Clasp2 (NM_029633) Mouse Tagged ORF Clone

SKU MR217994

Clasp2 (Myc-DDK-tagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Clasp2
• Synonyms: 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217994 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGCGGCTGATTTGCAAACGGATCTGTGATTATAAAAGCTTTGATGATGAGGAATCAGTGGATGGAA; ATAGGCCGTCGTCAGCTGCTTCAGCCTTCAAGGTTCCTGCACCTAAAACACCTGGGAATCCTGTCAGCAG; TGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGAGGTCCTTCTAAAGAAGGAGGGGCTGGAGCA; GTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTCCTTCTGTTCAGATCTATTCTAGTCGAGAAC; TTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGATGACAAACATGACTGGGACCAGCGTGCCAA; TGCGCTTAAGAAAATCAGATCACTGCTTGTTGCTGGAGCCGCACAGTATGATTGCTTTTTCCAGCACCTA; CGTTTGTTGGATGGAGCGCTTAAGCTGTCAGCTAAGGACCTCAGATCCCAGGTGGTCAGGGAAGCTTGCA; TCACTGTTGCTCACCTTTCAACAGTCTTGGGAAACAAATTTGATCACGGCGCTGAAGCCATCGTCCCTAC; GCTTTTTAATCTTGTCCCCAATAGTGCCAAAGTCATGGCGACCTCTGGATGTGCAGCAATCAGATTTATT; ATTCGGCATACCCATGTACCTAGACTTATTCCTTTAATAACAAGCAACTGCACATCAAAGTCAGTTCCTG; TAAGGAGACGTTCATTTGAATTTTTAGATTTGCTGCTGCAAGAATGGCAGACTCATTCACTGGAAAGACA; TGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGCATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCA; AGGAAGACATACATGGGCCTTAGGAACCACTTTCCTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGC; CATCATATCAGAAAAGTCTTCAAACTTACTTAAAGAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGA; CAGGTCCTCATCCAGCTCACAAGAAAGTCTCAATCGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCT; TCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCAGCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTT; CTCGAAGTGACATTGATGTGAATGCGGCAGCTGGTGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCG; AAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCAGGCTCCTATGCATCACTAGAGGATACTTCTGACAAG; ATGGATGGAACAGCATCTGATGATGGTCGGGTGAGAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAA; ATGCCAAGACCGACTCTAGAGGGAGAAGCCGGACAAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATC; TGGGTCTCCAGGAAGAGTTCTAACCACAACAGCCCTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTG; GTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGATCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCC; CATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTATTCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGA; AGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCGGTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGG; ATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCAGTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGG; AGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGACATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTA; TGAATCATATGGAATGCACTCAGATGATGATGCCAACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCC; TATAGCTCTCGAAATGGTAGTATTCCTACCTACATGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACA; GATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGAAGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCA; GAGAACGCTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCAGATCCTCAT; GGCAAGGTGTTCAGCATGTTCTTGGAGACTCTAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAG; ATTGGTTGTTTGTTCTGCTGACACAGCTGCTGAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGC; AAAAGTTCAGAAAGCCCTTGATATTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATG; AGATTTACAGTTGACCAGACCCAAACGCCAAGCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAA; CTCTGGCAAAGCAGATGGACCCAAGAGATTTTACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGT; CATCACTTGGACGACAGAGCCCAAAAGCTCTGATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTA; TTTGAACTCAATACCCCAGAGTTTACAATGTTACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTA; CTAAACTTCTTCACAATCACCTCCGGAACACTGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGAC; GAGACCAACACCTCGGTCACCAGCCAACTGGTCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAAT; ACGTTATCTCCAAGTGCATTTGATTACGATACAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTA; GAGGCGTCACTGAGGCAATCCAGAATTTCAGCTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAG; GGACCCTAAAAAGGAGGATGGTGACACAATATGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGT; GATGCTGCTGACGGCAGCCAGCCAGCTCTGGATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACT; CCTCTCCACGCTCCCGTGACTATAACCCATATAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGC; CCTCAAGGAAGCCATGTTTGATGATGACGCCGACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGAC; CTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGT; ATGAACTCATGAAGCTAACCCAGGAAGAATCTTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATT; GTTATTGCTTGAGACCCTTGGGGATAAAGAGCCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAA; ATCTTAAGGCATCAACCAGCAAGATTCAAAAACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCAC; ATAAAGATCCTCACAAAGAGGTGGTGAGATCTGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAG; TCCAGAGCAGTGCATCAAAGTGCTTTGTCCCATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCA; ATCAAAATGCAAACAAAAGTGATAGAGAGAGTATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCA; TGCCGGGTCTAATACAGGGTTATGATAATTCAGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGT; GGCTGTCCATGCAGTGATTGGTGATGAACTAAAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAG; CTGCTGAACCTTTACATCAAGCGTGCACAGACGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTG; GACAGAGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217994 protein sequence; Red=Cloning site Green=Tags(s); ; MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTPGNPVSSARKPGSAGGPKVGGPSKEGGAGA; VDEDDFIKAFTDVPSVQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHL; RLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFI; IRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEA; RKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANP; STVAGRVSVGGSKANPLPGSLQRSRSDIDVNAAAGAKAHHAAGQAVRSGRLGAGALNPGSYASLEDTSDK; MDGTASDDGRVRAKLSTPLVAVGNAKTDSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGAQRVL; VNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLGPGYG; ISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGMHSDDDANSDASSACSERS; YSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPH; GKVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITRESFPNDLQFNILM; RFTVDQTQTPSLKVKVAILKYIETLAKQMDPRDFTNSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISL; FELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQN; TLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMSEPVRRDPKKEDGDTICSGPGMSDPRAGG; DAADGSQPALDNKASLLHSMPLHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSD; LVAELLKELSNHNERIEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLKE; ILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAA; IKMQTKVIERVSKETLNMLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMK; LLNLYIKRAQTGSAGADPTADVSGQS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029633
• ORF Size: 3858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_029633.2, NP_083909.2
• RefSeq Size: 5624 bp
• RefSeq ORF: 3861 bp
• Locus ID: 76499
• Cytogenetics: 9 F3
• MW: 140.7 kDa
• Summary: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]