Rfx6 (NM_001159389) Mouse Tagged ORF Clone

SKU MR224903

Rfx6 (Myc-DDK-tagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Rfx6
• Synonyms: 4930572O07Rik; Rfxdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224903 representing NM_001159389; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTAAGGTCCGGGAACTGGAGGAAGCTTTCGTGCAGGAGCAGCCTTCGCCCCAACTTCCCTCCGAGA; TCGCGGAGGAGTGCTGCGCGCAGCTGCTTGGCAAGGGCTTGCTGGTCTACCCTGAGGACAGTGCTTACCT; GCTGGCTGAAACAGCCGCGGGCGCTCGGAGCAGCGGTGAGAAAGGCGGAGATCCCGGGCTGCAGGTGGGA; GTTAAATCAGAAATGCAGTTAAACAACGGCAACTTTTCCTCGGAAGAAGAGGATGCGGACACCCAGGAGA; GCAAGACCAAAGCAGCCGACCCACAGCTCTCTCAGAAGAAGAGCATCACGCAGATGATGAAGGATAAGAA; GAAGCAGACTCAGCTCACCCTGCAGTGGCTTGAAGACAATTACATAGTGTGTGAAGGAGTTTGCCTCCCA; CGGTGCATTCTTTATGCTCACTACCTAGATTTCTGCAGGAAAGAGAAACTGGAGCCAGCTTGTGCAGCCA; CCTTTGGAAAGACAATTCGCCAAAAATTTCCCCTCCTAACAACTCGAAGACTTGGAACAAGAGGACATTC; AAAGTACCATTACTATGGGATTGGCATCAAAGAAAGCAGTGCATATTACCACTCTGTTTATTCTGGAAAG; GGCTTGACAAGGTTTTCGGGGAGCAAACTGAAGAATGAGGGTGGTTTTACACGTAAATACTCACTTAGCT; CAAAAACAGGAACACTTCTTCCAGAATTTCCCAGCGCTCAACATCTTGTGTACCAAGGATGCATTTCTAA; GGACAAGGTTGATACTCTCATAATGATGTACAAAACTCACTGCCAGTGCATACTCGACAATGCCATCAAT; GGGAACTTTGAAGAGATCCAGCATTTCTTACTACACTTTTGGCAAGGAATGCCAGACCATCTCCTTCCCC; TGCTTGAAAATCCTGTTATCATTGATATTTTCTGTGTCTGTGACTCAATTCTTTATAAGGTTCTTACAGA; TGTCCTCATTCCTGCAACAATGCAAGAAATGCCTGAAAGTTTGTTAGCAGATATAAGAAATTTTGCTAAA; AATTGGGAACAGTGGGTTGTTTCATCCTTGGAAAACTTGCCAGAAGCCCTCATTGATAAGAAAATCCCCA; TTTTGCGAAGATTTGTATCTTCCCTGAAGCGACAGACATCTTTCTTGCATCTTGCTCAGATTGCCAGACC; AGCTCTCTTTGACCAGCATGTGGTGAATGCCATGGTATCTGATATTGAAAAGGTTGACTTAAATAGTATT; GGGTCTCAGGCTCTTCTTACCATATCCAACAGCACAGACACGGAATCTGACATCTACAGTGAACATGACT; CTATTACTGTGTTCCAAGAACTGAAAGATCTCCTTAAGAAGAATGCTACAGTGGAGGCATTTATTGAATG; GTTGGACACTGTGGTAGAGCAGAGAGTTATTAAGATGAGCAAACAAAATGGAAGATCTCTGAAGAAGAGG; GCTCAAGACTTTCTGCTCAAATGGAGCTTTTTTGGTGCCCGCGTGATGCATAATCTTACCTTGAACAACG; CATCAAGTTTTGGCTCTTTCCATCTGATCCGAATGCTTCTGGATGAGTACATTCTCCTGGCCATGGAGAC; TCAATTTAACAATGACAAAGAGCAGGAACTACAGAATTTATTGGACAAGTATATGAAGAACTCGGATGCG; AGTAAAGCTGCCTTCACAGCTTCCCCGAGCTCTTGCTTTCTGGCCAACCGAAATAAGGCTAGCTCACTTG; CCAGTGACACTGTGAAGAACGAAAGCCACGTGGAGACATCCTATGTCCCTCTGCCTTCCAGCCAGCCTGG; AGCCATACCCCCTGCTCTGCACCCATTCTCAACTGAGGACACTGATAACATGCCACTCCCAGGTCAAATA; GAGCTTTCACAAAGTACTGGCCATCTGATGACACCACCGATTTCTCCAGCCATAGCAAGCAGAGGAAGTG; TTATTAACCAAGGGCCAATGGCGAGCAGACCCCCGAGCGTGGGCACAGTTCTCTCAGCTCCAACACATTG; CTCAACATATGCAGAACCAATTTATCCTACGCTCTCTCCAGCCAACCACGACTTTTATGGGACCAACTCT; AACTATCAGACTATGTTTAGGACACAGTCTCACCCTGCATCAAGCCTCTATGCTCACCGTGCAGAGCATG; GGCGGTGCATGGCCTGGACTGAACAGCAGCTTTCTAGAGACTTCTTTGGTGGCAGTTGTGCTGGGTCTCC; ATATAATTGTAGGCCACCTTCCAGTTATGGACCATCCACACACACACAAGAGTCACACAGCATGCAGGTT; TTGAACACAGGAAGCTTCAATTTCCTTAGTAATGCAGGAGCTGGCAGCTGCCAAGGGTCAACATTGCCTT; CTAATTCTCCCAATGGATACTATGGAAACAATATAAACTACTCAGAGGCACATAGGCTTGGATCGATGGT; GAACCAACATGTTTCAGTCATCAGCAGTGTGCGCTCCCTGCCTCCCTACAGTGATATTCATGATCCACTT; AACATTTTAGATGACAGCAGCCGGAAGCAGAACAACTCGTTTTATGCAGACACATTGTCTCCTGTTGCAT; GTCGTACTACTGTAGTAGCTTCCAACTTGCAAACCCAGATTCCTTCATCTTCATCCCAGTGTATGTATGG; AACTTCCAATCAGTATCCAGTGCAAGATAGTCTGGACTCCAATGCAGCAAGCAACAGAGAAATGGTGTCC; TCTTTACCACCCATCAACACCGTGTTTATGGGGACAGCAGCTGGAGACACT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224903 representing NM_001159389; Red=Cloning site Green=Tags(s); ; MAKVRELEEAFVQEQPSPQLPSEIAEECCAQLLGKGLLVYPEDSAYLLAETAAGARSSGEKGGDPGLQVG; VKSEMQLNNGNFSSEEEDADTQESKTKAADPQLSQKKSITQMMKDKKKQTQLTLQWLEDNYIVCEGVCLP; RCILYAHYLDFCRKEKLEPACAATFGKTIRQKFPLLTTRRLGTRGHSKYHYYGIGIKESSAYYHSVYSGK; GLTRFSGSKLKNEGGFTRKYSLSSKTGTLLPEFPSAQHLVYQGCISKDKVDTLIMMYKTHCQCILDNAIN; GNFEEIQHFLLHFWQGMPDHLLPLLENPVIIDIFCVCDSILYKVLTDVLIPATMQEMPESLLADIRNFAK; NWEQWVVSSLENLPEALIDKKIPILRRFVSSLKRQTSFLHLAQIARPALFDQHVVNAMVSDIEKVDLNSI; GSQALLTISNSTDTESDIYSEHDSITVFQELKDLLKKNATVEAFIEWLDTVVEQRVIKMSKQNGRSLKKR; AQDFLLKWSFFGARVMHNLTLNNASSFGSFHLIRMLLDEYILLAMETQFNNDKEQELQNLLDKYMKNSDA; SKAAFTASPSSCFLANRNKASSLASDTVKNESHVETSYVPLPSSQPGAIPPALHPFSTEDTDNMPLPGQI; ELSQSTGHLMTPPISPAIASRGSVINQGPMASRPPSVGTVLSAPTHCSTYAEPIYPTLSPANHDFYGTNS; NYQTMFRTQSHPASSLYAHRAEHGRCMAWTEQQLSRDFFGGSCAGSPYNCRPPSSYGPSTHTQESHSMQV; LNTGSFNFLSNAGAGSCQGSTLPSNSPNGYYGNNINYSEAHRLGSMVNQHVSVISSVRSLPPYSDIHDPL; NILDDSSRKQNNSFYADTLSPVACRTTVVASNLQTQIPSSSSQCMYGTSNQYPVQDSLDSNAASNREMVS; SLPPINTVFMGTAAGDT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159389
• ORF Size: 2781 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001159389.1, NP_001152861.1
• RefSeq Size: 3457 bp
• RefSeq ORF: 2784 bp
• Locus ID: 320995
• UniProt ID: Q8C7R7
• Cytogenetics: 10 B3
• MW: 102.6 kDa
• Summary: Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]