Zw10 (NM_012039) Mouse Tagged ORF Clone

SKU MR210603

Zw10 (Myc-DDK-tagged) - Mouse ZW10 homolog (Drosophila), centromere/kinetochore protein (Zw10)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Zw10
• Synonyms: 6330566F14Rik; MmZw10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210603 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTCTTTCGTGACAGAAGTCTTGGCCCACTCGGGGAGCCTGGAGAAGGAGGATCTGGGCACCAGGA; TCAGCCGCCTGACCCGGCGGGTGGAGGAGATCAAGGGTGAAGTATGCAATATGATCAGCAAGAAGTACAG; TGAGTTCCTGCCTACCATGCAGAGTGCGCAGGCCCTAGTTACCCAGGTGGACACTCTATCTAACGACATC; GACCAGCTGAAATCCAGGATAGAGACTGAGGTCTGTCGGGATCTTCACATATCAACTGTTGAGTTTACAA; ACCTGAAGCAGCAGCTGGAAAGAGACTCTGTAGTCCTAACTCTGCTCAAACAACTCCAAGAGTTTTCCTC; TGCTATTGAAGAGTATAACAGTGCGTTAGCAGAGAAGAAGTACATCCCTGCTGCTCGGCACCTGGAGGAG; GCTCAGGAGTGCTTGAAGTTATTAAAGTCCAGAAAATGCTTTGACTTAAAAATGTTGAAGTCTCTCAGCA; TGGAGCTCACCGTGCAGAAGCAGAACATACTGTACCACCTGGGAGAAGACTGGCAGAAGCTGGTTGTATG; GAAGTTTCCGCCGGCAAAAGATACCAGCAGCTTAGAGTCTTGTCTACAAACAGAGCTTCATTTATGCACT; GAGCAGCCGGAGAAAGAGGACATGACTCCTCTGCCGTCCATCAGTTCTGTCCTCTTGGCCTTTTCCATTC; TTGGAGAATTACCCACCAAGCTGAAGTCATTTGGTCAGATGCTGCTGAAGTATATCCTTAAGCCTCTGGT; GACCTGCCCGTCGCTCCATGCTGTGATTGAAAGGCAGCCAAGTTCAGTTAGCATTTGTTTCGAGTCTCTG; ACGACGGACTTGGAACACCCATCACCACCCGAAGCTTTTGCAAAGATCCGATTGGTCCTGGAAGTGCTCC; AGAAACAGCTTCTAGATTTGCCTCTTGATGCTGACCTAGAAATTGGAAAAGTCCCTGGGATCGTGTTGGC; TGAGATGCTTGGTGAGGGGATCTGGGAGGACCTTTCTGAGTGCCTCATCAGAAACTGCTTGGTTTACTCT; ATTCCCACAAACAGCAGCAAGCTCCAGGAATACGAAGAGATTATACAGTCCACAGAAGAATTTGAAAAGT; TCCTAAAGGAGATGAGGTTTTTAAAAGGCGATACTACTGACTTGCTGAAATATGCTCGCAATATCAATTC; TCATTTTGCTAATAAGAAGTGCCAGGATGTGATTGTGGCGGCTAGAAATCTCATGACCTCTGAGATTCAC; AACACTGTCAAGATTGGTCCCGATTGTAAGGAAGCCTTACCAGACTTACCCAGTCCTGATGCTGATCACA; AGCTACAAGTACAGACGGTCTGCAAAGCGCAGTTCACCGACGCCGGGAACCTAGAGCCTGAGACTTCTCT; GGACCCTCAGTCCTTTTCTTTACCCACGTGCCGCATCAGCGAGGCTGTGAAGAAGTTAATGGAGCTCGCT; TATCAGACTTTACTTGAGGCAACAACAAGTAGCGACCAATGTGCTGTCCAGCTCTTCTACTCAGTGAGGA; ATATCTTCCATTTGTTCCATGATGTGGTACCAACATACCACAAGGAGAACCTTCGGAAGCTCCCCCAGTT; GGCTGCCATTCACCACAACAACTGCATGTATATTGCTCACCACTTGCTGACCCTCGGTCATCAGTTCAGA; TTGCGGCTCGCCCCCATCCTTTGCGATGGCACGACCACCTTTGTGGATCTTGTACCTGGCTTCAGAAGAC; TTGGGACGGAGTGCTTTTTGGCCCAAATGCAGGCACAGAAAGGAGAACTTCTGGAGAGATTGTCAAGTGC; TAGAAGCTTTGCGAACATGGACGACGAAGAGAATTACTCTGCAGCGAGCAAGGCAGTGAGGCAGGTGCTG; CACCAGCTAAGGAGACTTGGGATCGTGTGGCAGGATGTCCTGCCAGTGAACATATACTGCAAGGCCATGG; GGACCTTGCTGAACACAGCGATTGCTGAGATGATGAGCAGGATCACTGCTCTAGAGGACATCTCGACTGA; AGATGGTGATAGATTGTATTCCCTGTGCAAAACAGTGATGGATGAAGGTCCACAAGTGTTTGCACCTCTG; TCTGATGAAAACAAAAACAAGAAATACCAAGAAGAGGTTCCCGTCTATGTGTCCAAATGGATGCCTTTCA; AAGAACTCATGATAATGCTACAAGCTAGCTTGCAAGAAATTGGAGATCGGTGGGCAGATGGAAAAGGGCC; CCTGGCAACTGCATTCCCTTCCAGCGAAGTCAAAGCTTTAATCCGTGCCTTGTTCCAGAACACAGAAAGA; AGAGCAGCTGCCCTTGCTAAGATTAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210603 protein sequence; Red=Cloning site Green=Tags(s); ; MASFVTEVLAHSGSLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPTMQSAQALVTQVDTLSNDI; DQLKSRIETEVCRDLHISTVEFTNLKQQLERDSVVLTLLKQLQEFSSAIEEYNSALAEKKYIPAARHLEE; AQECLKLLKSRKCFDLKMLKSLSMELTVQKQNILYHLGEDWQKLVVWKFPPAKDTSSLESCLQTELHLCT; EQPEKEDMTPLPSISSVLLAFSILGELPTKLKSFGQMLLKYILKPLVTCPSLHAVIERQPSSVSICFESL; TTDLEHPSPPEAFAKIRLVLEVLQKQLLDLPLDADLEIGKVPGIVLAEMLGEGIWEDLSECLIRNCLVYS; IPTNSSKLQEYEEIIQSTEEFEKFLKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIH; NTVKIGPDCKEALPDLPSPDADHKLQVQTVCKAQFTDAGNLEPETSLDPQSFSLPTCRISEAVKKLMELA; YQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLRKLPQLAAIHHNNCMYIAHHLLTLGHQFR; LRLAPILCDGTTTFVDLVPGFRRLGTECFLAQMQAQKGELLERLSSARSFANMDDEENYSAASKAVRQVL; HQLRRLGIVWQDVLPVNIYCKAMGTLLNTAIAEMMSRITALEDISTEDGDRLYSLCKTVMDEGPQVFAPL; SDENKNKKYQEEVPVYVSKWMPFKELMIMLQASLQEIGDRWADGKGPLATAFPSSEVKALIRALFQNTER; RAAALAKIK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012039
• ORF Size: 2337 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012039.2
• RefSeq Size: 2917 bp
• RefSeq ORF: 2340 bp
• Locus ID: 26951
• UniProt ID: O54692
• Cytogenetics: 9 A5.3
• MW: 88.1 kDa
• Summary: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).[UniProtKB/Swiss-Prot Function]