Mre11a (NM_018736) Mouse Tagged ORF Clone

SKU MR210160

Mre11a (Myc-DDK-tagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (Mre11a)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Mre11a
• Synonyms: Mre11; Mre11b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210160 representing NM_018736; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCCCCACAGATCCACTTGACGATGAAGACACATTTAAAATCCTGGTTGCCACTGATATTCATCTTG; GATTTATGGAGAAAGATGCAGTTAGAGGAAATGATACATTTGTGACATTTGATGAAATCTTAAGACTTGC; CCTGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTCCATGAAAACAAGCCCTCGAGGAAA; ACCCTGCACAGCTGCTTGGAGCTGCTTAGGAAGTACTGTATGGGTGACCGCCCTGTGCAGTTTGAGGTCA; TCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTACCAGGATGGCAATCTCAA; CATTTCCATTCCAGTATTTAGTATCCACGGCAACCATGATGATCCCACGGGGGCAGATGCCCTCTGTGCC; CTGGATGTTTTAAGCTGTGCTGGGTTTGTGAATCACTTTGGACGGTCAATGTCGGTGGAGAAGGTTGACA; TTAGTCCGGTTCTGCTGCAGAAAGGAAGCACAAAACTCGCTCTGTACGGCTTAGGCTCCATTCCAGATGA; AAGGCTCTATCGGATGTTTGTGAATAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCATGG; TTTAACTTATTTGTGATTCATCAGAACAGGAGTAAGCATGGAAACACCAACTTCATTCCAGAGCAGTTTT; TGGATGACTTCATCGACCTCGTTATCTGGGGCCATGAACATGAGTGTAAAATTGGCCCAATCAAAAATGA; GCAGCAGCTCTTCTATGTGTCTCAGCCCGGAAGCTCAGTGGTGACGTCCCTTTCCCCTGGAGAAGCTGTG; AAGAAACATGTAGGCTTGCTGCGCATTAAAGGGAGAAAGATGAACATGCAGAAGCTGCCTCTCCGCACCG; TGCGGCGGTTCTTCATAGAAGACGTGGTTCTGGCTAACCACCCAAACCTGTTCAACCCTGACAATCCTAA; GGTGACCCAGGCCATCCAGAGCTTCTGTCTGGAGAAGATTGAAGAAATGCTTGACAGTGCTGAGCGGGAA; CGACTGGGGAATCCTCAGCAGCCGGGGAAGCCTCTTATCCGACTACGGGTGGACTATAGTGGAGGCTTTG; AACCTTTCAACGTTCTTCGTTTTAGCCAGAAGTTTGTGGATCGAGTCGCTAACCCAAAAGATGTCATCCA; CTTTTTCAGGCACAGGGAACAAAAGGGAAAAACAGGAGAAGAGATCAACTTTGGGATGCTCATCACAAAA; CCTGCTTCAGAAGGAGCAACGCTTAGAGTAGAAGACCTCGTAAAGCAGTATTTCCAAACTGCAGAGAAGA; ATGTTCAGCTCTCGCTGCTGACAGAAAGAGGGATGGGCGAAGCAGTTCAAGAGTTTGTGGACAAGGAAGA; GAAAGATGCCATCGAGGAATTAGTGAAGTACCAGCTGGAGAAAACACAGCGGTTTCTTAAGGAGCGCCAT; ATTGATGCTCTGGAAGACAAGATTGATGAGGAGGTCCGACGTTTCCGAGAAAGCAGACAGAGAAATACCA; ACGAAGAAGATGATGAAGTTCGAGAGGCCATGAGCAGAGCCCGGGCCCTCAGATCACAGTCAGAGACCTC; CACCTCAGCCTTTAGTGCTGAGGACCTGAGCTTTGATACATCGGAGCAGACAGCAAATGACTCTGATGAC; AGCCTGTCAGCAGTGCCGAGCAGAGGCCGAGGCCGAGGCCGAGGGCGAAGAGGAGCCAGAGGGCAGAGCT; CGGCACCTAGAGGAGGCTCTCAGAGAGGCCGAGACACTGGGCTGGAGATCACTACTCGAGGCAGGAGCTC; AAAGGCCACCTCATCAACATCTAGAAACATGTCCATTATAGACGCTTTCAGATCTACCCGACAACAGCCT; TCTAGAAATGTAGCCCCTAAGAATTACTCAGAGACCATTGAGGTGGATGACTCTGACGAAGATGACATTT; TTCCTACCAATTCCAGGGCTGATCAAAGGTGGTCGGGCACAACATCTAGCAAACGGATGTCCCAGAGCCA; GACAGCCAAAGGGGTAGACTTTGAATCAGATGAGGATGATGACGATGACCCTTTCATGAGCAGTAGTTGC; CCAAGAAGAAACCGAAGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210160 representing NM_018736; Red=Cloning site Green=Tags(s); ; MSPTDPLDDEDTFKILVATDIHLGFMEKDAVRGNDTFVTFDEILRLALENEVDFILLGGDLFHENKPSRK; TLHSCLELLRKYCMGDRPVQFEVISDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCA; LDVLSCAGFVNHFGRSMSVEKVDISPVLLQKGSTKLALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSW; FNLFVIHQNRSKHGNTNFIPEQFLDDFIDLVIWGHEHECKIGPIKNEQQLFYVSQPGSSVVTSLSPGEAV; KKHVGLLRIKGRKMNMQKLPLRTVRRFFIEDVVLANHPNLFNPDNPKVTQAIQSFCLEKIEEMLDSAERE; RLGNPQQPGKPLIRLRVDYSGGFEPFNVLRFSQKFVDRVANPKDVIHFFRHREQKGKTGEEINFGMLITK; PASEGATLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERH; IDALEDKIDEEVRRFRESRQRNTNEEDDEVREAMSRARALRSQSETSTSAFSAEDLSFDTSEQTANDSDD; SLSAVPSRGRGRGRGRRGARGQSSAPRGGSQRGRDTGLEITTRGRSSKATSSTSRNMSIIDAFRSTRQQP; SRNVAPKNYSETIEVDDSDEDDIFPTNSRADQRWSGTTSSKRMSQSQTAKGVDFESDEDDDDDPFMSSSC; PRRNRR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018736
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018736.3
• RefSeq Size: 3034 bp
• RefSeq ORF: 2121 bp
• Locus ID: 17535
• UniProt ID: Q61216
• Cytogenetics: 9 A2
• MW: 80.7 kDa
• Summary: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.[UniProtKB/Swiss-Prot Function]