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Dhx58 (NM_030150) Mouse Untagged Clone

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SKU
MC205827
Dhx58 (untagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $622.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dhx58
Synonyms B430001I08Rik; D11Lgp2e; Lgp2; LPG2; RLR-3
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC029209
GCAGGCTCCAGAATGGAGCTGCGACCCTACCAGTGGGAAGTGATCTTACCTGCTCTGGAGGGCAAGAATA TCATTATATGGCTGCCCACGGGTGCTGGGAAGACCCGGGCAGCTGCCTTTGTAGCCAAGAGGCATCTAGA GACGGTAGACAGAGGCAAGGTGGTGGTACTGGTCAATAGGGTACACCTGGTGAGCCAGCACGCCGAGGAG TTCAGGCGCATGCTGGATAAACACTGGACCGTAACAACCCTGAGTGGGGACATGGGATCCCGAGCTGGCT TTGGCCTGATGGCTCGGAGCCACGACCTGCTCATCTGTACGGCAGAGTTGTTACAGTTGGCACTCAACAG CTCTGAGGAGGATGAACACGTAGAGCTCAGAGAATTCTCGCTGATTGTGGTGGACGAGTGTCACCACACC CACAAGGACACCGTCTACAACACCATCTTGAGCCGGTACCTAGAACAGAAGCTGAAGAAGGCAGAGCCCC TCCCCCAGGTCCTGGGTCTCACAGCCTCCCCAGGCACTGGAGGGGCCACCAAGCTCCAAGGGGCCATTGA TCACATCCTACAGCTTTGTGCGAATTTAGATACGTGCCACATCATGTCGCCAAAGAATTGCTACTCCCAG CTGCTGATGCATAACCCGAAGCCCTGCAAGCAGTATGACCTCTGCCAAAGGCGCGCACAGGATCCTTTTG GGGACTTGATAAAAAAGCTTATGAACCAAATCCACCAACAACTAGAGATGCCTGACTTGAAGCAACAATT TGGAACCCAGATGTATGAGCAGCAAGTGGTACAGTTGTGCAAGGATGCGGCAGAGGCTGGACTCCAGGAA CAGCGGGTGTATGCGCTGCATTTGCGGCGCTACAATGATGCGCTATTTATCCACGATACTGTCCGTGCCC GGGACGCCCTTGACATGTTGCAAGATTTTTACGACAGAGAACGCACCACAAAAACACAGATGGTGCGTGC TGAAAGCTGGCTGCTGAAGCTGTTTGATGACCATAAAAATGTGCTGGGCCAGCTAGCAGCTCGGGGTCCT GAGAACCCGAAGTTGGAGATGCTGGAAAGGATCTTACTGAAGCAGTTTGGGAGTCCTGGCCACACTCGGG GTATCATCTTCACCAGAACCCGTCAGACTGCTTCCTCCCTCCTGCTCTGGCTTCGGCAGCAGCCTTGCCT ACAGACTGTGGGCATCAAGCCACAGATGCTGATCGGAGCAGGGAACACAAGCCAGAGCACACACATGACC CAGAAAGACCAGCAGGAAGTGATCCAGGAGTTCAGGGATGGTATCCTGAGCCTTCTAGTGGCCACAAGTG TGGCAGAGGAGGGGCTGGATATCGCTCAGTGCAATGTGGTGGTGCGCTATGGGCTCCTGACCAATGAGAT CTCCATGGTCCAGGCCCGGGGTCGTGCTCGAGCTGGTCAGAGTGTGTACTCCTTCCTGGCTACAGAGGGC AGTCGGGAGATGAAGCGGGAGCTAACCAATGAGGCTCTGGAGGTGCTGATGGAGAAGGCTGTGGCTGCTG TACAGAAGATGGACCCTGATGAGTTCAAGGCCAAGATCCGGGACTTGCAGCAAGCATCTCTCGTTAAGCG GGCAGCACGCGCGGCCCATCGGGAGATCCAGCAGGGGCAGTTCCTACCGGAGCACGTGCAACTTCTCTGC ATCAACTGTATGGTGGCCGTGGGCTACGGGAGTGACCTGCGGAAAGTGGAGGGCACCCACCACGTCAATG TGAACCCCAACTTCTCGGTCTACTATACCACCTCCCAGAACCCTGTGGTCATTAACAAGGTCTTTAAGGA CTGGAGACCTGGAGGAACCATCAGGTGCAGTAACTGTGGGGAGGTCTGGGGCTTCCAGATGATCTACAAA TCAGTGACCTTGCCAGTGCTCAAAATCGGAAGCATGCTACTGGAAACACCTCGAGGGAAGATCCAGGCCA AAAAGTGGTCCCGGGTGCCTTTCTCCATACCAGTCTTCGATATCCTGCAGGACTGCACACAAAGCCTGTC TGAGCTCTCCCTGGACTGACCCCCTTGTGACTGCAGTACCTGGGTCAAACTGCAGGGGGCAGGAGAGTCC AGCAAGCCATCCTCCTTCCCCTGGCTAGCACTCTGGTCAGGGTACCCCTTTATTCTGCTGCAGGGATCTT AAACACATTGGATGGAGTTGGAAGATGAACGCAGGAGAATTATTATGCCATGATGTTCTAAGAACTACAA CATCCCTCCCTTGCCCAAGCACCTTGTAGGTTTTGTTTTTGTTTTTGAGGCTCTTCATAAATTAAAGGTG TAGTAGAGAATAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_030150
Insert Size 2037 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC029209, AAH29209
RefSeq Size 2336 bp
RefSeq ORF 2037 bp
Locus ID 80861
UniProt ID Q99J87
Cytogenetics 11 63.52 cM
Summary Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.UniProtKB/Swiss-Prot Function
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"NM_030150" in other vectors (4)
SKU Description Size Price
MG209973 Dhx58 (tGFP-tagged) - Mouse DNA segment, Chr 11, Lothar Hennighausen 2, expressed (D11Lgp2e) 10 ug
$822.00
MR209973 Dhx58 (Myc-DDK-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58) 10 ug
$589.00 $622.00
MR209973L3 Lenti ORF clone of Dhx58 (Myc-DDK-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58) 10 ug
$922.00
MR209973L4 Lenti ORF clone of Dhx58 (mGFP-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58) 10 ug
$922.00

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