Dhx58 (NM_030150) Mouse Tagged ORF Clone

SKU MG209973

Dhx58 (tGFP-tagged) - Mouse DNA segment, Chr 11, Lothar Hennighausen 2, expressed (D11Lgp2e)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Dhx58
• Synonyms: B430001I08Rik; D11Lgp2e; Lgp2; LPG2; RLR-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209973 representing NM_030150; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCTGCGACCCTACCAGTGGGAAGTGATCTTACCTGCTCTGGAGGGCAAGAATATCATTATATGGC; TGCCCACGGGTGCTGGGAAGACCCGGGCAGCTGCCTTTGTAGCCAAGAGGCATCTAGAGACGGTAGACAG; AGGCAAGGTGGTGGTACTGGTCAATAGGGTACACCTGGTGAGCCAGCACGCCGAGGAGTTCAGGCGCATG; CTGGATAAACACTGGACCGTAACAACCCTGAGTGGGGACATGGGATCCCGAGCTGGCTTTGGCCTGATGG; CTCGGAGCCACGACCTGCTCATCTGTACGGCAGAGTTGTTACAGTTGGCACTCAACAGCTCTGAGGAGGA; TGAACACGTAGAGCTCAGAGAATTCTCGCTGATTGTGGTGGACGAGTGTCACCACACCCACAAGGACACC; GTCTACAACACCATCTTGAGCCGGTACCTAGAACAGAAGCTGAAGAAGGCAGAGCCCCTCCCCCAGGTCC; TGGGTCTCACAGCCTCCCCAGGCACTGGAGGGGCCACCAAGCTCCAAGGGGCCATTGATCACATCCTACA; GCTTTGTGCGAATTTAGATACGTGCCACATCATGTCGCCAAAGAATTGCTACTCCCAGCTGCTGATGCAT; AACCCGAAGCCCTGCAAGCAGTATGACCTCTGCCAAAGGCGCGCACAGGATCCTTTTGGGGACTTGATAA; AAAAGCTTATGAACCAAATCCACCAACAACTAGAGATGCCTGACTTGAAGCAACAATTTGGAACCCAGAT; GTATGAGCAGCAAGTGGTACAGTTGTGCAAGGATGCGGCAGAGGCTGGACTCCAGGAACAGCGGGTGTAT; GCGCTGCATTTGCGGCGCTACAATGATGCGCTATTTATCCACGATACTGTCCGTGCCCGGGACGCCCTTG; ACATGTTGCAAGATTTTTACGACAGAGAACGCACCACAAAAACACAGATGGTGCGTGCTGAAAGCTGGCT; GCTGAAGCTGTTTGATGACCATAAAAATGTGCTGGGCCAGCTAGCAGCTCGGGGTCCTGAGAACCCGAAG; TTGGAGATGCTGGAAAGGATCTTACTGAAGCAGTTTGGGAGTCCTGGCCACACTCGGGGTATCATCTTCA; CCAGAACCCGTCAGACTGCTTCCTCCCTCCTGCTCTGGCTTCGGCAGCAGCCTTGCCTACAGACTGTGGG; CATCAAGCCACAGATGCTGATCGGAGCAGGGAACACAAGCCAGAGCACACACATGACCCAGAAAGACCAG; CAGGAAGTGATCCAGGAGTTCAGGGATGGTATCCTGAGCCTTCTAGTGGCCACAAGTGTGGCAGAGGAGG; GGCTGGATATCGCTCAGTGCAATGTGGTGGTGCGCTATGGGCTCCTGACCAATGAGATCTCCATGGTCCA; GGCCCGGGGTCGTGCTCGAGCTGGTCAGAGTGTGTACTCCTTCCTGGCTACAGAGGGCAGTCGGGAGATG; AAGCGGGAGCTAACCAATGAGGCTCTGGAGGTGCTGATGGAGAAGGCTGTGGCTGCTGTACAGAAGATGG; ACCCTGATGAGTTCAAGGCCAAGATCCGGGACTTGCAGCAAGCATCTCTCGTTAAGCGGGCAGCACGCGC; GGCCCATCGGGAGATCCAGCAGGGGCAGTTCCTACCGGAGCACGTGCAACTTCTCTGCATCAACTGTATG; GTGGCCGTGGGCTACGGGAGTGACCTGCGGAAAGTGGAGGGCACCCACCACGTCAATGTGAACCCCAACT; TCTCGGTCTACTATACCACCTCCCAGAACCCTGTGGTCATTAACAAGGTCTTTAAGGACTGGAGACCTGG; AGGAACCATCAGGTGCAGTAACTGTGGGGAGGTCTGGGGCTTCCAGATGATCTACAAATCAGTGACCTTG; CCAGTGCTCAAAATCGGAAGCATGCTACTGGAAACACCTCGAGGGAAGATCCAGGCCAAAAAGTGGTCCC; GGGTGCCTTTCTCCATACCAGTCTTCGATATCCTGCAGGACTGCACACAAAGCCTGTCTGAGCTCTCCCT; GGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209973 representing NM_030150; Red=Cloning site Green=Tags(s); ; MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVSQHAEEFRRM; LDKHWTVTTLSGDMGSRAGFGLMARSHDLLICTAELLQLALNSSEEDEHVELREFSLIVVDECHHTHKDT; VYNTILSRYLEQKLKKAEPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTCHIMSPKNCYSQLLMH; NPKPCKQYDLCQRRAQDPFGDLIKKLMNQIHQQLEMPDLKQQFGTQMYEQQVVQLCKDAAEAGLQEQRVY; ALHLRRYNDALFIHDTVRARDALDMLQDFYDRERTTKTQMVRAESWLLKLFDDHKNVLGQLAARGPENPK; LEMLERILLKQFGSPGHTRGIIFTRTRQTASSLLLWLRQQPCLQTVGIKPQMLIGAGNTSQSTHMTQKDQ; QEVIQEFRDGILSLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFLATEGSREM; KRELTNEALEVLMEKAVAAVQKMDPDEFKAKIRDLQQASLVKRAARAAHREIQQGQFLPEHVQLLCINCM; VAVGYGSDLRKVEGTHHVNVNPNFSVYYTTSQNPVVINKVFKDWRPGGTIRCSNCGEVWGFQMIYKSVTL; PVLKIGSMLLETPRGKIQAKKWSRVPFSIPVFDILQDCTQSLSELSLD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030150
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030150.2, NP_084426.2
• RefSeq Size: 2427 bp
• RefSeq ORF: 2037 bp
• Locus ID: 80861
• UniProt ID: Q99J87
• Cytogenetics: 11 63.52 cM
• Summary: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.[UniProtKB/Swiss-Prot Function]