Gpx6 (NM_145451) Mouse Tagged ORF Clone

SKU MR202525

Txnrd2 (Myc-DDK-tagged) - Mouse thioredoxin reductase 2 (cDNA clone MGC:5671 IMAGE:3584154), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Product Data

• Type: Mouse Untagged ORF Clone
• Target Symbol: Gpx6
• Synonyms: 1700020G18Rik; olfa; olfactory; Ry2d1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202525 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCCAGAAGTTGTGGGGTTCCTGTCTTTTCTCATTGTTTATGGCTGCCTTAGCTCAGGAGACTCTGA; ATCCTCAAAAATCGAAGGTGGATTGCAACAAAGGGGTGACTGGCACCGTCTATGAGTATGGAGCCAACAC; CATAGATGGTGGGGGGTTTGTCAACTTCCAGCAGTATGCAGGAAAGCACATCCTCTTTGTCAACGTGGCA; TCCTTCTGTGGCCTGACAGCTACGTACCCTGAGTTGAACACACTGCAGGAGGAGCTGAAGCCATTCAACG; TCACGGTTTTGGGCTTTCCGTGCAACCAGTTCGGAAAGCAAGAACCTGGAAAGAACTCAGAGATTCTCCT; TGGACTCAAGTATGTGCGTCCAGGCGGTGGCTATGTCCCCAATTTCCAGCTCTTTGAGAAGGGGGATGTG; AACGGAGACAATGAACAAAAGGTTTTTTCTTTCCTAAAGAACTCCTGCCCTCCCACCTCTGAACTTTTTG; GCTCTCCAGAACATCTCTTCTGGGATCCCATGAAGATTCATGATATCCGCTGGAACTTTGAGAAGTTCCT; GGTGGGACCTGATGGAGTCCCTGTCATGCGCTGGTTCCACCATACTCCTGTCAGAATTGTCCAGTCAGAC; ATCATGGAGTACCTAAACCAAACCAGTACCCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202525 protein sequence; Red=Cloning site Green=Tags(s); ; MAQKLWGSCLFSLFMAALAQETLNPQKSKVDCNKGVTGTVYEYGANTIDGGGFVNFQQYAGKHILFVNVA; SFCGLTATYPELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDV; NGDNEQKVFSFLKNSCPPTSELFGSPEHLFWDPMKIHDIRWNFEKFLVGPDGVPVMRWFHHTPVRIVQSD; IMEYLNQTSTQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145451
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145451.2
• RefSeq Size: 1262 bp
• RefSeq ORF: 666 bp
• Locus ID: 75512
• UniProt ID: Q91WR8
• Cytogenetics: 13 A3.1
• Summary: This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidases catalyze the reduction of a variety of hydroperoxides using glutathione as a specific electron donor substrate, and thereby protect cells against oxidative damage. Expression of this gene is restricted to embryos and adult olfactory epithelium. The mouse and rat orthologs contain a cysteine (Cys) residue at the active site, unlike the human counterpart, which is a selenoprotein, containing selenocysteine (Sec) instead. [provided by RefSeq, Jul 2017]