Ppp1r9b (NM_172261) Mouse Tagged ORF Clone

SKU MR222264

Ppp1r9b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory subunit 9B (Ppp1r9b)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ppp1r9b
• Synonyms: SPL; Spn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222264 representing NM_172261; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGAAGACGGAGCCTCGGGGGCCCGGGGGTCCCCTCCGGAGCGCCTCCCCGCACCGCAGCGCCTACG; AAGCGGGCATCCAGGCACTAAAGCCTCCCGATGCACCCGGGCCCGACGAGGCACCCAAGGCGGCCCACCA; TAAGAAATATGGCTCCAACGTCCACCGCATCAAAAGTATGTTCCTGCAGATGGGCACCACCGCGGGGCCG; CCGGGTGAGGCAGGCGGAGGCGCAGGCATGGCCGAGGCCCCACGGGCGTCCGATCGCGGGGTGCGCCTGT; CCCTGCCGCGGGCCAGCAGCCTGAACGAGAACGTAGACCACAGTGCCCTGCTCAAGCTGGGCACCAGTGT; GTCGGAGCGCGTGAGCCGCTTCGACTCCAAGCCCGCGCCCTCGGCGCAGCCCGCTCCTCCGCCGCACCCG; CCGTCCCGGCTGCAGGAGACGCGGAAGCTGTTCGAACGGAGCGTCCCCGCGGCCTCGGGTGGCGACAAGG; AGGCCGTGGCGCGGCGGCTGCTGAGGCAGGAGCGCGCCGGCCTGCAGGACCGGAAGCTGGACGTCGTGGT; GCGCTTTAACGGCAGCACCGAGGCGCTGGACAAGCTGGATGCTGACGCCGTGTCGCCGACGGTCAGCCAG; CTCAGTGCGGTCTTCGAGAAAGCCGACTCGAGGACCGGCCTTCACCGTGCACCCGGCCCCCCTCGGGCAG; CCGGGGCGCCCCAGGTCAACTCGAAGCTGGTCACTAAGCGGTCCCGGGTGTTCCAGCCACCGCCGCCGCC; TCCCGCCCCGTCGGGGGATGGCGCGACCGAGAAGGAGCGTGGCCCCGGAGGGCAGCAGCCCCCACAGCAC; CGGGTGGCCCCTGCCCGACCTCCGCCTAAGCCGCGGGAGGTGCGCAAGATCAAGCCGGTGGAGGTTGAAG; AAAGTGGGGAGTCTGAGGCCGAGTCGGCTCCCGGGGAAGTGATCCAGGCCGAGGTGACGGTCCATGCAGC; CTTGGAGAATGGCAGCACCCCGGCCACCACAGCCAGCCCTGCACCCGAGGAACCCAAGGCCGAAGCGGTC; CCCGAGGAGGAGGCGGCGGCGTCAGTGGCGACGCTAGAGAGGGGGGTGGACAATGGCCGGGCCCCGGACA; TGGCACCTGAGGAGGTGGATGAATCCAAGAAGGAGGACTTCTCGGAAGCGGACTTGGTGGATGTGAGCGC; CTACAGTGGACTCGGGGAGGACTCTGGGGGCAGTGCCCTGGAGGAGGACGATGAAGAAGACGAAGAGGAC; GGGGAGCCCCCCTATGAGCCGGAGTCAGGGTGTGTGGAGATTCCCGGGCTCTCAGAGGAAGAGGACCCGG; CCCCGAGCCGGAAGATCCATTTCAGCACCGCACCGATCCAAGTATTCAGCACCTACTCCAACGAGGACTA; TGACCGACGCAATGAGGATGTGGATCCCATGGCAGCCTCTGCGGAATATGAGCTAGAGAAGCGAGTGGAG; AGGTTGGAGCTGTTTCCTGTGGAGCTGGAGAAGGACTCTGAGGGCTTGGGCATCAGCATCATTGGCATGG; GCGCCGGAGCCGACATGGGCCTGGAGAAGCTGGGCATCTTCGTCAAGACTGTGACTGAGGGTGGTGCAGC; CCATCGGGATGGCAGGATCCAAGTGAACGACCTGCTGGTGGAGGTGGATGGGACGAGCCTGGTGGGAGTG; ACCCAGAGCTTTGCAGCCTCTGTACTTAGGAACACCAAAGGCCGAGTGCGGTTCATGATTGGCCGGGAGC; GGCCTGGAGAACAGAGTGAAGTGGCCCAGCTAATTCAGCAGACCTTGGAGCAGGAGCGATGGCAGCGGGA; GATGATGGAGCAGAGATACGCCCAGTATGGAGAGGATGATGAGGAGACAGGAGAGTATGCCACCGACGAA; GATGAAGAACTGAGCCCAACGTTCCCAGGGGGTGAAATGGCCATTGAGGTGTTTGAACTAGCAGAGAACG; AGGATGCGCTGTCCCCCGTGGAGATGGAGCCCGAAAAGCTGGTGCACAAGTTCAAGGAGCTCCAGATCAA; GCATGCTGTCACTGAAGCGGAAATCCAGCAGCTGAAACGGAAGTTGCAGAGTCTGGAGCAGGAGAAAGGG; CGCTGGCGGGTAGAGAAGGCCCAGCTGGAGCAGAGCGTGGAGGAGAATAAGGAGCGGATGGAGAAGCTAG; AGGGCTACTGGGGTGAGGCCCAGAGCCTGTGCCAGGCTGTGGATGAGCACCTGCGGGAGACCCAGGCCCA; GTATCAGGCCCTGGAGCGCAAGTACAGCAAGGCCAAGCGTCTCATCAAGGACTACCAGCAAAAGGAGATC; GAGTTCCTGAAAAAAGAGACAGCCCAGCGTCGGGTACTGGAAGAGTCGGAGCTAGCCAGGAAGGAGGAAA; TGGACAAACTTCTGGACAAGATCTCAGAACTGGAAGGAAACCTGCAGACACTGAGGAACTCCAATTCTAC; T; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222264 representing NM_172261; Red=Cloning site Green=Tags(s); ; MMKTEPRGPGGPLRSASPHRSAYEAGIQALKPPDAPGPDEAPKAAHHKKYGSNVHRIKSMFLQMGTTAGP; PGEAGGGAGMAEAPRASDRGVRLSLPRASSLNENVDHSALLKLGTSVSERVSRFDSKPAPSAQPAPPPHP; PSRLQETRKLFERSVPAASGGDKEAVARRLLRQERAGLQDRKLDVVVRFNGSTEALDKLDADAVSPTVSQ; LSAVFEKADSRTGLHRAPGPPRAAGAPQVNSKLVTKRSRVFQPPPPPPAPSGDGATEKERGPGGQQPPQH; RVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGSTPATTASPAPEEPKAEAV; PEEEAAASVATLERGVDNGRAPDMAPEEVDESKKEDFSEADLVDVSAYSGLGEDSGGSALEEDDEEDEED; GEPPYEPESGCVEIPGLSEEEDPAPSRKIHFSTAPIQVFSTYSNEDYDRRNEDVDPMAASAEYELEKRVE; RLELFPVELEKDSEGLGISIIGMGAGADMGLEKLGIFVKTVTEGGAAHRDGRIQVNDLLVEVDGTSLVGV; TQSFAASVLRNTKGRVRFMIGRERPGEQSEVAQLIQQTLEQERWQREMMEQRYAQYGEDDEETGEYATDE; DEELSPTFPGGEMAIEVFELAENEDALSPVEMEPEKLVHKFKELQIKHAVTEAEIQQLKRKLQSLEQEKG; RWRVEKAQLEQSVEENKERMEKLEGYWGEAQSLCQAVDEHLRETQAQYQALERKYSKAKRLIKDYQQKEI; EFLKKETAQRRVLEESELARKEEMDKLLDKISELEGNLQTLRNSNST; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172261
• ORF Size: 2451 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_172261.3, NP_758465.2
• RefSeq Size: 4345 bp
• RefSeq ORF: 2454 bp
• Locus ID: 217124
• UniProt ID: Q6R891
• Cytogenetics: 11 D
• MW: 90 kDa
• Summary: Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration (By similarity).[UniProtKB/Swiss-Prot Function]