Atf3 (NM_007498) Mouse Tagged ORF Clone

SKU
MR201634
Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$450.00
In Stock*
Specifications
Product Data
Target Symbol Atf3
Synonyms LRG-21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCTTCAACATCCAGGCCAGGTCTCTGCCTCAGAAGTCAGTGCGACCGCCATTGTCCCCTGCCTCT
CACCTCCTGGGTCACTGGTATTTGAGGATTTTGCTAACCTGACACCCTTTGTCAAGGAAGAGCTGAGATT
CGCCATCCAGAATAAACACCTCTGCCATCGGATGTCCTCTGCGCTGGAGTCAGTTACCGTCAACAACAGA
CCCCTGGAGATGTCAGTCACCAAGTCTGAGGCGGCCCCTGAAGAAGATGAGAGGAAAAGGAGGCGGCGAG
AAAGAAATAAAATTGCTGCTGCCAAGTGTCGAAACAAGAAAAAGGAGAAGACAGAGTGCCTGCAGAAAGA
GTCAGAGAAACTGGAGAGTGTGAATGCTGAGCTGAAGGCCCAGATCGAGGAGCTGAAGAATGAGAAACAG
CATTTGATATACATGCTCAACCTGCACCGGCCCACCTGTATCGTCCGGGCTCAGAATGGACGGACACCGG
AAGACGAGAGGAACCTCTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201634 protein sequence
Red=Cloning site Green=Tags(s)

MMLQHPGQVSASEVSATAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVNNR
PLEMSVTKSEAAPEEDERKRRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQ
HLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_007498
ORF Size 543 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007498.2, NP_031524.2
RefSeq Size 1981 bp
RefSeq ORF 546 bp
Locus ID 11910
UniProt ID Q60765
Cytogenetics 1 96.28 cM
MW 20.7 kDa
Summary This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter (By similarity).[UniProtKB/Swiss-Prot Function]
Write Your Own Review
You're reviewing:Atf3 (NM_007498) Mouse Tagged ORF Clone
Your Rating
SKU Description Size Price
MC201919 Atf3 (untagged) - Mouse activating transcription factor 3 (Atf3), (10ug) 10 ug
$450.00
MG201634 Atf3 (tGFP-tagged) - Mouse activating transcription factor 3 (Atf3) 10 ug
$650.00
MR201634L1 Lenti ORF clone of Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3) 10 ug
$750.00
MR201634L2 Lenti ORF clone of Atf3 (mGFP-tagged) - Mouse activating transcription factor 3 (Atf3) 10 ug
$750.00
MR201634L3 Lenti ORF clone of Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3) 10 ug
$750.00
MR201634L4 Lenti ORF clone of Atf3 (mGFP-tagged) - Mouse activating transcription factor 3 (Atf3) 10 ug
$750.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.