Nat10 (NM_153126) Mouse Tagged ORF Clone

SKU MR211470

Nat10 (Myc-DDK-tagged) - Mouse N-acetyltransferase 10 (Nat10)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Nat10
• Synonyms: AI429152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211470 representing NM_153126; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATCGGAAGAAGGTGGATAACCGAATTCGAATTCTCATTGAGAATGGCGTAGCTGAGCGGCAGAGGT; CTCTTTTTGTTGTAGTTGGGGATCGAGGAAAAGATCAGGTGGTTATTCTTCATCATATGTTGTCCAAGGC; AACTGTGAAGGCTCGGCCCTCAGTCCTGTGGTGTTATAAGAAAGAGCTGGGATTTAGCAGTCACCGGAAG; AAGAGGATGCGGCAGCTACAGAAGAAAATAAAGAGTGGGACCTTGAACTTAAAGCAAGATGACCCCTTTG; AGCTTTTTGTAGCAGCCACAAACATTCGCTACTGCTACTACAATGAAACCCACAAGATTCTGGGCAATAC; TTTCGGCATGTGTGTCCTCCAGGATTTTGAAGCGTTAACTCCGAACTTGTTGGCCAGAACTGTAGAAACA; GTAGAAGGTGGTGGACTGGTGGTCATCCTCCTGCGGACCATGAACTCGCTTAAGCAGCTGTACACGATGA; CTATGGATGTGCATTCCAGGTACAGGACTGAGGCCCATCAGGACGTGGTGGGAAGATTTAACGAGAGGTT; TATTCTCTCTCTGGCCTCTTGTAAGAAGTGTCTGGTCATTGACGATCAGCTCGACATCCTGCCCATCTCC; TCCCACGTGGCCAGCATTGAAGCCTTACCTCCTCAGGCCCCGGATGAGAATCTCAGTCCTGCTGCTCTGG; AGCTGCTGGAGTTGAAAGAGAGCTTGCAGGACACTCAGCCCGTGGGTGTTCTGGTGGACTGCTGCAAGAC; CCTGGACCAGGCCAAAGCTGTCTTGAAATTCATTGAGGGGATCTCGGAGAAGACTCTAAGGAGTACTGTA; GCCCTCACCGCTGCCCGAGGAAGGGGCAAGTCTGCGGCCCTGGGCCTGGCTATCGCTGGAGCAGTGGCAT; TCGGGTATTCCAATATTTTTGTTACCTCCCCAAGCCCGGATAACCTCCACACGCTGTTTGAATTTGTATT; TAAAGGATTTGATGCTCTGCAGTATCAGGAGCATCTGGATTATGAGATTGTACAGTCGCTGAACCCCGAG; TTTAATAAAGCGGTGATCAGGGTCAATGTGTTCCGAGAGCACAGACAGACTATTCAGTACATCCACCCTG; CAGATGCTGTGAAACTGGGCCAGGCTGAGCTGGTTGTGATAGATGAAGCTGCCGCTATTCCCCTCCCCCT; GGTGAAGAGCCTGCTTGGGCCCTACCTGGTTTTCATGGCATCTACTATCAATGGCTACGAGGGCACTGGC; CGGTCACTGTCCCTCAAGCTCATTCAACAACTCCGTCAGCAGAGTGCCCAGAGCCAGGTCAGCACCACTG; CTGAGAACAAGACCACAACGACAGCCAGACTGGCCTCAGCTCGAACCTTGCATGAGGTTTCCCTCCAAGA; GTCAATCCGATACGCCCCTGGGGATGCAGTGGAGAAGTGGCTTAATGACCTGCTGTGCCTGGATTGCCTC; AACATCACCCGCATCGTTTCCGGCTGCCCCTTGCCTGAGGCCTGTGAGCTCTACTATGTTAACAGAGATA; CCCTCTTTTGCTACCACAAGGCCTCTGAAGTTTTCCTCCAGCGGCTCATGGCTCTCTATGTGGCTTCTCA; TTACAAGAACTCTCCCAACGACCTGCAGATGCTCTCAGACGCTCCTGCTCACCACCTCTTCTGCCTCCTG; CCACCTGTGCCCCCCACCCAGAATGCCCTGCCTGAAGTGCTTGCAGTTGTCCAGGTGTGCCTTGAGGGAG; AGATTTCTCGTCAGTCCATTTTGAATAGCCTCTCTCGAGGCAAGAAGGCTTCTGGGGACCTGATTCCATG; GACAGTGTCGGAACAGTTTCAAGATCCAGACTTTGGAGGCCTTTCTGGTGGACGGGTTGTTCGAATTGCT; GTTCACCCAGATTATCAAGGGATGGGATATGGCAGCCGAGCGCTGCAGCTGCTGCAGATGTATTATGAAG; GCAAGTTCCCTTGTCTGGAGGAAAAAGTCTTGGAGACACCACAAGAAATCCGCACCGTGAGCAGTGAGGC; TGTCAGCTTGTTGGAAGAAGTTATTACTCCCCGGAAGGATCTGCCCCCCTTGCTCCTCAAGTTGAATGAG; AGGCCTGCAGAGCGCCTGGATTACCTGGGGGTTTCCTATGGGCTGACCCCCAGGCTTCTCAAGTTCTGGA; AACGAGCTGGATTTGTTCCTGTCTATTTGAGACAGACCCCAAACGACCTGACTGGGGAACACTCGTGCAT; TATGCTGAAGACGCTGGCCGATGAGGATGAGGCTGAGCAGGGAGCTTGGCTGGCAGCATTTTGGAAAGAT; TTCCGAAGACGGTTCCTGGCCTTGCTGTCATACCAGTTCAGCACCTTCTCTCCTGCCCTGTCTCTGAACA; TCATTCAGAACAGGAACGTAGCCAAGTCAGCCCTGCCAGCCCTAGGCCGGGAGCATCTGGAGGCACTTTT; CCTTCCCTATGACCTGAAGCGTCTAGAGATGTACTCTCGGAACATGGTCGATTACCACCTCATCATGGAT; CTTATCCCAGCCATTTCCCGCTTGTACTTCCTGAACCAGCTAGGAGACCTGTCCCTGTCGGCTGCCCAGT; CGGCTCTTCTCTTGGGAATTGGCCTACAGCACAAGTCTGTGGATCAGCTGGAAAAGGAGATTGAGCTGCC; CTCAGGCCAGTTGATGGGGCTTTTCAACCGGATCATCCGCAAAGTTGTGAAGCTGTTCAATGATGTGCAG; GAAAAGGCTATTGAGGAGCAGATGGTGGCAGTGAAGGATGTGGTCATGGAGCCCACTATGAAGACCCTGA; GTGATGACCTGGATGAAGCAGCAAAGGAATTCCAGGAGAAACACAAGAAGGAAGTCGGGAAGCTGAAGGA; CATGGACCTCTCTCAATATGTAATTCGTGGGGACGATGAAGAGTGGAATGAAGTTTTGAGCAAAGCAGGG; CAGAATGCCTCCATTGTTAGTTTGAAAAGTGACAAGAAAAGGAAACTGGAAACAAAACAAGAACCCAAAC; AGAGCAAGAAGTTGAAGAAGAGAGATAACAACAGAAAGGATATGAAACTGAAGCGAAAGAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211470 representing NM_153126; Red=Cloning site Green=Tags(s); ; MNRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK; KRMRQLQKKIKSGTLNLKQDDPFELFVAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET; VEGGGLVVILLRTMNSLKQLYTMTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLDILPIS; SHVASIEALPPQAPDENLSPAALELLELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV; ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIVQSLNPE; FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG; RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL; NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL; PPVPPTQNALPEVLAVVQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA; VHPDYQGMGYGSRALQLLQMYYEGKFPCLEEKVLETPQEIRTVSSEAVSLLEEVITPRKDLPPLLLKLNE; RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLADEDEAEQGAWLAAFWKD; FRRRFLALLSYQFSTFSPALSLNIIQNRNVAKSALPALGREHLEALFLPYDLKRLEMYSRNMVDYHLIMD; LIPAISRLYFLNQLGDLSLSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNDVQ; EKAIEEQMVAVKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKDMDLSQYVIRGDDEEWNEVLSKAG; QNASIVSLKSDKKRKLETKQEPKQSKKLKKRDNNRKDMKLKRKK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153126
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153126.3
• RefSeq Size: 3856 bp
• RefSeq ORF: 3075 bp
• Locus ID: 98956
• UniProt ID: Q8K224
• Cytogenetics: 2 E2
• MW: 115.9 kDa
• Summary: RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA (By similarity). May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (By similarity). Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization (By similarity).[UniProtKB/Swiss-Prot Function]