Men1 (NM_001168490) Mouse Tagged ORF Clone

SKU MR208819

Men1 (Myc-DDK-tagged) - Mouse multiple endocrine neoplasia 1 (Men1), transcript variant 4

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Men1
• Synonyms: AW045611
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208819 representing NM_001168490; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGGCTGAAGGCCGCCCAGAAGACGCTGTTCCCTCTGCGCTCTATCGACGACGTGGTGCGCCTGTTTG; CTGCAGAGCTGGGCCGAGAGGAGCCTGACCTGGTGCTCCTGTCCTTGGTCCTGGGCTTCGTGGAGCATTT; CCTGGCTGTCAACCGTGTCATCCCCACCAACGTGCCGGAGCTCACCTTCCAGCCCAGCCCCGCACCCGAC; CCTCCTGGTGGCCTCACCTACTTCCCGGTGGCCGACCTATCCATCATTGCTGCCCTCTATGCCCGATTCA; CCGCTCAGATCCGCGGCGCTGTGGACCTCTCCCTCTATCCTCGAGAGGGAGGTGTTTCTAGTCGCGAACT; GGTAAAAAAGGTCTCGGATGTCATATGGAACAGCCTCAGCCGCTCCTACTTCAAGGACCGGGCCCACATC; CAGTCCCTCTTCAGCTTCATCACAGGCACCAAACTGGACAGCTCGGGCGTGGCCTTTGCAGTGGTAGGGG; CCTGCCAGGCTCTGGGTCTCAGAGATGTCCATCTGGCCCTGTCTGAAGATCATGCTTGGGTGGTGTTTGG; GCCCAACGGGGAGCAGACAGCTGAGGTGACGTGGCACGGCAAAGGCAACGAGGACCGCAGAGGCCAGACA; GTCAATGCCGGTGTGGCTGAGCGGAGCTGGCTGTACCTGAAGGGGTCGTACATGCGCTGCGACCGTAAGA; TGGAGGTGGCGTTCATGGTGTGTGCCATCAACCCTTCCATCGATCTTCACACTGACTCTTTGGAACTGTT; GCAACTACAGCAGAAGCTGCTCTGGCTGCTGTATGACCTCGGACATCTGGAAAGATACCCCATGGCGCTA; GGGAACTTGGCAGACCTGGAGGAGCTGGAGCCTACCCCGGGCCGGCCAGACCCACTCACCCTTTATCACA; AGGGAATTGCCTCAGCTAAGACCTACTACCAGGATGAACACATCTACCCCTACATGTACCTGGCTGGCTA; CCACTGTCGCAACCGAAATGTGCGCGAAGCCCTGCAGGCCTGGGCCGACACTGCCACTGTTATCCAAGAC; TACAACTACTGCCGGGAGGATGAGGAGATCTACAAGGAATTCTTTGAAGTGGCCAATGACGTCATCCCCA; ACCTGCTGAAGGAGGCGGCCAGCCTGCTAGAAACAGGCGAAGAGCGGACTGGGGAGCAAGCCCAGGTGCG; GCAGAAGGTGCACATAGTTAGCCGGGAAGCAGAGGCGGCCGAGGCTGAAGAACCATGGGGGGATGAAGCC; CGAGAAGGCCGTCGGCGTGGTCCCCGAAGAGAGTCCAAGCCTGAGGAGCCACCACCACCCAAGAAGCCTG; CATTGGACAAGGGCCCGGGCTCAGGACAAAGTGCAGGGTCGGGACCACCTAGGAAAACGTCAGGGACTGT; CCCAGGTACTACCCGCGGTGGCCAGGAAGTCGGCAATGCTGCTCAGGCTCCAGCACCTGCAGCATCGCCA; CCCCCAGAGGGCCCAGTGCTCACTTTCCAGAGTGAGAAGATGAAAGGCATGAAGGAGCTACTGGTGGCCA; CCAAGATCAACTCGAGCGCCATCAAGCTGCAACTCACGGCACAGTCGCAAGTGCAGATGAAGAAACAGAA; AGTGTCCACACCCAGCGACTACACACTCTCTTTCCTAAAGCGGCAGCGCAAGGGCCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208819 representing NM_001168490; Red=Cloning site Green=Tags(s); ; MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPD; PPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHI; QSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQT; VNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMAL; GNLADLEELEPTPGRPDPLTLYHKGIASAKTYYQDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQD; YNYCREDEEIYKEFFEVANDVIPNLLKEAASLLETGEERTGEQAQVRQKVHIVSREAEAAEAEEPWGDEA; REGRRRGPRRESKPEEPPPPKKPALDKGPGSGQSAGSGPPRKTSGTVPGTTRGGQEVGNAAQAPAPAASP; PPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168490
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001168490.1, NP_001161962.1
• RefSeq Size: 2815 bp
• RefSeq ORF: 1671 bp
• Locus ID: 17283
• UniProt ID: O88559
• Cytogenetics: 19 A
• MW: 61.9 kDa
• Summary: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression (By similarity). May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair.[UniProtKB/Swiss-Prot Function]