Cldn9 (NM_020293) Mouse Tagged ORF Clone

SKU MG202414

Cldn9 (tGFP-tagged) - Mouse claudin 9 (Cldn9)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cldn9
• Synonyms: nmf32; nmf329
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202414 representing NM_020293; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTTCCACTGGCCTTGAACTCCTCGGCATGACCCTGGCTGTGCTAGGCTGGCTAGGAACTTTGGTGT; CCTGTGCCCTGCCACTGTGGAAGGTGACCGCCTTCATCGGCAACAGCATCGTTGTGGCCCAAGTGGTATG; GGAGGGGCTGTGGATGTCCTGTGTGGTCCAGAGCACTGGCCAGATGCAGTGCAAAGTATACGACTCACTG; CTGGCGCTGCCCCAGGACCTGCAGGCAGCCAGAGCCCTCTGTGTCGTGGCCCTCCTGCTGGCTTTGCTGG; GCCTGCTGGTGGCTATCACGGGCGCCCAGTGCACCACGTGTGTGGAGGACGAAGGTGCCAAGGCCCGTAT; CGTACTCACCGCAGGGGTCCTCCTCCTCCTCTCGGGCATTTTGGTGCTCATCCCTGTCTGCTGGACAGCC; CATGCCATCATCCAGGATTTTTATAACCCACTGGTTGCGGAAGCCCTCAAGAGAGAACTGGGGGCTTCCC; TCTACCTGGGCTGGGCTGCCGCTGCCCTGCTCATGCTAGGGGGAGGGCTCCTCTGCTGTACGTGTCCCCC; GTCACACTTTGAGCGTCCCCGCGGCCCCAGGCTGGGCTACTCCATCCCTTCCCGTTCAGGTGCTTCGGGA; CTGGATAAGAGGGACTATGTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202414 representing NM_020293; Red=Cloning site Green=Tags(s); ; MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSL; LALPQDLQAARALCVVALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVLLLLSGILVLIPVCWTA; HAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPSHFERPRGPRLGYSIPSRSGASG; LDKRDYV; ; TRTRPLE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020293
• ORF Size: 651 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020293.3, NP_064689.2
• RefSeq Size: 1443 bp
• RefSeq ORF: 654 bp
• Locus ID: 56863
• UniProt ID: Q9Z0S7
• Cytogenetics: 17 A3.3
• Summary: This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is developmentally regulated; it is expressed in neonate kidney, but disappers by adulthood. It is required for the preservation of sensory cells in the hearing organ and the gene deficiency is associated with deafness. [provided by RefSeq, Aug 2010]