Atp5a1 (NM_007505) Mouse Tagged ORF Clone

SKU MR208769

Atp5a1 (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (Atp5a1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Atp5a1
• Synonyms: AI035633; AL022851; AL023067; Atpm; D18Ertd206e; Mom2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208769 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTGTCTGTGCGCGTCGCCGCGGCCGTGGCCCGTGCCCTCCCTCGACGGGCGGGACTGGTCTCCAAAA; ATGCTTTGGGGTCATCCTTTGTTGGTGCAAGAAATCTCCATGCCTCTAACACTCGACTTCAGAAGACTGG; CACAGCTGAGATGTCCTCCATTCTCGAGGAGAGGATTCTGGGAGCTGACACGTCTGTTGACCTGGAGGAG; ACTGGGCGTGTGTTAAGCATTGGTGATGGTATTGCGCGAGTGCACGGGCTGAGGAATGTTCAAGCAGAGG; AGATGGTGGAATTCTCTTCAGGCTTAAAGGGTATGTCCCTGAACTTGGAACCCGACAATGTTGGAGTTGT; CGTGTTTGGGAATGACAAGCTAATTAAAGAAGGAGACGTTGTGAAGAGGACAGGCGCCATCGTGGATGTC; CCCGTTGGCGAGGAGCTGTTGGGCCGTGTGGTTGACGCCCTCGGTAATGCTATTGATGGAAAGGGTCCAA; TTGGTTCCAAGACCCGCAGACGAGTGGGCCTGAAAGCCCCTGGAATTATCCCCCGAATCTCTGTGCGGGA; GCCGATGCAGACCGGCATCAAGGCTGTGGATAGCCTGGTGCCCATTGGCCGGGGTCAGCGTGAGCTGATT; ATTGGTGACAGACAGACTGGGAAAACATCGATTGCCATTGACACAATCATCAACCAGAAACGTTTCAACG; ATGGGACCGACGAGAAGAAGAAGCTGTACTGCATCTACGTCGCGATTGGTCAGAAGCGGTCCACTGTGGC; TCAGCTGGTGAAGAGACTGACGGATGCGGATGCCATGAAGTACACCATCGTGGTGTCAGCCACTGCCTCT; GACGCTGCCCCGCTTCAGTACTTGGCTCCCTACTCCGGCTGCTCCATGGGAGAGTACTTTAGAGACAACG; GCAAGCACGCTTTGATCATCTATGACGATCTATCCAAGCAGGCTGTCGCTTACCGCCAGATGTCTCTGTT; GCTCCGCCGACCCCCTGGTCGTGAGGCCTATCCTGGTGATGTGTTCTACCTACACTCCCGCCTGCTGGAG; AGAGCAGCCAAGATGAACGATTCCTTTGGTGGTGGCTCTTTGACTGCCTTACCAGTCATTGAAACACAGG; CTGGTGATGTGTCCGCTTACATTCCAACAAATGTTATTTCCATCACTGACGGACAGATCTTCTTGGAAAC; AGAATTGTTCTATAAAGGCATCCGCCCTGCCATTAATGTGGGTTTGTCTGTGTCCCGTGTCGGATCTGCT; GCCCAAACCAGAGCCATGAAGCAGGTGGCAGGTACCATGAAGCTGGAGTTGGCTCAGTACCGGGAGGTGG; CTGCGTTTGCCCAGTTTGGTTCTGATTTGGACGCTGCCACTCAACAGCTCTTGAGTCGTGGTGTGCGTCT; GACCGAGTTGCTAAAGCAAGGACAGTACTCTCCCATGGCTATTGAAGAACAGGTGGCTGTTATCTATGCG; GGTGTACGGGGTTATCTTGACAAACTGGAGCCCAGCAAGATCACAAAGTTTGAGAATGCTTTCTTGTCTC; ACGTTATCAGCCAGCACCAGTCCCTCTTGGGCAATATCAGGTCTGATGGGAAAATCTCAGAACAGTCAGA; CGCAAAGCTCAAAGAGATTGTAACAAACTTCTTGGCTGGGTTTGAACCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208769 protein sequence; Red=Cloning site Green=Tags(s); ; MLSVRVAAAVARALPRRAGLVSKNALGSSFVGARNLHASNTRLQKTGTAEMSSILEERILGADTSVDLEE; TGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDVVKRTGAIVDV; PVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELI; IGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATAS; DAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLE; RAAKMNDSFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSA; AQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYA; GVRGYLDKLEPSKITKFENAFLSHVISQHQSLLGNIRSDGKISEQSDAKLKEIVTNFLAGFEP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007505
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007505.2, NP_031531.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 1662 bp
• Locus ID: 11946
• UniProt ID: Q03265
• Cytogenetics: 18 52.38 cM
• MW: 59.8 kDa
• Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (By similarity).[UniProtKB/Swiss-Prot Function]