Enpp1 (NM_008813) Mouse Tagged ORF Clone

SKU MG227498

Enpp1 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Enpp1
• Synonyms: 4833416E15Rik; AI428932; C76301; CD203c; E-N; E-NPP 1; E-NPP1; Ly-4; Ly-41; M6S1; N; NPP1; Npps; P; PC-; PC-1; Pca; Pca-1; Pd; Pdnp1; ttw; twy
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227498 representing NM_008813; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCGCGACGGCGACCAGGCAGGGCACGGGCCCCGGCACGGGTCAGCGGGAAACGGTCGCGAGCTCG; AGTCACCAGCCGCGGCTTCGCTGCTCGCGCCCATGGACCTAGGAGAGGAGCCGCTGGAGAAGGCGGAGCG; TGCCCGCCCTGCCAAAGACCCCAACACCTACAAAGTGCTGTCGCTGGTTTTGTCAGTATGTGTGCTAACA; ACAATTCTTGGTTGTATATTTGGGTTGAAACCAAGCTGCGCCAAAGAAGTAAAAAGTTGCAAAGGCCGCT; GCTTTGAAAGGACGTTCAGCAACTGTCGCTGTGATGCTGCCTGTGTCAGCCTTGGGAACTGCTGTCTGGA; TTTCCAGGAGACCTGTGTGGAACCAACACATATATGGACTTGCAACAAATTCAGGTGCGGTGAGAAGAGG; CTGTCCAGGTTCGTGTGCTCCTGTGCAGACGACTGCAAAACCCACAATGACTGTTGCATCAACTACAGTT; CTGTGTGCCAAGATAAGAAGAGTTGGGTAGAAGAAACATGTGAAAGCATCGATACCCCGGAGTGTCCAGC; AGAGTTTGAATCACCCCCTACTCTCTTGTTTTCTTTGGATGGATTCAGAGCTGAGTATTTGCACACCTGG; GGTGGACTTCTTCCTGTCATTAGCAAGCTGAAAAACTGTGGAACATACACTAAAAACATGAGGCCTATGT; ACCCTACCAAGACGTTTCCCAATCATTACAGCATCGTCACAGGACTTTATCCGGAATCCCATGGCATAAT; TGATAACAAGATGTATGATCCCAAAATGAATGCTTCTTTCTCGCTTAAAAGTAAAGAGAAATTCAACCCT; TTGTGGTACAAAGGACAGCCGATTTGGGTGACCGCTAATCATCAGGAGGTCAAGTCCGGCACATACTTTT; GGCCAGGATCAGATGTGGAGATTGACGGGATTCTACCAGATATCTACAAAGTGTATAATGGGTCAGTACC; ATTTGAAGAAAGGATTTTAGCTGTTCTTGAGTGGCTGCAGCTTCCTAGCCATGAAAGACCACACTTTTAC; ACTCTGTATTTAGAAGAACCAGATTCTTCAGGGCATTCACATGGACCAGTCAGCAGCGAGGTCATCAAGG; CCTTGCAGAAGGTTGACCGCCTGGTCGGCATGCTGATGGACGGCCTGAAGGACCTGGGCTTGGATAAATG; CCTGAACCTCATCCTCATTTCAGATCACGGCATGGAACAAGGCAGCTGTAAGAAGTATGTGTACCTGAAT; AAGTACTTGGGGGATGTGAACAATGTGAAAGTTGTGTATGGACCTGCTGCTCGGTTGAGACCCACTGATG; TTCCAGAGACATACTATTCATTTAACTATGAAGCCCTTGCAAAAAATCTTTCTTGCCGGGAGCCAAACCA; GCATTTCCGGCCTTACCTGAAACCCTTCTTACCCAAGCGCTTACACTTCGCTAAAAGTGACCGGATTGAG; CCCCTGACCTTCTACCTGGACCCTCAGTGGCAACTTGCGTTGAATCCATCAGAGAGGAAATATTGTGGAA; GTGGATTTCATGGCTCTGACAACTTGTTTTCAAACATGCAAGCTCTTTTTATTGGCTATGGACCTGCCTT; CAAGCATGGTGCTGAAGTTGACTCCTTTGAAAATATTGAAGTCTATAACTTAATGTGTGACTTACTGGGT; TTGATCCCAGCTCCCAATAATGGAAGTCATGGCAGCCTTAACCACCTTCTAAAGAAACCCATTTACAACC; CAAGTCATCCCAAAGAAGAGGGCTTCCTGTCTCAGTGTCCAATCAAATCAACATCCAATGACCTCGGCTG; CACATGTGACCCTTGGATTGTGCCAATCAAGGACTTTGAGAAACAGCTTAATCTGACCACAGAAGATGAT; GACATTTACCATATGACTGTACCCTACGGGAGGCCCCGGATTCTACTGAAGCAGCACCGCGTCTGTCTAC; TCCAGCAGCAACAGTTCTTGACCGGATACAGCCTGGACCTCCTCATGCCCCTCTGGGCGTCTTACACCTT; CCTCAGTAATGACCAATTCTCCAGAGATGACTTTTCCAACTGTCTGTACCAGGATCTTCGGATTCCCCTT; AGTCCTGTGCATAAATGTTCCTATTACAAAAGCAATTCTAAGCTAAGTTATGGGTTCCTCACGCCACCGA; GACTAAATAGAGTTTCAAATCACATATACTCTGAAGCGCTGCTTACATCTAATATAGTGCCAATGTATCA; GAGTTTTCAAGTTATATGGCACTACCTTCATGACACCCTACTGCAAAGGTATGCCCATGAAAGGAACGGC; ATCAATGTTGTCAGCGGTCCCGTGTTTGACTTTGATTATGACGGACGCTATGATTCCTTAGAGATCCTGA; AACAAAACAGCAGAGTCATCCGAAGCCAGGAAATTCTGATTCCGACTCACTTCTTCATTGTGCTCACCAG; CTGCAAGCAGCTGTCTGAGACTCCCTTGGAGTGCTCCGCCTTAGAGTCCTCAGCTTACATACTGCCTCAT; AGGCCTGATAACATTGAGAGCTGTACGCATGGGAAGCGGGAGTCTTCATGGGTCGAGGAGTTGCTGACCT; TGCACAGAGCTCGGGTCACAGACGTGGAACTCATCACTGGCCTCAGCTTCTACCAGGACCGACAAGAGTC; AGTTTCAGAACTGCTGAGGTTGAAAACACATTTGCCAATCTTCAGCCAAGAAGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227498 representing NM_008813; Red=Cloning site Green=Tags(s); ; MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVLSLVLSVCVLT; TILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKR; LSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTW; GGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNP; LWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFY; TLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLN; KYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIE; PLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLG; LIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDD; DIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPL; SPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNG; INVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPH; RPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008813
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008813.4, NP_032839.3
• RefSeq Size: 3224 bp
• RefSeq ORF: 2718 bp
• Locus ID: 18605
• Cytogenetics: 10 12.26 cM
• Summary: This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]