Asphd1 (NM_001039645) Mouse Tagged Lenti ORF Clone

SKU
MR221118L3
Lenti ORF clone of Asphd1 (Myc-DDK-tagged) - Mouse aspartate beta-hydroxylase domain containing 1 (Asphd1)
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$450.00
3 Weeks*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asphd1
Synonyms A830007L07Rik; Gm168
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR221118).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001039645
ORF Size 327 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039645.1
RefSeq Size 518 bp
RefSeq ORF 330 bp
Locus ID 233879
UniProt ID Q2TA57
Cytogenetics 7 F3
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SKU Description Size Price
MC212974 Asphd1 (untagged) - Mouse aspartate beta-hydroxylase domain containing 1 (Asphd1), (10ug) 10 ug
$165.00
MG221118 Asphd1 (tGFP-tagged) - Mouse aspartate beta-hydroxylase domain containing 1 (Asphd1), (10ug) 10 ug
$350.00
MR221118 Asphd1 (Myc-DDK-tagged) - Mouse aspartate beta-hydroxylase domain containing 1 (Asphd1) 10 ug
$289.00
MR221118L4 Lenti ORF clone of Asphd1 (mGFP-tagged) - Mouse aspartate beta-hydroxylase domain containing 1 (Asphd1) 10 ug
$450.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.