGTGCAAGCTGGCAAAGTTGATCTGAAGCGAGAGGGGCG
  CCCTCTGGCAGAGGGGGGCAGACAGCCCCCCATCGACTTCCGCGACGTGGACATCGGTGAACTGAGCAGC
  GACGTCATCTCCAACATTGAGACCTTCGACGTCAATGAGTTTGACCAATACTTGCCACCCAACGGCCACC
  CAGGGGTTCCGGCCACCCACGGCCAGGTCACCTACACTGGCAGTTACGGCATCAGCAGCACCGCACCCAC
  CCCTGCGACCGCGGGCCACGTGTGGATGTCGAAGCAGCAGGCGCCGCCCCCTCCTCCGCAGCAGCCTCCG
  CAGGCCCCGCAAGCCCCACAGGCGCCTCCGCAGCAGCAAGCACCCCCGCAGCAGCCGCAGGCACCCCAGC
  AGCAGCAGGCACACACGCTCACCACGCTGAGCAGCGAGCCAGGCCAGTCCCAGCGAACGCACATCAAGAC
  GGAGCAGCTGAGCCCCAGCCACTACAGCGAGCAGCAGCAGCACTCCCCGCAACAGATCTCCTACAGCCCC
  TTCAACCTTCCTCACTACAGCCCCTCCTACCCGCCCATCACCCGCTCGCAATACGACTACGCTGACCATC
  AGAACTCCGGCTCCTACTACAGTCACGCAGCCGGCCAGGGCTCAGGGCTCTACTCCACCTTCACTTACAT
  GAACCCCGCGCAGCGCCCCATGTACACCCCCATCGCTGACACCTCCGGGGTCCCTTCCATCCCGCAGACC
  CACAGCCCGCAGCACTGGGAACAACCAGTCTACACACAGCTCACCAGACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227031 representing NM_011448
  Red=Cloning site Green=Tags(s)
  
  MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFP
  VCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLG
  KLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQAD
  SPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQAGKVDLKREGRPLAEGGRQPPIDFRDVDIGELSS
  DVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAPTPATAGHVWMSKQQAPPPPPQQPP
  QAPQAPQAPPQQQAPPQQPQAPQQQQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQISYSP
  FNLPHYSPSYPPITRSQYDYADHQNSGSYYSHAAGQGSGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQT
  HSPQHWEQPVYTQLTRP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011448
• ORF Size: 1521 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011448.4, NP_035578.3
• RefSeq Size: 4146 bp
• RefSeq ORF: 1524 bp
• Locus ID: 20682
• UniProt ID: Q04887
• Cytogenetics: 11 77.27 cM
• Gene Summary: Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development (PubMed:20940257, PubMed:28263186). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (PubMed:20940257).[UniProtKB/Swiss-Prot Function]