L3mbtl3 (BC082309) Mouse Tagged Lenti ORF Clone

SKU
MR210974L3
Lenti ORF clone of L3mbtl3 (Myc-DDK-tagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672)
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,086.00
3 Weeks*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol L3mbtl3
Synonyms AI481284; MBT-1
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR210974).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN BC082309
ORF Size 2574 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC082309, AAH82309
RefSeq Size 4280 bp
RefSeq ORF 2576 bp
Locus ID 237339
Cytogenetics 10 A4
Summary Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility (By similarity). Required for normal maturation of myeloid progenitor cells.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
MC220678 L3mbtl3 (untagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672), (10ug) 10 ug
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MR210974 L3mbtl3 (Myc-DDK-tagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672) 10 ug
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MR210974L4 Lenti ORF clone of L3mbtl3 (mGFP-tagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.