Il1r1 (NM_001123382) Mouse Tagged ORF Clone

SKU MG227513

Il1r1 (tGFP-tagged) - Mouse interleukin 1 receptor type I (Il1r1) transcript variant 2, (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Il1r1
• Synonyms: CD121a; CD121b; IL-1R1; IL-iR; Il1r-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227513 representing NM_001123382; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAAGTGCTACTGGGGCTCATTTGTCTCATGGTGCCTCTGCTGTCGCTGGAGATTGACGTATGTACAG; AATATCCAAATCAGATCGTTTTGTTTTTATCTGTAAATGAAATTGATATTCGCAAGTGTCCTCTTACTCC; AAATAAAATGCACGGCGACACCATAATTTGGTACAAGAATGACAGCAAGACCCCCATATCAGCGGACCGG; GACTCCAGGATTCATCAGCAGAATGAACATCTTTGGTTTGTACCTGCCAAGGTGGAGGACTCAGGATATT; ACTATTGTATAGTAAGAAACTCAACTTACTGCCTCAAAACTAAAGTAACCGTAACTGTGTTAGAGAATGA; CCCTGGCTTGTGTTACAGCACACAGGCCACCTTCCCACAGCGGCTCCACATTGCCGGGGATGGAAGTCTT; GTGTGCCCTTATGTGAGTTATTTTAAAGATGAAAATAATGAGTTACCCGAGGTCCAGTGGTATAAGAACT; GTAAACCTCTGCTTCTTGACAACGTGAGCTTCTTCGGAGTAAAAGATAAACTGTTGGTGAGGAATGTGGC; TGAAGAGCACAGAGGGGACTATATATGCCGTATGTCCTATACGTTCCGGGGGAAGCAATATCCGGTCACA; CGAGTAATACAATTTATCACAATAGATGAAAACAAGAGGGACAGACCTGTTATCCTGAGCCCTCGGAATG; AGACGATCGAAGCTGACCCAGGATCAATGATACAACTGATCTGCAACGTCACGGGCCAGTTCTCAGACCT; TGTCTACTGGAAGTGGAATGGATCAGAAATTGAATGGAATGATCCATTTCTAGCTGAAGACTATCAATTT; GTGGAACATCCTTCAACCAAAAGAAAATACACACTCATTACAACACTTAACATTTCAGAAGTTAAAAGCC; AGTTTTATCGCTATCCGTTTATCTGTGTTGTTAAGAACACAAATATTTTTGAGTCGGCGCATGTGCAGTT; AATATACCCAGTCCCTGACTTCAAGAATTACCTCATCGGGGGCTTTATCATCCTCACGGCTACAATTGTA; TGCTGTGTGTGCATCTATAAAGTCTTCAAGGTTGACATAGTGCTTTGGTACAGGGACTCCTGCTCTGGTT; TTCTTCCTTCAAAAGCTTCAGATGGAAAGACATACGATGCCTATATTCTTTATCCCAAGACCCTGGGAGA; GGGGTCCTTCTCAGACTTAGATACTTTTGTTTTTAAACTGTTGCCTGAGGTCTTGGAGGGACAGTTTGGA; TACAAGCTGTTCATTTATGGAAGGGATGACTATGTTGGAGAAGATACCATCGAGGTTACTAATGAAAATG; TAAAGAAAAGCAGGAGGCTGATTATCATTCTAGTGAGAGATATGGGAGGCTTCAGCTGGCTGGGCCAGTC; ATCTGAAGAGCAAATAGCCATATACAATGCTCTCATCCAGGAAGGAATTAAAATCGTCCTGCTTGAGTTG; GAGAAAATCCAAGACTATGAGAAAATGCCAGATTCTATTCAGTTCATTAAGCAGAAACACGGAGTCATTT; GCTGGTCAGGAGACTTTCAAGAAAGACCACAGTCTGCAAAGACCAGGTTCTGGAAAAACTTAAGATACCA; GATGCCAGCCCAACGGAGATCACCATTGTCTAAACACCGCTTACTAACCCTGGATCCTGTGCGGGACACT; AAGGAGAAACTGCCGGCAGCAACACACTTACCACTCGGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227513 representing NM_001123382; Red=Cloning site Green=Tags(s); ; MKVLLGLICLMVPLLSLEIDVCTEYPNQIVLFLSVNEIDIRKCPLTPNKMHGDTIIWYKNDSKTPISADR; DSRIHQQNEHLWFVPAKVEDSGYYYCIVRNSTYCLKTKVTVTVLENDPGLCYSTQATFPQRLHIAGDGSL; VCPYVSYFKDENNELPEVQWYKNCKPLLLDNVSFFGVKDKLLVRNVAEEHRGDYICRMSYTFRGKQYPVT; RVIQFITIDENKRDRPVILSPRNETIEADPGSMIQLICNVTGQFSDLVYWKWNGSEIEWNDPFLAEDYQF; VEHPSTKRKYTLITTLNISEVKSQFYRYPFICVVKNTNIFESAHVQLIYPVPDFKNYLIGGFIILTATIV; CCVCIYKVFKVDIVLWYRDSCSGFLPSKASDGKTYDAYILYPKTLGEGSFSDLDTFVFKLLPEVLEGQFG; YKLFIYGRDDYVGEDTIEVTNENVKKSRRLIIILVRDMGGFSWLGQSSEEQIAIYNALIQEGIKIVLLEL; EKIQDYEKMPDSIQFIKQKHGVICWSGDFQERPQSAKTRFWKNLRYQMPAQRRSPLSKHRLLTLDPVRDT; KEKLPAATHLPLG; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001123382
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001123382.1, NP_001116854.1
• RefSeq Size: 4808 bp
• RefSeq ORF: 1722 bp
• Locus ID: 16177
• Cytogenetics: 1 18.81 cM
• Summary: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).[UniProtKB/Swiss-Prot Function]