Slc24a2 (NM_172426) Mouse Tagged ORF Clone

SKU
MR215153
Slc24a2 (Myc-DDK-tagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 2 (Slc24a2), transcript variant 1
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$289.00 MSRP $620.00 MSRP $620.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc24a2
Synonyms 2810021B17Rik; 6330417K15Rik; AI847460; Nckx2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215153 representing NM_172426
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTCCACCAAAGCCCCACAGCCAGGCTCCTGCAGAAATGGTGTTCCCATGAGTCACCATTTGGCT
GCAGGAGACATTATAATAGCAGGAAAAAACTGAAGCTAATTCGAGTCATTGGCCTTGTCATGGGCCTGGT
AGCTGTGAGCACTGTCCCATTTTCAATCAGTGCCTTTACTGAGACAGACTCTCAGAGCAACAGAGGAGAA
GCCAGTGATATGAGTGGCCCCAGAGTAGCACAGGGTCACCGTCAAAGAACTCTCTTAGACTTAAATGACA
AGATTCGAGATTACACCCCACAGCCACCTGCTTCTCAGGAAGACCAAGCTGAGAACAGTACTGAGCACAC
CCAGGGAGACTACCCAAAGGACATCTTTTCCCTGGAGGAGCGAAGGAAAGGTGCCATCATTCTGCATGTC
ATTGGGATGATCTATATGTTCATAGCATTAGCCATCGTCTGTGATGAGTTCTTTGTTCCTTCTCTAACTG
TCATCACTGAAAAACTGGGAATCTCTGATGATGTGGCTGGAGCCACCTTCATGGCTGCAGGCGGGTCAGC
CCCAGAACTTTTCACGTCTCTTATAGGGGTCTTCATTGCTCACAGCAATGTCGGCATTGGCACCATCGTG
GGTTCCGCCGTGTTCAATATTCTCTTTGTTATTGGCATGTGTGCTCTGTTTTCTAGAGAAATCCTAAACC
TGACCTGGTGGCCGCTCTTTCGGGATGTGTCCTTCTACATCGTTGATTTGCTCATGTTGATCACATTTTT
CCTGGATAACGTTATCATGTGGTGGGAAAGCTTACTCCTCTTAACAGCTTATTTCGCCTATGTGGTTTTC
ATGAAGTTCAACGTCCAAGTAGAGAGATGGGTGAAGCAAATGATATCTCGCAATAATGTTATCAAGGTGA
CAGTGCCAGAAGCCCAAGCAAAGTCACCTACAGCTGGGGACAAGGATGGGCCAACTTTACCGTCGAAGCC
ACGCCTCCAACGAGGCGGAAGCTCTGCCTCCCTCCACAACAGTCTCATGAGGAATAGCATCTTCCAGCTC
ATGATACACACCCTTGACCCACTCGCTGAAGAACTTGGATCATATGGAAAACTAAAATATTATGACACAA
TGACTGAAGAAGGGAGGTTCAGAGAAAAAGCTTCAATTCTCCACAAGATCGCCAAGAAGAAATGCCAAGT
GGATGAGAATGAGAGGCAGAATGGGGCTGCCAATCATGTGGAAAAAATTGAACTCCCCAACAGCACAAGC
ACAGAGGTGGAAATGACGCCATCGAGCGAAGCTTCGGAACCCGTCCAGAATGGAAACCTCTCCCACAACA
TTGAAGCTGCAGATGCCCCGAAGGCCACAGAGACTGCCGAGGAAGAAGATGACCAGCCCCTCAGCTTGTC
CTGGCCGACCAACACCCGCAAGCAAGCCACCTTCCTAATCGTTTTTCCCATTGTGTTCCCTCTCTGGATC
ACCTTACCTGACGTCCGAAAACCTGCTTCCAGGAAGTTCTTCCCTATCACATTCTTCGGCTCCATCACCT
GGATTGCAGTTTTCTCATACCTGATGGTGTGGTGGGCACACCAGGTTGGAGAGACAATCGGCATTAGTGA
AGAAATCATGGGTCTGACCATCTTGGCTGCTGGGACCTCTATCCCTGATCTTATCACCAGTGTCATAGTG
GCCCGAAAGGGCCTGGGGGACATGGCTGTGTCCAGCTCTGTTGGAAGCAACATCTTTGACATCACTGTGG
GGCTCCCACTGCCCTGGCTCCTCTACACCATCATCCACAGATTCAGCCCAGTGACTGTCAGCAGCAATGG
CCTCTTCTGTGCCATCGTCCTTCTCTTCATCATGTTGCTTTTTGTCATTCTCTCCATCGCCCTCTGCAAA
TGGCGGATGAACAAAATCCTCGGCTTCATCATGTTTGGCCTCTACTTCGTGTTCCTGGTGGTCAGCGTCC
TCCTGGAAGACAAAGTTCTCGTGTGCCCTGTCTCCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR215153 representing NM_172426
Red=Cloning site Green=Tags(s)

MDLHQSPTARLLQKWCSHESPFGCRRHYNSRKKLKLIRVIGLVMGLVAVSTVPFSISAFTETDSQSNRGE
ASDMSGPRVAQGHRQRTLLDLNDKIRDYTPQPPASQEDQAENSTEHTQGDYPKDIFSLEERRKGAIILHV
IGMIYMFIALAIVCDEFFVPSLTVITEKLGISDDVAGATFMAAGGSAPELFTSLIGVFIAHSNVGIGTIV
GSAVFNILFVIGMCALFSREILNLTWWPLFRDVSFYIVDLLMLITFFLDNVIMWWESLLLLTAYFAYVVF
MKFNVQVERWVKQMISRNNVIKVTVPEAQAKSPTAGDKDGPTLPSKPRLQRGGSSASLHNSLMRNSIFQL
MIHTLDPLAEELGSYGKLKYYDTMTEEGRFREKASILHKIAKKKCQVDENERQNGAANHVEKIELPNSTS
TEVEMTPSSEASEPVQNGNLSHNIEAADAPKATETAEEEDDQPLSLSWPTNTRKQATFLIVFPIVFPLWI
TLPDVRKPASRKFFPITFFGSITWIAVFSYLMVWWAHQVGETIGISEEIMGLTILAAGTSIPDLITSVIV
ARKGLGDMAVSSSVGSNIFDITVGLPLPWLLYTIIHRFSPVTVSSNGLFCAIVLLFIMLLFVILSIALCK
WRMNKILGFIMFGLYFVFLVVSVLLEDKVLVCPVSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_172426
ORF Size 1998 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172426.2, NP_766014.1
RefSeq Size 10606 bp
RefSeq ORF 2001 bp
Locus ID 76376
Cytogenetics 4 C4
MW 74.7 kDa
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Citations

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