Magel2 (NM_013779) Mouse Tagged ORF Clone

SKU MG224465

Magel2 (tGFP-tagged) - Mouse melanoma antigen family L 2 (Magel2), (10ug). Note: ORF is codon optimized

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Magel2
• Synonyms: Mage-l2; NDNL1; nM15; ns7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224465 representing NM_013779, codon optimized.; Due to the complexity of NM_013779, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCCCAGCTTTCCACCAACCTGGGTGATTCATCACCTCCGGAATCACCAGTGCCCGCAGTCCACTCCA; GGCCCACAGTACTGATGCGGGCTCCTCCCGCTAGTTCAAGGGCTCCTCCTGTCCCGTGGGATCCTCCCCC; CGTTGATCTCCAGGCCCCTATGGCTGCATGGCAGGCCCCCCAGCCAGCATGGGAAGCTCCGGAGGGTCAG; CTGCCTGCGCCTGTCGCCCAGCTGGCTCAGCCTCCAGGGCTGGGCGCACCTATGGTTCAGGCGCCCCCTC; TGGGTGGCGGCATGGCTAAACCACCTACTCCAGGAGTCCTGATGGTGCACCAGCCACCCCCTGGCGCGCC; TATGGCTCAGTCAAGCACGCCCGGCGTGCTGATGCTGCACCCTTCTGTGACTGGCGCCCCCCTGGCTCAC; CCCCCCCCACCCGGTACACCCATGACACACCCACCCGGGACATCCATGGCCCATCCCCCTCCTCCTCCGC; CACCTCCGCCTCCCCCCCCACCTCCGGGCACACCCATGACTCACCCACCTCCTCCGGGAACACCTATGGG; ACACCACCCCCCACCGGGAAATCCGATGACCCACCCCCCTCCTGGCAATCCTATGGTTCACCCCCTCACC; CATGGCGCGCCTATGGTGCATGGAGGTCCCCATGGGACTCCTATGCCACATGTGCCCATCACGGGGACCC; CTATTGCTCAGCAACCGACACCCGGTGTCCTGATGGCGCAGCAGCTTACTCCCGGAGTGCTCATGGTGCA; GCCTCCTGCTCCTGGAGCACCAATGGTGCAGCCCCCCCCTCAGGCTGCCCTCATGACCCAACCAGCCCCA; AGTATAACACCAATGGCAAAGCCTCCTGGACCCGGCGTGGTAATGATACATCCTCCAGGCGCTCGAGGCC; CCATTATTCAGACACCCGTATCCGGCGCTCCTATGGCTCAAACCGTGCTCCCCCCGGGTCAGCCTTTGGC; CACTTGGGCACCACAGGGCCAGCCCCTCATCCTGCAGATCCAAAGCCAGGTCATCCGCGCCCCCCCTCAG; GTGCCCAGCGTTCCCCAGGCACCTCAGGTTCAGCTTGCCACACCTCCTGGATGGCAGGCGACAACGCCAA; ATTGGCAGGTCACCCCCCAGGGATGGCCGGCTACTCCTCTGACGTGGCAGGCAACACAAGTGACCTGGCA; GGCGCCAACCATTGCATGGCAGGCCACACAGCCAGGCAGGCAGGGGCACTCCACAATCAGGACAGGGCAT; ACACCCATCCGCCCAGGCCCCGCACCGCTCCTGCGGCAGATCCCGCCTATGATCAGGCAAATTCAGCCCG; TCATGAGGCAGGCCCCCCCACTTATTCGCCAGGTGCCAATCCGCCCCGCGCCACATGGGATCGCCAGCCA; GCCTCAGCTGTGGCAGGTGCTCCCTCCACCACCACCTCTGCGGCAGGCCCCCCAGGCACGGCTTCTCCTT; CCACGAGTGCCCGGTACGGGGCAGGTCAGCACGGTCCCTCCTGTTGCCCAGATTCACCTGGTGCCCCAAA; GCGGACCACAGGTTCCCCAAACAGTGCTGCCAGCCCAGCTTAGTATTCCCATCCCTGTACCCCAAGCAGC; CGCCCAGTCCGCCCCCCGCACTGTGCATTGCCCGCCGATCATTTGGCAAGCTCCCAAAGGTCAGGCTCCC; GTCCCTCAGGAACTTCCCGTGCCACAAGAGCTGCCAGTACCCCAGGAGCTGCCCGTTCCCCAGGAGGTGC; CAGTTCCACAGGAGATCCCCGTGCCTCAGGAGATCCCAGTTCCTCAGGAACTGCCAGTACCCCAAGAACT; TCCGGTGCCTCAGGAGCTGCCGGTGCCTCAAGAGCTGCCTGTCCCACAGGAACTTCCTGTCCCTCAGGAG; CTGCCAGTGCCACAGGAGCTGCCCGTACCACAGGAGCTGCCAGTCCCACTGGAGTTCCAGGAGGTCCAAC; AGGCTCAGGCAGTCGGATGGCGCGCTCCTAAAGTCCCACCCCACTTTTGGCAGCCTGTGAGCGCACAAGA; GGCCCAGGAACAAGCAACCCAGATTGCGCACGTGGAACAACAGCAGCCTTTCCAGGGAGCCCCCGCATCC; TCTAAAGCGCTGCAAACCCAACTCCCAACTCACCAAGCCCAGGCCTCCGGCCTCCAGGCGGAGCTGCCTT; CCGTGCAGCTGCAACCCAGCTGGCAGGGTCCTCTGCCCATGTTGCAGGCCCAGCCCGGGGCTTCAGCAAC; CCTTGCTAACTTTCCTAGGGGCTCCACCAGAAGCAGAATGGCCCCAAGCGGTGAACCGGGTCCATCTTCC; CTTGAGCCGCGGGGACCCCCACGAGAACGGCGAGCACCCGCCCGCGACAAAAAGGGCCCTCCGAAAGAAA; GGATGTTCATCGGAGCGACCTTTTGTGCCCCCAGGGGCGCAAGCGCGTCCCGCGCATACGTGCCAACGGC; ATGGAAGAATCTCCCTGCTACCAGCGAAACCTTCCCGGCCACAAGCAGGGTATTCCCTTCCACAAGTCAC; TTTCAGCCCGCCTCATCTAATGCCTTTCGCGGTCCTAGCGCCGCTAGTGAGTCTCCTAAAAGCTTGCCCT; TTGCACTGCAGGATCCCTACGCCTGTGTGGAAGCCCTCCCGGCGGTGCCGTGGGTCCCTTATCCCGACGG; CAACGCTAGTTCTGCATGCAAATCCGTCCCAGCAATACTGATGGTGGCCGCTGCTGCCCCGCAGGCATCT; GCTACGGCCGCCGAAGCCTCTAAGAGCTCTGAGCCCCCTCGGCGGCCCGGCAAGGCTACAAGGAAGAAGA; AGCATCTGGAGCCAAAGGAGGACAACTGTGGACATAGGCTGAGTAGCAGAGACTGGAGAGGTCCCCGCAC; ATGGGGCAATCCTAGTCACAGCGACTGGGAAATCCAACGCGCAATGCAACTGCTCGGGGACCGCGAGTCA; TTGTATACCCCTCAGGGCCTCAACGATTGGGGCTGTCCAAATACCAGCCGCATGCCAAGATCCTTGGAAG; GACCCAGCACCTCCCGGGATCAGGAGTTCTGCGGCGATTCTGGGGGGAGTCAAACATGGATGGCTTCTGA; AGTTCCTAGCGTATCCAGAGGCTCCTCTGCGGCACAAGAAGATCCTGACCGCGAGAGCCAGCCACTGAGT; CCCCTGGATGAAAGAGCTAATGCTCTCGTGCAATTTCTGTTGGTTAAGGACCAGGCTAAGGTTCCGGTGC; AGCTCAGTGAGATGGTCAATGTGGTCATCCGGGAGTACAAGGACGACTCACTGGACATCATCAACCGCGC; CAATACGAAACTCGAATGCACATTCGGGTGTCAACTCAAGGAGGTTGATACCAAAACGCACACTTACATC; ATAGTTAATAAGATGGCCTACCCACAGTGCAACCTGTTGGCTTCCTATCTGGAACGCCCTAAATTTAGCC; TCCTGATGGTGGTCCTGTCACTCATTTTCATGAAGGGATATTGTATTCGGGAGAATCTGCTCTTCAGCTT; TCTGTTCCAGCTGGGTCTGGATGTACAAGAGACAAGCGGACTCTTTCGAATTACAAAGAAACTGATTACT; AGCGTCTTTGTACGGCATCGATACCTGGAGTACCGACAGATCCCCTTTACTGAGCCTGCTGAGTACGAGC; TTCTCTGGGGCCCCCGAGCGTTTTTGGAGACTAACAGAGTGCACATTCTGAGATTTTTGGCGGCGCTTTA; CGAAAATCAACCTCAAATATGGTCATGTCAGTACCTGGACAGCCTCGCCGAGTTGGAATACAAGGACGCG; AACGCCGCAGCTGAGGAGAGCCATGATTCAGACGACGATGCACACGACCCCACATCCTCCCCTCACCCCC; AC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224465 representing NM_013779; Red=Cloning site Green=Tags(s); ; MSQLSTNLGDSSPPESPVPAVHSRPTVLMRAPPASSRAPPVPWDPPPVDLQAPMAAWQAPQPAWEAPEGQ; LPAPVAQLAQPPGLGAPMVQAPPLGGGMAKPPTPGVLMVHQPPPGAPMAQSSTPGVLMLHPSVTGAPLAH; PPPPGTPMTHPPGTSMAHPPPPPPPPPPPPPPGTPMTHPPPPGTPMGHHPPPGNPMTHPPPGNPMVHPLT; HGAPMVHGGPHGTPMPHVPITGTPIAQQPTPGVLMAQQLTPGVLMVQPPAPGAPMVQPPPQAALMTQPAP; SITPMAKPPGPGVVMIHPPGARGPIIQTPVSGAPMAQTVLPPGQPLATWAPQGQPLILQIQSQVIRAPPQ; VPSVPQAPQVQLATPPGWQATTPNWQVTPQGWPATPLTWQATQVTWQAPTIAWQATQPGRQGHSTIRTGH; TPIRPGPAPLLRQIPPMIRQIQPVMRQAPPLIRQVPIRPAPHGIASQPQLWQVLPPPPPLRQAPQARLLL; PRVPGTGQVSTVPPVAQIHLVPQSGPQVPQTVLPAQLSIPIPVPQAAAQSAPRTVHCPPIIWQAPKGQAP; VPQELPVPQELPVPQELPVPQEVPVPQEIPVPQEIPVPQELPVPQELPVPQELPVPQELPVPQELPVPQE; LPVPQELPVPQELPVPLEFQEVQQAQAVGWRAPKVPPHFWQPVSAQEAQEQATQIAHVEQQQPFQGAPAS; SKALQTQLPTHQAQASGLQAELPSVQLQPSWQGPLPMLQAQPGASATLANFPRGSTRSRMAPSGEPGPSS; LEPRGPPRERRAPARDKKGPPKERMFIGATFCAPRGASASRAYVPTAWKNLPATSETFPATSRVFPSTSH; FQPASSNAFRGPSAASESPKSLPFALQDPYACVEALPAVPWVPYPDGNASSACKSVPAILMVAAAAPQAS; ATAAEASKSSEPPRRPGKATRKKKHLEPKEDNCGHRLSSRDWRGPRTWGNPSHSDWEIQRAMQLLGDRES; LYTPQGLNDWGCPNTSRMPRSLEGPSTSRDQEFCGDSGGSQTWMASEVPSVSRGSSAAQEDPDRESQPLS; PLDERANALVQFLLVKDQAKVPVQLSEMVNVVIREYKDDSLDIINRANTKLECTFGCQLKEVDTKTHTYI; IVNKMAYPQCNLLASYLERPKFSLLMVVLSLIFMKGYCIRENLLFSFLFQLGLDVQETSGLFRITKKLIT; SVFVRHRYLEYRQIPFTEPAEYELLWGPRAFLETNRVHILRFLAALYENQPQIWSCQYLDSLAELEYKDA; NAAAEESHDSDDDAHDPTSSPHPH; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013779
• ORF Size: 3852 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013779.2, NP_038807.4
• RefSeq Size: 4662 bp
• RefSeq ORF: 3855 bp
• Locus ID: 27385
• UniProt ID: Q9QZ04
• Cytogenetics: 7 34.37 cM
• Summary: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (PubMed:22208286).[UniProtKB/Swiss-Prot Function]