Atf3 (NM_007498) Mouse Tagged ORF Clone
SKU
MR201634
Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3)
-
TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Target Symbol | Atf3 |
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Synonyms | LRG-21 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>MR201634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTTCAACATCCAGGCCAGGTCTCTGCCTCAGAAGTCAGTGCGACCGCCATTGTCCCCTGCCTCT CACCTCCTGGGTCACTGGTATTTGAGGATTTTGCTAACCTGACACCCTTTGTCAAGGAAGAGCTGAGATT CGCCATCCAGAATAAACACCTCTGCCATCGGATGTCCTCTGCGCTGGAGTCAGTTACCGTCAACAACAGA CCCCTGGAGATGTCAGTCACCAAGTCTGAGGCGGCCCCTGAAGAAGATGAGAGGAAAAGGAGGCGGCGAG AAAGAAATAAAATTGCTGCTGCCAAGTGTCGAAACAAGAAAAAGGAGAAGACAGAGTGCCTGCAGAAAGA GTCAGAGAAACTGGAGAGTGTGAATGCTGAGCTGAAGGCCCAGATCGAGGAGCTGAAGAATGAGAAACAG CATTTGATATACATGCTCAACCTGCACCGGCCCACCTGTATCGTCCGGGCTCAGAATGGACGGACACCGG AAGACGAGAGGAACCTCTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201634 protein sequence
Red=Cloning site Green=Tags(s) MMLQHPGQVSASEVSATAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVNNR PLEMSVTKSEAAPEEDERKRRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQ HLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007498 |
ORF Size | 543 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_007498.2, NP_031524.2 |
RefSeq Size | 1981 bp |
RefSeq ORF | 546 bp |
Locus ID | 11910 |
UniProt ID | Q60765 |
Cytogenetics | 1 96.28 cM |
MW | 20.7 kDa |
Summary | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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MC201919 | Atf3 (untagged) - Mouse activating transcription factor 3 (Atf3), (10ug) | 10 ug |
$450.00
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MG201634 | Atf3 (tGFP-tagged) - Mouse activating transcription factor 3 (Atf3) | 10 ug |
$650.00
|
|
MR201634L1 | Lenti ORF clone of Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3) | 10 ug |
$750.00
|
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MR201634L2 | Lenti ORF clone of Atf3 (mGFP-tagged) - Mouse activating transcription factor 3 (Atf3) | 10 ug |
$750.00
|
|
MR201634L3 | Lenti ORF clone of Atf3 (Myc-DDK-tagged) - Mouse activating transcription factor 3 (Atf3) | 10 ug |
$750.00
|
|
MR201634L4 | Lenti ORF clone of Atf3 (mGFP-tagged) - Mouse activating transcription factor 3 (Atf3) | 10 ug |
$750.00
|
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.