Arih1 (NM_019927) Mouse Tagged ORF Clone

SKU MR207099

Arih1 (Myc-DDK-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Arih1
• Synonyms: Ari; Ari1; AU021774; Hari; Hhari; Ubch7bp; Uip77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207099 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGGAATGTATCCGGGAGGTCAACGAGGTCATCCAGAATCCAGCAACTATCACGAGAATACTCCTTA; GCCACTTCAATTGGGATAAAGAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGC; TGAGTGTCATGTAATTAATCCAAGTAAAAAGTCTCGAACTCGCCAGATGAATACAAGGTCATCGGCACAG; GATATGCCGTGTCAGATCTGCTACTTGAACTACCCTAACTCGTATTTTACTGGCCTTGAATGTGGACATA; AGTTTTGTATGCAGTGCTGGAGTGAATATTTAACTACAAAAATAATGGAGGAAGGCATGGGGCAGACTAT; TTCGTGTCCTGCGCATGGTTGTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCC; AAAGTTAAGTTAAAATATCAACATTTAATAACAAATAGCTTTGTAGAGTGCAACCGACTGTTAAAGTGGT; GTCCTGCCCCAGATTGCCACCATGTTGTTAAAGTCCAGTATCCTGATGCAAAACCAGTTCGCTGCAAATG; TGGCCGCCAGTTTTGCTTTAACTGTGGTGAAAATTGGCATGATCCAGTTAAATGTAAGTGGTTGAAGAAG; TGGATTAAAAAGTGTGATGATGACAGTGAAACTTCTAATTGGATTGCAGCTAACACAAAGGAATGTCCCA; AATGCCATGTCACAATTGAGAAGGATGGCGGTTGTAATCACATGGTCTGTCGTAACCAGAACTGTAAAGC; AGAATTTTGCTGGGTGTGTCTTGGCCCTTGGGAACCGCATGGATCTGCCTGGTACAACTGTAACCGCTAT; AATGAGGATGATGCAAAGGCAGCAAGAGATGCCCAAGAGCGATCTAGGGCGGCCCTGCAGAGATACCTGT; TCTACTGTAATCGCTATATGAACCACATGCAGAGTCTACGCTTCGAGCATAAGCTGTATGCTCAGGTGAA; ACAGAAGATGGAGGAGATGCAGCAGCACAACATGTCCTGGATCGAGGTGCAGTTCCTGAAGAAAGCAGTT; GATGTCCTCTGCCAGTGTCGTGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATA; ACCAGTCCATTATCTTTGAGAATAATCAAGCAGATCTAGAAAATGCCACAGAGGTGCTTTCGGGCTACCT; TGAACGAGATATTTCCCAAGATTCTCTGCAAGATATAAAGCAGAAAGTCCAAGATAAGTACAGATACTGT; GAGAGTCGACGAAGGGTTTTGTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTG; AGGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207099 protein sequence; Red=Cloning site Green=Tags(s); ; MVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQ; DMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDS; KVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKK; WIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRY; NEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAV; DVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYC; ESRRRVLLQHVHEGYEKDLWEYIED; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019927
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019927.1, NP_064311.1
• RefSeq Size: 6435 bp
• RefSeq ORF: 1668 bp
• Locus ID: 23806
• UniProt ID: Q9Z1K5
• Cytogenetics: 9 B
• MW: 52.7 kDa
• Summary: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2.[UniProtKB/Swiss-Prot Function]