Il1r1 (NM_001123382) Mouse Untagged Clone

SKU MC219162

Il1r1 (untagged) - Mouse interleukin 1 receptor, type I (Il1r1), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Il1r1
• Synonyms: CD121a; CD121b; IL-1R1; IL-iR; Il1r-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219162 representing NM_001123382; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAAGTGCTACTGGGGCTCATTTGTCTCATGGTGCCTCTGCTGTCGCTGGAGATTGACGTATGTACAG; AATATCCAAATCAGATCGTTTTGTTTTTATCTGTAAATGAAATTGATATTCGCAAGTGTCCTCTTACTCC; AAATAAAATGCACGGCGACACCATAATTTGGTACAAGAATGACAGCAAGACCCCCATATCAGCGGACCGG; GACTCCAGGATTCATCAGCAGAATGAACATCTTTGGTTTGTACCTGCCAAGGTGGAGGACTCAGGATATT; ACTATTGTATAGTAAGAAACTCAACTTACTGCCTCAAAACTAAAGTAACCGTAACTGTGTTAGAGAATGA; CCCTGGCTTGTGTTACAGCACACAGGCCACCTTCCCACAGCGGCTCCACATTGCCGGGGATGGAAGTCTT; GTGTGCCCTTATGTGAGTTATTTTAAAGATGAAAATAATGAGTTACCCGAGGTCCAGTGGTATAAGAACT; GTAAACCTCTGCTTCTTGACAACGTGAGCTTCTTCGGAGTAAAAGATAAACTGTTGGTGAGGAATGTGGC; TGAAGAGCACAGAGGGGACTATATATGCCGTATGTCCTATACGTTCCGGGGGAAGCAATATCCGGTCACA; CGAGTAATACAATTTATCACAATAGATGAAAACAAGAGGGACAGACCTGTTATCCTGAGCCCTCGGAATG; AGACGATCGAAGCTGACCCAGGATCAATGATACAACTGATCTGCAACGTCACGGGCCAGTTCTCAGACCT; TGTCTACTGGAAGTGGAATGGATCAGAAATTGAATGGAATGATCCATTTCTAGCTGAAGACTATCAATTT; GTGGAACATCCTTCAACCAAAAGAAAATACACACTCATTACAACACTTAACATTTCAGAAGTTAAAAGCC; AGTTTTATCGCTATCCGTTTATCTGTGTTGTTAAGAACACAAATATTTTTGAGTCGGCGCATGTGCAGTT; AATATACCCAGTCCCTGACTTCAAGAATTACCTCATCGGGGGCTTTATCATCCTCACGGCTACAATTGTA; TGCTGTGTGTGCATCTATAAAGTCTTCAAGGTTGACATAGTGCTTTGGTACAGGGACTCCTGCTCTGGTT; TTCTTCCTTCAAAAGCTTCAGATGGAAAGACATACGATGCCTATATTCTTTATCCCAAGACCCTGGGAGA; GGGGTCCTTCTCAGACTTAGATACTTTTGTTTTTAAACTGTTGCCTGAGGTCTTGGAGGGACAGTTTGGA; TACAAGCTGTTCATTTATGGAAGGGATGACTATGTTGGAGAAGATACCATCGAGGTTACTAATGAAAATG; TAAAGAAAAGCAGGAGGCTGATTATCATTCTAGTGAGAGATATGGGAGGCTTCAGCTGGCTGGGCCAGTC; ATCTGAAGAGCAAATAGCCATATACAATGCTCTCATCCAGGAAGGAATTAAAATCGTCCTGCTTGAGTTG; GAGAAAATCCAAGACTATGAGAAAATGCCAGATTCTATTCAGTTCATTAAGCAGAAACACGGAGTCATTT; GCTGGTCAGGAGACTTTCAAGAAAGACCACAGTCTGCAAAGACCAGGTTCTGGAAAAACTTAAGATACCA; GATGCCAGCCCAACGGAGATCACCATTGTCTAAACACCGCTTACTAACCCTGGATCCTGTGCGGGACACT; AAGGAGAAACTGCCGGCAGCAACACACTTACCACTCGGCTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001123382
• Insert Size: 1722 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001123382.1, NP_001116854.1
• RefSeq Size: 4808 bp
• RefSeq ORF: 1722 bp
• Locus ID: 16177
• Cytogenetics: 1 18.81 cM
• Summary: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.