Magel2 (NM_013779) Mouse Untagged Clone

SKU MC224146

Magel2 (untagged) - Mouse melanoma antigen, family L, 2 (Magel2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Magel2
• Synonyms: Mage-l2; NDNL1; nM15; ns7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224146 representing NM_013779; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGCAGCTAAGTACGAATCTGGGGGATTCCAGCCCTCCGGAGTCCCCAGTGCCTGCAGTCCATAGCC; GCCCTACGGTTCTGATGCGGGCTCCGCCTGCTTCCTCCCGGGCTCCTCCTGTCCCCTGGGATCCACCTCC; AGTGGACTTGCAGGCTCCCATGGCTGCTTGGCAGGCTCCTCAACCTGCCTGGGAGGCTCCGGAGGGCCAG; CTGCCTGCCCCAGTGGCTCAGCTGGCCCAGCCTCCTGGTCTAGGGGCCCCAATGGTCCAGGCTCCACCGC; TCGGAGGGGGGATGGCCAAGCCTCCAACTCCTGGAGTCTTGATGGTCCATCAGCCCCCTCCGGGAGCCCC; CATGGCCCAGTCTTCAACTCCGGGAGTCCTGATGCTACATCCTTCTGTCACGGGGGCCCCTTTGGCTCAT; CCTCCTCCCCCAGGAACCCCGATGACACACCCTCCCGGGACCTCGATGGCGCACCCTCCTCCTCCTCCTC; CTCCCCCTCCTCCTCCTCCTCCTCCTGGGACCCCAATGACCCACCCACCTCCTCCTGGGACTCCGATGGG; TCACCATCCTCCTCCTGGGAACCCTATGACCCATCCTCCACCGGGGAACCCGATGGTGCATCCTCTCACT; CATGGAGCCCCGATGGTCCATGGGGGACCACATGGAACTCCAATGCCGCATGTTCCCATTACGGGGACAC; CGATAGCCCAGCAGCCAACTCCAGGAGTCCTGATGGCCCAGCAGCTGACACCGGGAGTCCTGATGGTCCA; GCCGCCTGCTCCGGGAGCTCCGATGGTCCAGCCACCTCCACAGGCTGCCTTGATGACCCAGCCTGCACCT; TCGATTACTCCGATGGCCAAGCCTCCAGGTCCTGGTGTCGTGATGATCCATCCTCCAGGTGCCAGAGGTC; CAATCATTCAGACTCCAGTATCAGGAGCACCAATGGCTCAGACAGTGCTGCCCCCTGGGCAGCCTCTGGC; CACTTGGGCCCCACAGGGTCAGCCTTTGATCCTACAAATCCAGTCTCAAGTCATAAGGGCTCCTCCACAG; GTTCCCTCTGTACCACAAGCCCCCCAGGTACAGCTGGCCACACCCCCAGGCTGGCAAGCCACCACACCCA; ACTGGCAGGTGACCCCCCAGGGGTGGCCAGCAACGCCTTTGACATGGCAGGCTACACAGGTGACCTGGCA; GGCCCCCACAATAGCCTGGCAGGCCACTCAACCTGGGCGCCAAGGGCACTCAACCATTCGTACTGGTCAC; ACACCCATTCGACCTGGACCAGCTCCATTGCTTCGCCAGATACCTCCTATGATCCGTCAGATCCAACCTG; TGATGAGGCAAGCCCCACCACTGATCCGACAGGTCCCTATCAGACCAGCTCCACATGGCATAGCAAGCCA; GCCTCAGCTGTGGCAGGTCCTGCCACCCCCACCTCCACTGCGGCAGGCTCCACAGGCTCGTCTACTGCTC; CCGAGGGTACCAGGAACAGGCCAGGTGTCTACAGTACCACCAGTTGCTCAGATACATTTGGTGCCACAGT; CAGGCCCACAGGTGCCCCAGACAGTACTGCCAGCCCAACTGTCTATCCCAATTCCTGTACCCCAGGCTGC; TGCTCAGTCTGCTCCCCGGACTGTGCATTGCCCACCCATCATCTGGCAGGCCCCCAAAGGCCAGGCCCCG; GTGCCACAGGAGCTCCCAGTGCCACAGGAGCTCCCGGTGCCACAGGAGCTCCCGGTGCCACAAGAGGTCC; CGGTTCCACAGGAGATCCCGGTGCCACAGGAGATCCCGGTGCCACAGGAGCTCCCGGTTCCACAGGAGCT; CCCGGTGCCACAGGAGCTCCCAGTGCCACAAGAGCTCCCGGTGCCACAGGAGCTCCCAGTGCCACAGGAG; CTCCCGGTGCCACAGGAGCTCCCGGTGCCACAGGAGCTCCCGGTGCCATTGGAGTTCCAGGAGGTACAGC; AGGCCCAGGCAGTGGGCTGGCGGGCACCCAAGGTACCTCCTCACTTCTGGCAGCCTGTGTCTGCCCAGGA; GGCCCAGGAGCAGGCCACTCAGATCGCCCATGTGGAGCAGCAGCAGCCCTTTCAGGGAGCTCCAGCCTCC; TCCAAGGCACTGCAAACTCAGCTGCCGACCCACCAGGCCCAAGCCTCTGGCTTGCAGGCAGAACTGCCTT; CAGTGCAGCTGCAGCCTTCTTGGCAAGGCCCACTGCCCATGTTGCAGGCCCAGCCTGGAGCCTCTGCCAC; ACTGGCAAACTTTCCCCGGGGCTCCACTCGATCACGTATGGCTCCATCAGGAGAACCTGGCCCCTCTTCT; CTAGAACCTCGGGGCCCTCCTAGGGAACGTAGGGCACCTGCAAGGGACAAAAAGGGTCCTCCAAAAGAGC; GCATGTTCATTGGTGCCACTTTCTGTGCTCCAAGGGGGGCATCAGCATCCAGGGCATACGTGCCAACTGC; CTGGAAAAACTTGCCTGCCACATCAGAGACCTTTCCTGCCACCTCAAGGGTCTTTCCATCTACCTCTCAT; TTCCAGCCTGCCTCTTCTAATGCCTTTAGAGGTCCATCTGCCGCCTCAGAGAGCCCAAAGTCACTGCCAT; TTGCTCTGCAGGATCCTTATGCCTGCGTAGAGGCCCTGCCTGCAGTTCCCTGGGTTCCGTATCCAGATGG; AAATGCCTCATCAGCATGTAAGTCAGTGCCTGCCATCTTGATGGTGGCAGCAGCTGCCCCCCAGGCAAGT; GCCACTGCTGCAGAGGCCTCTAAGTCTTCAGAGCCGCCAAGACGCCCCGGCAAAGCCACCAGGAAGAAGA; AGCATCTGGAACCCAAAGAAGACAACTGTGGCCACAGGCTCTCCTCACGTGACTGGCGGGGGCCCCGAAC; CTGGGGCAATCCCAGTCATTCTGACTGGGAGATTCAGAGGGCTATGCAGCTCCTGGGGGACCGGGAATCC; CTCTACACTCCGCAGGGCCTGAATGACTGGGGGTGCCCCAACACTTCTAGGATGCCAAGGAGCTTGGAGG; GCCCTAGCACTTCACGGGACCAGGAATTCTGTGGTGACTCGGGTGGGTCTCAGACATGGATGGCTTCTGA; GGTCCCAAGCGTCTCTCGGGGATCCAGTGCTGCTCAGGAGGACCCTGATAGGGAGAGTCAGCCCTTATCT; CCCTTAGATGAGAGAGCAAACGCTTTGGTGCAGTTTCTCTTGGTCAAAGACCAAGCCAAGGTGCCTGTCC; AGCTCTCGGAGATGGTAAATGTTGTCATCCGAGAATACAAAGACGACAGCTTAGACATCATCAACCGTGC; CAACACTAAGCTGGAGTGCACCTTTGGTTGTCAACTGAAGGAAGTTGACACCAAAACCCACACTTACATC; ATCGTCAACAAGATGGCGTACCCTCAGTGTAATTTGCTGGCATCCTATTTAGAGAGGCCAAAGTTCAGCC; TCCTGATGGTGGTCTTGAGCCTCATCTTTATGAAAGGCTACTGTATCAGGGAGAATCTGCTCTTTAGTTT; TCTGTTCCAGCTAGGGCTGGATGTCCAGGAGACAAGTGGTCTCTTCAGAATTACAAAGAAGCTCATCACC; AGTGTGTTTGTGAGACACAGGTACCTAGAGTACAGGCAAATCCCGTTCACTGAGCCTGCAGAATACGAGC; TTCTCTGGGGGCCCCGGGCATTCCTCGAAACCAACAGGGTGCACATCTTGAGATTTTTGGCCGCACTCTA; CGAGAACCAGCCCCAGATCTGGTCATGCCAGTACCTTGACTCACTGGCAGAGTTAGAATACAAGGACGCA; AATGCCGCCGCCGAAGAGTCCCATGACAGCGATGATGATGCCCACGACCCCACCAGCAGTCCCCATCCTC; ACTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013779
• Insert Size: 3855 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013779.2, NP_038807.4
• RefSeq Size: 4662 bp
• RefSeq ORF: 3855 bp
• Locus ID: 27385
• UniProt ID: Q9QZ04
• Cytogenetics: 7 34.37 cM
• Summary: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (PubMed:22208286).[UniProtKB/Swiss-Prot Function]