Etfdh (NM_025794) Mouse Tagged ORF Clone

SKU
MR209442
Etfdh (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, dehydrogenase (Etfdh)
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$289.00 MSRP $574.00 MSRP $574.00
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Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Etfdh
Synonyms 0610010I20Rik; AV001013
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209442 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGTGCGACTAACCAAGCTGTCCTGCCCTGCGTATCACTGGTTTCATGCCTTAAAAATTAAGAAGT
GCCTACCTCTGTGTGCTCCAAGATGTTCTTCAACCTCTGCTGTACCTCAGATTACCACTCACTACACTGT
TCATCCCCGGGAGAAAGACAAAAGATGGGAAGGAGTGAATATGGAGAGGTTTGCAGAAGAAGCAGATGTG
GTCATAGTTGGTGCCGGCCCAGCAGGACTCTCTGCAGCTATTCGGCTAAAGCAGCTGGCTGCTGAACAGG
GGAAGGACATCCGTGTGTGTCTGGTGGAGAAAGCTGCTCAGATCGGAGCACACACGCTCTCAGGGGCTTG
TCTTGATCCAGCTGCCTTCAAAGAGCTCTTCCCAGACTGGAAGGAGAAAGGGGCTCCACTTAATACTCCT
GTAACAGAAGACAGGTTTGCCATTTTAACAGAGAAACACAGAATTCCTGTGCCAATTCTTCCAGGTCTTC
CGATGAACAATCATGGCAATTACATCGTACGCCTAGGACATCTGGTGAGCTGGATGGGAGAACAGGCAGA
GGCTCTTGGAGTCGAAGTGTATCCTGGGTATGCTGCTGCTGAGGTCCTTTATCATGAAGATGGTAGTGTA
AAAGGAATCGCCACTAATGATGTAGGCATACAAAAGGATGGTGCACCAAAGACAACATTTGAGAGAGGAC
TGGAACTGCATGCCAAAGTCACAGTCTTTGCAGAGGGTTGCCATGGACACCTAGCCAAGCAGCTTTATAA
GAAATTTGATCTGAGGGCCAGCTGTGATGCACAGACTTACGGAATTGGTTTGAAGGAGTTATGGATTATT
GATGAAAAGAAGTGGAAACCTGGAAGAGTAGATCACACTGTTGGTTGGCCCTTGGACAGACATACTTATG
GAGGCTCTTTCCTTTACCACTTAAATGAAGGTGAACCTCTAGTAGCTGTTGGCTTTGTGGTTGGCCTCGA
CTATCAGAATCCGTACCTGAGTCCATTCCGAGAGTTCCAGAGGTGGAAGCATCATCCCAGCATCCAGCCA
ACCCTGGAAGGCGGGAAGAGGATAGCCTACGGAGCCCGAGCGCTCAATGAAGGCGGCTTGCAGTCCATAC
CAAAACTCACTTTTCCTGGTGGCTTACTAATTGGTTGTAGTCCTGGTTTCATGAATGTCCCCAAGATCAA
AGGTACCCACACGGCAATGAAAAGTGGAAGTTTGGCAGCAGAATCAATTTTTAAGCAACTAACTAGTGAA
AATCTCCAGTCAAAGACAACAGGACTCCATGTAACTGAATATGAGGACAATTTGAAGCAATCCTGGGTAT
GGAAAGAGCTACACGCTGTTCGAAATATAAGGCCATCTTGCCACGGGATACTGGGAGTATATGGAGGGAT
GATTTACACTGGGATATTTTACTGGATATTGAGAGGAATGGAGCCATGGACTCTAAAACATAAAGGCTCA
GACTCTGATCAGCTCAAACCAGCCAAGGATTGTACACCCATTGAGTATCCGAAACCTGATGGACAGATCA
GTTTTGACCTCTTATCCTCTGTGGCTTTGAGTGGTACTAATCATGAACATGACCAGCCAGCACATTTAAC
CTTAAAGGATGACAGCATACCTGTTAATAGAAACCTGTCAATATACGATGGGCCTGAGCAGCGATTCTGC
CCTGCAGGAGTTTATGAATTTGTTCCTTTGGAACAAGGTGATGGATTTCGATTACAGATAAATGCTCAGA
ACTGTGTGCACTGTAAAACATGTGATATCAAAGACCCAAGTCAAAATATTAACTGGGTGGTCCCAGAAGG
AGGAGGAGGGCCTGCTTACAATGGCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR209442 protein sequence
Red=Cloning site Green=Tags(s)

MLVRLTKLSCPAYHWFHALKIKKCLPLCAPRCSSTSAVPQITTHYTVHPREKDKRWEGVNMERFAEEADV
VIVGAGPAGLSAAIRLKQLAAEQGKDIRVCLVEKAAQIGAHTLSGACLDPAAFKELFPDWKEKGAPLNTP
VTEDRFAILTEKHRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLYHEDGSV
KGIATNDVGIQKDGAPKTTFERGLELHAKVTVFAEGCHGHLAKQLYKKFDLRASCDAQTYGIGLKELWII
DEKKWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVAVGFVVGLDYQNPYLSPFREFQRWKHHPSIQP
TLEGGKRIAYGARALNEGGLQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGSLAAESIFKQLTSE
NLQSKTTGLHVTEYEDNLKQSWVWKELHAVRNIRPSCHGILGVYGGMIYTGIFYWILRGMEPWTLKHKGS
DSDQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSIPVNRNLSIYDGPEQRFC
PAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_025794
ORF Size 1848 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025794.2, NP_080070.2
RefSeq Size 2281 bp
RefSeq ORF 1851 bp
Locus ID 66841
UniProt ID Q921G7
Cytogenetics 3 34.91 cM
MW 68.1 kDa
Summary Accepts electrons from ETF and reduces ubiquinone.[UniProtKB/Swiss-Prot Function]
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Citations

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