Ppp5c (NM_011155) Mouse Tagged ORF Clone
CAT#: MR208000
- TrueORF®
Ppp5c (Myc-DDK-tagged) - Mouse protein phosphatase 5, catalytic subunit (Ppp5c)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011155" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ppp5c |
Synonyms | AU020526; PP5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208000 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGGCGGAGGGCGAGCGGACTGAGTGTGCTGAGACCCCCCGGGACGAACCCCCGGCCGATGGCG CTCTGAAGCGGGCAGAGGAGCTCAAGACACAGGCCAACGACTACTTCAAAGCCAAGGACTACGAGAACGC GATCAAGTTCTACAGTCAGGCCATCGAGTTGAACCCCGGCAATGCCATCTACTATGGCAACCGCAGCCTG GCCTACCTGCGCACTGAGTGCTATGGCTATGCACTGGGCGACGCCACACGGGCCATCGAGCTTGACAAGA AGTACATCAAAGGCTACTACCGCCGGGCGGCCAGCAACATGGCACTGGGCAAGTTCCGGGCTGCCCTGCG TGACTACGAGACGGTGGTGAAAGTGAAGCCTAATGACAAGGATGCCAAGATGAAGTACCAGGAGTGCAGC AAGATTGTGAAGCAGAAGGCCTTTGAGAGGGCCATTGCAGGTGACGAGCACAGACGCTCTGTCGTGGACT CTCTGGACATTGAAAGCATGACCATTGAAGATGAGTACAGCGGGCCCAAGCTTGAGGATGGCAAAGTGAC AATCACCTTCATGAAAGACCTCATGCAGTGGTACAAGGATCAGAAGAAACTGCACCGGAAGTGCGCCTAC CAGATCCTAGTACAGGTGAAAGAAGTCCTCTGCAAGCTGAGCACGCTGGTGGAGACGACGCTGAAAGAGA CAGAGAAGATTACAGTGTGCGGGGACACCCATGGCCAGTTCTACGACCTCCTCAACATATTTGAGCTCAA CGGTTTACCCTCAGAGACCAACCCCTATATATTTAATGGCGATTTTGTGGACCGTGGTTCCTTCTCCGTT GAAGTGATCCTCACCCTCTTCGGCTTTAAGCTCCTGTATCCAGATCATTTCCATCTACTTCGAGGCAACC ACGAGACAGACAACATGAACCAGATCTACGGGTTCGAGGGCGAGGTGAAGGCCAAGTACACAGCCCAGAT GTATGAGCTCTTCAGCGAGGTGTTCGAGTGGCTTCCGCTGGCGCAGTGTATCAATGGCAAAGTGCTGATC ATGCACGGAGGCCTATTCAGCGAAGATGGTGTCACTCTGGATGACATCCGAAAGATTGAGCGGAATCGGC AGCCCCCAGACTCAGGTCCCATGTGTGACCTGCTGTGGTCAGATCCCCAGCCACAGAATGGGCGCTCCGT CAGCAAGCGTGGTGTGAGTTGCCAGTTTGGGCCTGATGTCACCAAGGCCTTCCTGGAGGAGAATCAACTG GACTATATCATCCGCAGCCATGAAGTCAAAGCCGAGGGCTACGAGGTGGCCCATGGTGGCCGCTGTGTCA CTGTCTTTTCTGCCCCCAACTATTGTGACCAGATGGGAAACAAAGCCTCCTACATCCACCTCCAGGGCTC CGACCTGCGGCCCCAGTTCCACCAATTCACAGCAGTGCCTCACCCCAATGTCAAGCCCATGGCATACGCC AACACGCTTCTGCAGCTAGGAATGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208000 protein sequence
Red=Cloning site Green=Tags(s) MAMAEGERTECAETPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPGNAIYYGNRSL AYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPNDKDAKMKYQECS KIVKQKAFERAIAGDEHRRSVVDSLDIESMTIEDEYSGPKLEDGKVTITFMKDLMQWYKDQKKLHRKCAY QILVQVKEVLCKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSV EVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLI MHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSVSKRGVSCQFGPDVTKAFLEENQL DYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYA NTLLQLGMM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011155 |
ORF Size | 1500 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011155.1, NP_035285.1 |
RefSeq Size | 2084 bp |
RefSeq ORF | 1500 bp |
Locus ID | 19060 |
UniProt ID | Q60676 |
Cytogenetics | 7 9.15 cM |
MW | 56.8 kDa |
Gene Summary | Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. Dephosphorylates and may play a role in the regulation of TAU/MAPT. Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216972 | Ppp5c (untagged) - Mouse protein phosphatase 5, catalytic subunit (Ppp5c), (10ug) |
USD 503.00 |
|
MG208000 | Ppp5c (tGFP-tagged) - Mouse protein phosphatase 5, catalytic subunit (Ppp5c) |
USD 657.00 |
|
MR208000L3 | Lenti ORF clone of Ppp5c (Myc-DDK-tagged) - Mouse protein phosphatase 5, catalytic subunit (Ppp5c) |
USD 757.00 |
|
MR208000L4 | Lenti ORF clone of Ppp5c (mGFP-tagged) - Mouse protein phosphatase 5, catalytic subunit (Ppp5c) |
USD 757.00 |
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