Olfr827 (NM_146300) Mouse Tagged ORF Clone

SKU MR213132

Olfr827 (Myc-DDK-tagged) - Mouse olfactory receptor 827 (Olfr827)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Olfr827
• Synonyms: MOR210-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213132 representing NM_146300; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGTGACAAGGAGAGAGACAACCACTCAGAAGTAACTGACTTCATTCACCACTCAGAAGTGACTGACT; TCATTCTTGTAGGCATCAGAGTCCGTCCAGAGCTCCACAGTGTCCTTTTTCTTCTATTTCTTATTATTTA; TGGGATGGTTCTTCTGGGAAACCTTAGCATGATTGGCATCATAGTGACTGATCCCAGGCTGAACACACCA; ATGTATTTCTTCCTAGGCAACCTCTCCGTGATTGACCTCTCCTACTCCACGGTTATTGTACCTAAAGCCA; TGGTAAACATCTTATCTCAGAAAAAGACCATATCCTTTGTGGGTTGTGCAGCTCAACTTTTCCTTTATGC; ACTTTTCATGGTAACAGAAGCCTTTATACTAGCAGCCATGGCCTATGACCGCTTCATTGCCATCTGCAAT; CCTCTCCTTTACAGCGTTCGAATGTCAAGGAGTGTCTGTGTCCAGTTGGTGGCTGGTTCCTATCTCTGCG; GCTGGGTCAGTTCCATCCTCCAAATCAGTGTAACATTCTCCATGTCTTTTTGTGCCTCTAGGGTCATTGA; TCACTTCTACTGTGATTCTAATCCAATCGAAAAGATCTCCTGTTCTAATACTTTTATCAATAAGATGGTG; TCACTTAGTCTGGCTGTGCTTATTATTTTGCCAACAATAATAGTTATTGTAGTATCTTACATGTATATTG; TATCCACAGTTTTAAAGATACATTCCAGTGAAGGGAGAAAAAAAGCCTTTTCTACCTGCAGCTCCCACTT; GGGGGTTGTAAGTTTGCTTTATGGGACTGTTTCCTTTGTGTATCTCACACCTCCAAATAATCCTGAACTT; CGTAAAATTGCTTCAGTATGTTATATTTTGTTCACACCTATGTTGAATCCCCTAATCTATTCTTTACGAA; ATAAGGATGTAAAAGATGCCATGAAAAAAGTCTTGTGCAAGCAAAAGGTTTTACTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213132 representing NM_146300; Red=Cloning site Green=Tags(s); ; MGDKERDNHSEVTDFIHHSEVTDFILVGIRVRPELHSVLFLLFLIIYGMVLLGNLSMIGIIVTDPRLNTP; MYFFLGNLSVIDLSYSTVIVPKAMVNILSQKKTISFVGCAAQLFLYALFMVTEAFILAAMAYDRFIAICN; PLLYSVRMSRSVCVQLVAGSYLCGWVSSILQISVTFSMSFCASRVIDHFYCDSNPIEKISCSNTFINKMV; SLSLAVLIILPTIIVIVVSYMYIVSTVLKIHSSEGRKKAFSTCSSHLGVVSLLYGTVSFVYLTPPNNPEL; RKIASVCYILFTPMLNPLIYSLRNKDVKDAMKKVLCKQKVLL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146300
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_146300.1, NP_666412.1
• RefSeq Size: 969 bp
• RefSeq ORF: 969 bp
• Locus ID: 258297
• Cytogenetics: 10 D3
• MW: 36 kDa
• Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]